J. Liq. Chromatogr. Relat. Technol. 34, 2664-2673 (2011). HPTLC of curcumin (1) and gallic acid (2) in pharmaceutical formulations containing Curcuma longa Linn and Emblica officinalis extracts, on silica gel with chloroform - ethyl acetate 5:4 + 1 drop formic acid. Quantitative determination by absorbance measurement at 254 nm. The hRf values of (1) and (2) were 55 and 26, respectively. Linearity was between 30-700 ng/zone for (1) and 100-700 ng/zone for (2). LOD and LOQ were 100 and 300 ng/zone for (1), and 33 and 100 ng/zone for (2), respectively. The intermediate/inter-day/intra-day precision (n = 6) was 0.3 % for (1), and 0.1 % for (2). Recoveries (by standard addition) were 98.2-104.0 % for (1) and 97.8-101.5 % for (2).

J. Liq. Chromatogr. Relat. Technol. 35, 1444-1451 (2012). HPTLC of eight new p-toluenesulfonyl-hydrazinothiazole and hydrazine-bis-thiazole derivatives on silica gel RP-18 with mixtures of methanol/water in different methanol proportion between 50 to 70% with 5% increments as mobile phase. Quantitative determination by absorbance measurement at 254 nm. A chromatographic hydrophobicity index was determined and comparable results were obtained using HPLC.

J. Liq. Chromatogr. Relat. Technol. 35, 1325-1335 (2012). HPTLC of tolperisone (1) and its impurities 4-methylpropiophenone (2) and piperidine (3) on silica gel with cyclohexane-1,4 - dioxane - isopropanol - ethanol 32:1:2:8 + 1 drop glacial acetic acid. Detection by dipping in a 0.3 % methanolic ninhydrin solution for 10 min, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 570 nm. The hRf values of compounds (1) to (3) were 10, 76 and 60, respectively. Linearity was in the range of 60-1500 ng/band for (1), 90-400 ng/band for (2) and 40-250 ng/band for (3). Limits of detection and quantification were 20 and 60 ng/band for (1), 30 and 90 ng/band for (2) and 20 and 40 ng/band for (3), respectively.The intermediate precisions (level 2) for (1) to (3) were 1.6 %, 2.6 % and 2.4 % (n=5), respectively. Recovery for compounds (1) to (3) was between 84.6 and 99.7 %.

Hebei J. Trad. Chinese Med. 34 (3), 433-434 (2012). Compound Kuhuang lotion is a TCM preparation used clinically for clearing heat and eliminating dampness, eliminating stagnations of blood, and relieving pains. Description of a method for the quality control of the preparation. TLC of the extracts of the preparations on silica gel 1) for Rheum officinale and the standards emodin, rhein, chrysophanol and aloe emodin, with petroleum ether (60-90 ºC) – ethyl acetate – formic acid 15:5:1, detection in daylight; 2) for Radix Sophorae flavescentis and the standard matrine, with ethyl acetate – propanone – benzene – ammonia 30:10:30:1, detection by spraying with a solution of potassium iodobismuthate – water – hydrochloric acid 10:200:1, detection in daylight; 3) for Golden Cypress and the standard berberine, with benzene – ethyl acetate – methanol – isoamyl alcohol – water 20:10:5:5:1, detection at UV 366 nm.

J. Liq. Chromatogr. Relat. Technol. 35, 2753-2764 (2012). HPTLC of melitracen hydrochloride (1) and flupentixol hydrochloride (2) on silica gel with methanol - chloroform - toluene 2:9:9 + 1 drop ammonia. Quantitative determination by absorbance measurement at 291 nm. The hRf value of compounds (1) and (2) were 53 and 34 and selectivity regarding matrix was given. Linearity was in the range of 1600-6400 ng/band and 80-320 ng/band for (1) and (2), respectively. Limit of detection was found to be 27 ng/band and 5 ng/band for (1) and (2), respectively. Limit of quantification was found to be 82 ng/band and 14 ng/band for (1) and (2), respectively. The intermediate/inter-day/intra-day precision was below 0.2 % for (1) and 1.1 % for (2) (n=3). Recovery (by standard addition) was between 99.1 and 101.8 % for both (1) and (2). The method showed comparable results with HPLC.

J. Planar Chromatogr. 25, 559-565 (2012). HPTLC of zafirlukast on silica gel with toluene - methanol - acetone 7:2:1. Quantitative determination by absorbance measurement at 240 nm. Linearity was in the range of 50-400 ng/zone. Limits of detection and quantification were 16 and 50 ng/zone, respectively.

J. Planar Chromatogr. 25, 523-527 (2012). TLC of a mixture of 12 non-steroidal anti-inflammatory drugs on silica gel with ethyl acetate - toluene - methanol - acetic acid 40:40:1:1. Quantitative determination by absorbance measurement at 254 nm. Limits of detection were in the range of 5-500 ng/zone. The method showed comparable results to a validated HPLC method.

J. of Qilu Med. & Pharm. 31 (6), 327-330 (2012) Zhachong Shisanwei Pill is a herbal TCM preparation for invigorating the circulation of blood. TLC on silica gel 1) for Fructus Chebulae and the standard gallic acid, with chloroform – ethyl acetate – formic acid 3:2:1, detection by spraying with 2 % ferric chloride in ethanol and heating at 105 ºC until the zones are visible in daylight; 2) for Radix Aucklandiae and the standards costunolide and dehydrocostus lactone, with chloroform – cyclohexane 5:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 ºC until the zones are visible in daylight; 3) for Aquilaria agallocha (Lour.) Roxb, with toluene – ethyl acetate 4:1, detection under UV 366 nm; 4) for Rhizoma Acori Tatarinowii, with petroleum ether (60-90 ºC) – ethyl acetate 8:3, detection under UV 366 nm.