Beijing J. Trad. Chinese Med. 32 (6), 457-459 (2013). Qingfei Wan pill is a traditional Chinese compound herbal medicine prescribed for the treatment of fever, cough, runny nose, sore throat, etc. For quality control, TLC on silica gel (1) for Gardenia jasminoides Ellis and gardenoside with chloroform – methanol – ethyl acetate – ammonia 20:8:4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating until the zones are visible; (2) for Belamcanda chinensis L. Redouté with chloroform – methanol – ammonia 40:10:1, detection at UV 254 nm; (3) for Terminalia chebula Retz. with chloroform – ethyl acetate – formic acid 6:4:1, detection by spraying with 2 % ferric chloride in ethanol, evaluation under white light.

Chinese J. Med. Pharm. 4 (1), 120-122 (2014). Caihuang Shuangjia Keli granules are a TCM preparation with antibacterial and antiviral effects especially on influenza virus and adenovirus. For quality control, TLC on silica gel (1) for Rheum palmatum L. with petroleum ether (30-60 °C) – methyl acetate – formic acid 15:5:1, detection at UV 366 nm; (2) for Artemisia carvifolia with petroleum ether (60-90 °C) – ethyl acetate 3:2, detection at UV 366 nm; (3) for Scutellaria baicalensis Georgi and baicalin with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 10 % ferric chloride in ethanol and viewing in daylight; (4) for Isatis indigotica Fortune and indigo blue with toluene – benzene - ethyl acetate – chloroform 2:2:1:4, detection in daylight; (5) for Amomum tsao-ko with toluene - ethyl acetate 9:4, detection by spraying with 10 % aluminum phosphate in ethanol and heating at 105 °C for 5 min and viewing in daylight.

J. Liq. Chromatogr. Relat. Technol. 38, 1392-1406 (2015). HPTLC of Cordyceps sinensis and Ganoderma lucidum on silica gel with ethyl acetate - dichloromethane - formic acid - glacial acetic acid - methanol 10:10:1:1:2. Qualitative identification at UV 254 nm and UV 366 nm. Hyphenation of electronspray ionization/mass spectrometry with HPTLC facilitated fast and convenient profiling of the metabolites in the extracts. For the chemometric analysis, raw and column data matrices were constructed using hRF datasets.

and species comparison study using high-performance thin-layer chromatography - mass spectrometry. J. Planar Chromatogr. 28, 280-286 (2015). HPTLC of trichostachine (1), piperine (2), 4,5-DHPL (3), guineensine (4), pellitorine (5) and sesamin (6) in Piper longum, Piper chaba and Piper nigrum on silica gel with ethyl acetate - n-hexane - dimethyl amine 40:60:1. Quantitative determination by absorbance measurement at 273 nm for (4) and (5), 294 nm for (3) and (6) and 340 nm for (1) and (2). The method was coupled with MS to identify the 6 markers. The hRF values for (1) to (6) were 12, 36, 46, 71, 78 and 82, respectively. Linearity was in the range of 25-1000 ng/zone for (1) to (6). LOD was between 4 and 7 ng/zone whereas LOQ was between 14 and 20 ng/zone for (1) to (6). The intermediate precision was below 1 % (n=4). Recoveries were in the range of 99-103 %.

J. Chromatogr. Sci. 53 (2), 312-319 (2015). Investigation of lipophilicity for newly synthesized N-substituted-2-phenylacetamide derivatives by TLC on RP-18 in the presence of one protic (methanol) and one aprotic solvent (acetonitrile) followed by mathematical evaluation using different software packages. Establishment of the dependences between the partition coefficient, log P as the standard measure of lipophilicity and the alternative lipophilic parameters using the retention chromatographic constant RM0 and m values (the intercept and the slope of the linear dependence between volume fractions of the organic solvent and the retention parameters obtained experimentally by TLC), employing linear regression analysis and multivariate methods, cluster analysis (CA) and principal component analysis (PCA). All applied methods gave approximately similar results. Although there is a linear dependence between the two chromatographic parameters RM0 and m, only RM0 shows suitable similarity with the standard measure of lipophilicity of the investigated N-substituted-2-phenylacetamide derivatives at the given conditions. This proves that the chromatographic retention constant, RM0, obtained by reversed phase TLC could be successfully used for the description of lipophilicity of the compounds. The results confirmed that the applied linear regression analysis and the multivariate analysis (CA and PCA) have the ability to compare lipophilic parameters of the investigated phenylacetamide derivatives obtained in different ways.

J. Sep. Sci. 38, 4180-4186 (2015). HPTLC of glycyrrhizic acid in the dried root and rhizoma of the plants Glycyrrhiza uralensis Fisch, Glycyrrhiza inflata Bat, and Glycyrrhiza glabra L. on silica gel with ethyl acetate - water - acetic acid - formic acid 14:2:2:1. Detection by spraying with 10 % solution of sulfuric acid in ethanol, followed by heating at 105 °C for 10 min. Quantitative determination by absorbance measurement at 365 nm.

J. Planar Chromatogr. 28, 395-397 (2015). HPTLC of 25-B-NBOMe (a N-(2-methoxy)benzyl-substituted phenylethylamine hallucinogen) in seized blotters (small, square pieces of absorbant paper impregnated with LSD or other hallucinogens) on silica gel with cyclohexane – toluene – diethylamine 15:3:2. Quantitative determination by absorbance measurement at 298 nm. The hRF value for 25-B-NBOMe was 34. Linearity was in the range of 19-115 μg/zone. LOD and LOQ were 7 and 22 μg/zone. The intermediate precision was below 6.3 % (n=6). Average recovery was 98 %.

J. Ethnopharmacol. 190, 116-141 (2016). This review discusses the progress in chemical analysis of Angelica sinensis and its preparations. The study describes TLC as a rapid separation method and qualitative analysis technology that is commonly used for routine chemical analysis of A. sinensis. The advantages of the technique as well as TLC systems using ferulic acid and ligustilide as markers were described.