J. Planar Chromatogr. 30, 460-466 (2017). HPTLC of cholesterol oleate, glycerol trioleate, squalene, β-sitosterol, linoleic acid, glycosylsterol (β-sitosterol glucoside), glycosylceramide, protocatechuic acid and quercetin 4ʹ-O-β-d-glucopyranoside (2) on silica gel with chloroform ‒ methanol 17:3. Detection by dipping into a solution of copper(II) sulfate (10 %), phosphoric acid (8 %), and methanol (5 %) in water, followed by heating at 150 °C for 10 min. Quantitative determination by absorbance measurement at 546 nm. Linearity was between 25 and 1000 ng/zone. LOD and LOQ were between 50 and 125 ng/band and 125 and 250 ng/band, respectively. The intermediate precision was <2 % (n=6). Recovery ranged from 106.5 % to 112.1 %.

J. Chromatogr. A 1524, 266-272 (2017). Demonstration of the antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds. Investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria by TLC and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography. Transfer of antibacterial fractions obtaining infusion-transfusion OPLC to HPLC-MS/MS analysis resulted in the characterization of three active compounds, two of them were identified as linoleic and linolenic acid. Adoption of OPLC method to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide, sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.

J. Planar Chromatogr. 31, 155-162 (2018). HPTLC of lawsone in Lawsonia inermis on silica gel with benzene ‒ ethyl acetate ‒ acetic acid 75:25:1. Quantitative determination by absorbance measurement at 275 nm. The hRf value for lawsone was 33. Linearity was in the range of 50-350 ng/zone. The intermediate precision was below 2 % (n=6). The LOD and LOQ were 16 and 50 ng/zone for lawsone, respectively. Average recovery was 96 %.

J. Planar Chromatogr. 31, 197-201 (2018). HPTLC of shatavarin IV in the roots of Asparagus racemosus on silica gel with ethyl acetate – methanol – water 15:3:2. Detection by spraying with anisaldehyde sulfuric acid reagent, followed by heating at 105 ºC for 5 min. Quantitative determination by absorbance measurement at 425 nm. The hRf value for shatavarin IV was 43. Linearity was in the range of 600-1800 ng/zone. The intermediate precision was below 2 % (n=3). The LOD and LOQ were 14 and 44 ng, respectively. Average recovery was 96.2 %.

J. AOAC Int. 101, 1437-1447 (2018). Micro-TLC of 18 standard dyes (Amaranth, Bromophenol blue, Bromothymol blue, Patent blue V, p-Xylenol blue, Brilliant Black BN, Erythrosine, Fluorescein, Carmine, Naphthalene black 10B, Phenol red, Bromocresol purple, Sudan II, Sudan III, Sudan IV, Bromocresol green, Dimethyl yellow, and Methyl red) on silica and cellulose with different solvent mixtures (methanol – water and dichloromethane – methanol) in proportions varying from 0 to 100 %. Chromatographic parameters and quantum mechanics properties of each solute were used along with data mining to model the chromatographic behavior.

ex Schult. Pharmacogn. Mag. 14, 630-637 (2018). HPTLC of quercetin (1), kaempferol (2) and myricetin (3) in the aerial parts and roots of Aerva lanata on silica gel with toluene – ethyl acetate – formic acid 12:8:1. Quantitative determination by absorbance measurement at 254 nm. Linearity ranged between 100-1000 ng/zone for (1) and 25-1250 ng/zone for (2) and (3).

J. Planar Chromatogr. 31, 383-388 (2018). HPTLC of cocaine hydrochloride (1) and levamisole hydrochloride (2) on silica gel with cyclohexane – toluene – diphenylamine 75:15:10. Quantitative determination by absorbance measurement at 230 nm. The hRF values for (1) and (2) were 24 and 48, respectively. Linearity was between 200 and 2400 ng/zone for (1) and 100 and 1200 ng/zone for (2). LOD and LOQ were 14 and 42 ng/zone for (1) and 6 and 19 ng/zone for (2), respectively. The intermediate precision was <2 % (n=6). Average recovery was 99.8 % for (1) and 99.9 % for (2).

Braz. J. Pharm. Sci. 54, 1-10 (2018). HPTLC of gliquidone on silica gel with chloroform – cyclohexane – glacial acetic acid 6:3:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for gliquidone and degradation products I, II and III were 60, 1, 24 and 52, respectively. Linearity was between 2 and 20 μg/zone. LOD and LOQ were 261 and 791 ng/zone. The intermediate precision was <2 % (n=3). Average recovery was 100.0 %.