J. AOAC Int. 97, 99-104 (2014). HPTLC of pitavastatin calcium (1) and ezetimibe (2) on silica gel with toluene - ethyl acetate - glacial acetic acid 299:200:1. Quantitative determination by absorbance measurement at 235 nm. The hRf values for (1) and (2) were 38 and 58. Linearity was in the range of 50-600 ng/zone for (1) and (2). The intermediate/interday/intra-day precisions were below 2 % (n=3). The LOD and LOQ were 4 and 12 ng/zone for (1) and 4 and 11 ng/zone for (2), respectively. Recoveries were in the range of 98.7-100.1 % for (1) and 99.2-100.0 % for (2).

Chinese J. of Inform. on TCM 20 (1), 54-56 (2013). Zhiqiao Jianwei Keli granule is a herbal TCM for treatment of stagnated heat of liver and stomach, stomach pain and stomach cramps etc. For quality control, TLC on silica gel 1) for Fructus aurantii, with the lower phase of chloroform – methanol – water 13:6:2, detection by spraying with 3 % aluminum trichloride in ethanol and heating at 105 °C for 5 min and evaluation under UV 366 nm; 2) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu, with toluene – acetone 9:2, detection by exposure to iodine vapors for 3 min and evaluation under UV 366 nm; 3) for Forsythia suspensa (Thunb.) Vahl, with chloroform – methanol – glacial acetic acid 17:2:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight. For determination of tetrahydropalmatine, TLC on silica gel with toluene – acetone 9:2 with chamber saturation for 30 min, detection by exposure to iodine vapors for 3 min. Evaluation under UV 366 nm and quantification by densitometric measurement at 343 nm using external standard calibration. Validation by investigating the linearity range of the calibration curve (55-330 ng/zone, r=0.996, n=6), the stability within 6 hours (%RSD 1.9 %, n=7), the precision (%RSD 1.3 %, n=6), and the repeatability (%RSD 0.8 %, n=6). Recovery was 98.0 % (%RSD 1.9 %, n=5).

J. Liq. Chrom. Rel. Tech. 38, 485-491 (2015). HPTLC of (1) salicylic acid and (2) acetylsalicylic acid on RP-18W with mixtures of methanol and water of pH 2.6; the content of methanol in the mobile phase was gradually varied by 10 % from 20–100 % (v/v). The method allowed the estimation of the lipophilicity of (1) and (2).

J. Planar Chromatogr. 27, 444-448 (2014). HPTLC of quercetin in fresh tubers of Satyrium nepalense on silica gel with toluene – ethyl acetate – formic acid 7:5:1. Quantitative determination by absorbance measurement at 366 nm. The hRF value of quercetin was 54. Linearity was in the range of 2-12 μg/zone. The intermediate intra-day and inter-day precisions were below 1 % (n=3) for quercetin. The LOD and LOQ was 40 and 120 ng/zone, respectively. Mean recovery for quercetin was 99.8 %.

Food Res. Int. 65, 13-19 (2014). Review of the challenges and limitations in the analysis of polyphenol-protein interactions. The author described some properties of the HPTLC, such as the level of resolution that can be obtained with 2D-HPTLC and the coupling with mass spectrometry, that make this tool a promising alternative for the analysis of protein-phenol adducts.

Chinese J. Sci. & Technol. Review 17, 220-221 (2013). Zhihe Piba Keli pill is a TCM compound for the treatment of cough. For quality control, microemulsion TLC of Stemona sessilifolia (Miq.) Miq., Morus alba L. and Cynanchum glaucescens (Decne.) Hand.-Mazz on polyamide thin-films with (SDS - n-butanol – n-heptane 2.7:6.3:1.0) – formic acid 9:1, detection at UV 366 nm. Demonstration by applying to real life samples indicated that the procedure was suited for quality control.

J. Chinese Modern Med. & Pharm. 20 (31), 54-56 (2013). Paracetamol and chlorphenamine maleate granule are an anti cold medicine effective for cold induced nasal congestion, headache, sore throat, fever, etc. Artificial bezoar is one of the main ingredients and bilirubin is the key effective component in the medicine. For quality control, TLC of the extracts and bilirubin on silica gel with isooctane – ethyl acetate – glacial acetic acid 11:5:3, detection under white light. Quantitative determination of bilirubin by UV spectrophotometry. Demonstration by applying to real life samples from three pharmaceutical factories indicated that the method was suited for the quality control of the medicine.

Chinese J. Northern Pharm. 10 (12), 10-11 (2013). Yinhuang Qingre Jiaonang capsule is a TCM compound used for the treatment of respiratory tract infection, acute and chronic tonsillitis, sore throat, pneumonia, periodontitis, conjunctivitis, influenza virus infection, etc. For the quality control, HPTLC of the drug and berberine hydrochloride on silica gel (1) for Cortex Phellodendri Chinensis and Coptis chinensis Franch. with benzene – ethyl acetate – isopropanol – methanol – concentrated ammonia 12:6:3:3:1, detection by exposure to ammonia vapor; (2) for Flos Lonicerae Confusae with butyl acetate – formic acid – water 14:5:5; (3) for Fructus Aurantii (A) with ethyl acetate – formic acid – water 100:17:13 up to 3 cm, and then, (B) with toluene - ethyl acetate – formic acid – water 20:10:1:1 up to 8 cm; for all (1-3), detection at UV 366 nm. The method had been applied routinely and proved to be suited for the quality control of the medicine.