J. AOAC Int. 97, 99-104 (2014). HPTLC of pitavastatin calcium (1) and ezetimibe (2) on silica gel with toluene - ethyl acetate - glacial acetic acid 299:200:1. Quantitative determination by absorbance measurement at 235 nm. The hRf values for (1) and (2) were 38 and 58. Linearity was in the range of 50-600 ng/zone for (1) and (2). The intermediate/interday/intra-day precisions were below 2 % (n=3). The LOD and LOQ were 4 and 12 ng/zone for (1) and 4 and 11 ng/zone for (2), respectively. Recoveries were in the range of 98.7-100.1 % for (1) and 99.2-100.0 % for (2).

Chinese J. of Inform. on TCM 20 (1), 54-56 (2013). Zhiqiao Jianwei Keli granule is a herbal TCM for treatment of stagnated heat of liver and stomach, stomach pain and stomach cramps etc. For quality control, TLC on silica gel 1) for Fructus aurantii, with the lower phase of chloroform – methanol – water 13:6:2, detection by spraying with 3 % aluminum trichloride in ethanol and heating at 105 °C for 5 min and evaluation under UV 366 nm; 2) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu, with toluene – acetone 9:2, detection by exposure to iodine vapors for 3 min and evaluation under UV 366 nm; 3) for Forsythia suspensa (Thunb.) Vahl, with chloroform – methanol – glacial acetic acid 17:2:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight. For determination of tetrahydropalmatine, TLC on silica gel with toluene – acetone 9:2 with chamber saturation for 30 min, detection by exposure to iodine vapors for 3 min. Evaluation under UV 366 nm and quantification by densitometric measurement at 343 nm using external standard calibration. Validation by investigating the linearity range of the calibration curve (55-330 ng/zone, r=0.996, n=6), the stability within 6 hours (%RSD 1.9 %, n=7), the precision (%RSD 1.3 %, n=6), and the repeatability (%RSD 0.8 %, n=6). Recovery was 98.0 % (%RSD 1.9 %, n=5).

J. Liq. Chrom. Rel. Tech. 38, 485-491 (2015). HPTLC of (1) salicylic acid and (2) acetylsalicylic acid on RP-18W with mixtures of methanol and water of pH 2.6; the content of methanol in the mobile phase was gradually varied by 10 % from 20–100 % (v/v). The method allowed the estimation of the lipophilicity of (1) and (2).

J. Planar Chromatogr. 27, 444-448 (2014). HPTLC of quercetin in fresh tubers of Satyrium nepalense on silica gel with toluene – ethyl acetate – formic acid 7:5:1. Quantitative determination by absorbance measurement at 366 nm. The hRF value of quercetin was 54. Linearity was in the range of 2-12 μg/zone. The intermediate intra-day and inter-day precisions were below 1 % (n=3) for quercetin. The LOD and LOQ was 40 and 120 ng/zone, respectively. Mean recovery for quercetin was 99.8 %.

Food Res. Int. 65, 13-19 (2014). Review of the challenges and limitations in the analysis of polyphenol-protein interactions. The author described some properties of the HPTLC, such as the level of resolution that can be obtained with 2D-HPTLC and the coupling with mass spectrometry, that make this tool a promising alternative for the analysis of protein-phenol adducts.

J. Planar Chromatogr. 27, 372-376 (2014). For quality control, HPTLC of cefprozil monohydrate on silica gel with ethyl acetate – acetone – methanol – water – glacial acetic acid 15:5:5:3:1. Quantitative determination by absorbance measurement at 280 nm. The hRF value of cefprozil monohydrate was 20. Linearity was between 200 and 5000 ng/zone. The intermediate precision was below 1.5 % (n=3). The LOD and LOQ were 52 and 75 ng/zone, respectively. Recoveries were in the range of 98.7-101.2 %.

J. Chil. Chem. Soc. 59, 2405-2408 (2014). HPTLC of trazodone in human serum on silica gel with toluene - acetone - ethanol - ammonium 18:14:4:1. Quantitative determination by absorbance measurement at 290 nm. The hRF values of trazodone and its metabolite were 82 and 39, respectively. Linearity was between 0.2 and 2 ng/zone. The intermediate intra-day and inter-day precisions were below 3.5 % (n=3). The LOD and LOQ were 16 and 48 pg/zone, respectively. Recoveries for trazodone were in the range of 94.7-99.0 %.

Chinese Trad. Patent Med. 36 (4), 763-766 (2014). Yinchen Siling Keli granule is a TCM preparation for the treatment of chronic active hepatitis B and liver cirrhosis, etc. For quality control, TLC on silica gel (1) for Artemisia capillaris Thunb. with petroleum ether (60-90 ˚C) – ethyl acetate – acetone 5:3:2, detection by spraying with 5 % KOH solution and evaluation under UV 366 nm; (2) for Rheum officinale Baill. and the standards emodin, rhein, and chrysophanol, with the upper phase of petroleum ether (60-90 ˚C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm. Quantitative determination of paeoniflorin, gardenoside, emodin, and chrysophanol by HPLC.