J. of China Pharm. 21 (14), 41-43 (2013). Jinmaxing Zhikepian tablets are a herbal TCM preparation for the treatment of asthma, and acute and chronic bronchitis. For quality control, TLC on silica gel 1) for Peucedanum praeruptorum Dunn, Scutellaria baicalensis and Herbal Ephedrae, with chloroform – methanol – ammonia 40:8:1, detection a) under UV 366 nm and 254 nm, b) by spraying with 2 % ninhydrin in ethanol and heating at 100 °C until the zones are visible in daylight; 2) for Lonicera Japonica, with the upper phase of ethyl acetate – formic acid – water 3:1:1, detection by heating at 100 °C for 5 min and viewing under UV 254 nm; 3) Eriobotrya japonica Thunb., with cyclohexane – ethyl acetate – formic acid 150:40:3, detection by spraying with 10 % sulfuric acid in ethanol and heating at 100 °C until the zones are visible in daylight; 4) for Semen Armeniacae Amarum, with cyclohexane – ethyl acetate – formic acid 150:20:1, detection by spraying with 2 % phosphomolybdic acid in ethanol and heating at 100 °C until the zones are visible in daylight; 5) for Platycodon grandiflorus, with chloroform – ethyl acetate – methanol – ammonia 4:1:4:1, detection by spraying with 5 % ninhydrin in sulfuric acid – ethanol 1:6 and heating until the zones are visible in daylight. Quantification of pseudoephedrine hydrochloride, ephedrine hydrochloride and vitamine B17 by HPLC.

in Shanhaidanpian tablets) (Chinese). J. of China Pharm. 26 (9), 997-999 (2012). Shanhaidanpian tablets are a herbal TCM preparation for the treatment of poor blood flow, blood stasis and chest pain syndrome. For quality control, TLC on silica gel with toluene – ethyl acetate – formic acid 20:2:1, detection by exposure to vapors until the zones are visible in daylight. Quantitative determination of 2,3,5,4’-tetrahydroxystilbene-2-O-beta-D-glucoside by HPLC.

J. Planar Chromatogr. 26, 427-434 (2013). HPTLC of oseltamivir (1) in pharmaceuticals counterfeited with ascorbic acid (2) on silica gel with methanol - water 3:2 +1 drop ammonia. Quantitative determination by absorbance measurement at 254 nm. The hRf values for (1) and (2) were 70 and 83, respectively. Linearity was between 5 and 14 µg/zone. LOD and LOQ were 2 and 5 µg/zone. Average recovery (by standard addition) was found to be 100.6 %. Intermediate intra- and inter-day precision was below 1.6 %. Comparing with colorimetric method, HPTLC method provided advantages in terms of speed, lower cost, and environmental protection without sacrificing accuracy.

J. Liq. Chromatogr. Relat. Technol. 37, 2814-2828 (2014). HPTLC of arylureas and arylacetamides derivatives on RP-18 with 1-13 % water in dimethylsulfoxide (increment 3 %). Detection under UV 254 nm. A quantitative structure-retention relationship study allowed to investigate the retention behavior of these substances as well as determination of lipophilicity related to the biological activity towards dopamine D2 and 5-hydroxy-tryptamine receptors.

J. Liq. Chromatogr. Relat. Technol. 37, 1524-1534 (2014). HPTLC of haloperidol on RP-18 with acetonitrile - water - 1-alkyl-3-methylimidazolium tetrafluoroborate 120:80:3. Quantitative determination by absorbance measurement at 245 nm. The hRF value for haloperidol was 57. Recoveries were in the range of 99.4-106.5 %.

Chinese J. of Prac. Med. 8 (25), 1-3 (2013). Gangmei Ganmaoling keli granule is herbal TCM for the treatment of colds, upper respiratory tract infections etc. For quality control TLC on silica gel 1) for Holly roots, with toluene – ethyl acetate – glacial acetic acid 10:3:1, detection under UV 366 nm and under daylight after exposing to ammonia vapors; 2) for Dendranthema indicum and the standard buddleoside, with ethyl acetate – butanone – chloroform – formic acid – water 15:15:6:4:1, detection by spraying with 2 % aluminum trichloride in ethanol and heating until the zones are visible under UV 365 nm; 3) for Isatis tinctoria and the standard arginine, with n-butanol – glacial acetic acid – water 19:5:5, detection by spraying with 2 % ninhydrin in acetone and heating at 105 ˚C until the zones are visible in daylight.

Braz. J. Pharm. Sci. 49, 845-851 (2013). HPTLC of metoprolol (1) and hydrochlorothiazide (2) in human plasma on silica gel with chloroform - methanol - ammonia 18:2:1. Quantitative determination by absorbance measurement at 239 nm. The hRF values for (1) and (2) were 48 and 13. Linearity was in the range of 20-120 μg/zone for (1) and 2-12 μg/zone for (2). The intermediate/interday/intra-day precisions were below 5 % (n=3). The LOQs were 20 and 2 μg/zone for (1) and (2), respectively. Recoveries were between 70.6 and 84.9 %.

J. Planar Chromatogr. 27, 58-65 (2014). HPTLC of ternary mixtures of cetirizine dihydrochloride (1), pseudoephedrine (2), and/or phenylpropanolamine (3) and paracetamol (4) on silica gel with methanol - water 199:1. Quantitative determination by absorbance measurement at 257 nm. The hRF values for compounds (1) to (4) were 66, 16, 23 and 83, respectively. Linearity was in the range of 1-4 µg/zone for (1), 3-10 µg/zone for (2), 4-8 µg/zone for (3) and 5-100 µg/zone for (4), respectively. The intermediate/interday/intra-day precisions were below 2 % (n=5). The LOD and LOQ were 0.3 and 1 µg/zone for (1), 0.9 and 3 µg/zone for (2), 1.2 and 4 µg/zone for (3) and 1.5 and 5 µg/zone for (4), respectively. Mean recoveries for (1) to (4) were close to 100 %.