Chinese J. of Med. Guide 10 (20), 99-102 (2013). Fufang Xiaoji Zhike Milian Gao ointment is herbal TCM preparation for clearing the body heat caused by inflammation, resolving phlegm and relieving asthma. For quality control, TLC on silica gel 1) for Pericarpium Citri Reticulatae and the standard hesperidin, first over 3 cm with ethyl acetate – methanol – water 99:17:13 and thenover 8 cm with toluene – ethyl acetate – formic acid – water 95:45:5:3, detection by spraying with 2 % aluminium chloride in ethanol and viewing under UV 366 nm; 2) for Schisandra Chinensis and the standard schizandrin A, with the upper phase of petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 254 nm; 3) for Crataegus pinnatifida and the standard ursolic acid, with toluene – ethyl acetate – formic acid – water 39:8:2:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 80 °C, detection under UV 366 nm. Quantification of tenuifolin by HPLC.

Food Chem. 156, 94-99 (2014). TLC of epigallocatechin gallate (1), gallic acid (2) and caffeine (3) on silica gel with dichloromethane - acetone - formic acid 8:7:1. Detection by dipping into 5 % methanol - sulfuric acid reagent, followed by heating at 105 °C for 3 min. Quantitative determination of (1) as gallic acid equivalents by absorbance measurement at 270 nm (epigallocatechin gallate (EPCG) was calculated and expressed as gallic acid equivalents because the EPCG standard is very expensive). The hRF values for (1) to (3) were 54, 69 and 80. Linearity was in the range of 500-5000 ng/zone for (2) and 200-2400 ng/zone for (3). The intermediate/inter-day/intra-day precisions were below 2 % (n=3). The LODs and LOQs were 400 and 500 ng/zone for (2) and 180 and 200 ng/zone for (3), respectively. Recoveries were between 94.9 and 100.0 % for (2).

Phytochemistry 105, 68-78 (2014). HPTLC of antifungal compounds in Morinda tormentosa on silica gel with dichloromethane - methanol - ethyl acetate 43:6:1. Bioautography with Candida albicans DSY2621 or parent wild type CAF2-1 at 37 ºC overnight, followed by spraying with an aqueous solution (2.5 mg/ml) of thiazolyl blue (methyl thiazolyl tetrazolium chloride) and incubation for 6 h at 37 ºC. Clear inhibition zones were observed against a purple background.

Chinese J. of Inform. on TCM 20 (3), 67-69 (2013). Zishen Jiaonang capsule is a herbal TCM effective in tonifying the liver and kidney, and is prescribed for premature ovarian failure, acute and chronic hepatitis, mastitis, sterility, etc. For quality control, HPTLC of the extracts of the preparation on silica gel 1) for Paeonia lactiflora Pall. and the standard paeoniflorin, with chloroform – methanol – water 14:6:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight; 2) for Ziziphus jujuba Mill. Var. spinosa, with water-saturated n-butanol, detection under UV 366 nm; 3) for Lilium brownii var. viridulum with petroleum ether (60-90 °C) – ethyl acetate – formic acid 15:5:1, detection by spraying with 10 % phosphomolybdic acid hydrate in ethanol and heating mildly until the zones are visible in daylight; 4) for Caulis polygoni multiflori and the standard emodin, with n-hexane – ethyl acetate – formic acid 60:20:1, detection under UV 366 nm. Quantification of paeoniflorin by HPLC.

J. Planar Chromatogr. 27, 52-57 (2014). HPTLC of paracetamol (1), dicyclomine hydrochloride (2) and mefenamic acid (3) on silica gel with toluene - ethyl acetate - methanol 6:3:1. Quantitative determination by absorbance measurement at 290 nm. The hRF values for (1) to (3) were 27, 41 and 61, respectively. Linearity was in the range of 600-1800 ng/zone for (1), 400-2400 ng/zone for (2) and 300-1500 ng/zone for (3). The intermediate/interday/intra-day precisions were below 2 % (n=3). The LOD and LOQ were 35 and 116 ng/zone for (1), 109 and 360 ng/zone for (2) and 42 and 140 ng/zone for (3), respectively. Recovery was in the range of 97.7-99.9 % for (1) to (3).

Chinese J. of Henan Univ. (Med Sci.) 32 (3), 184-186 (2013). Mingmu Keli granule is a traditional Chinese compound herbal medicine prescribed clinically to treat impaired vision, red eye and eye dryness, macular degeneration, etc. For quality control, TLC 1) for Rhizoma Atractylodis, on silica gel with petroleum ether (60-90 °C) – ethyl acetate 20:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in sulfuric acid – water 1:10 and heating mildly until the zones are visible in daylight; 2) for Citrus maxima (Burm.) Merr. cv. Tomentosa, on silica gel first over 3 cm with ethyl acetate – methanol – water 100:17:13, then over 8 cm with toluene – ethyl acetate – glacial acetic acid – water 40:20:3:2, detection under UV 366 nm after spraying with 3 % aluminum trichloride in ethanol; 3) for Saposhnikovia divaricata (Trucz.) Schischk., on silica gel (with fluorescence indicator F254 nm) with chloroform – methanol 8:3, detection under UV 254 nm. Quantification of paeoniflorin by HPLC.

J. Planar Chromatogr. 27, 420-427 (2014). HPTLC and TLC of fatty acids and polyphenols in plant extracts of raspberry (Rubus idaeus), strawberry (Fragaria ananassa), blackcurrant (Ribes nigrum), aronia (Aronia Medik.), Japanese rose (Rosa rugosa Thunb.) seeds, and palmetto palm (Sabal minor) fruit on silica gel or RP-18 with different micellar mobile phases containing cationic cetyltrimethylammonium bromide (CTBA), or anionic sodium dodecyl sulfate (SDS), or nonionic polioxyethylene (23) lauryl ether (Brij35).

J. Planar Chromatogr. 27, 449-453 (2014). HPTLC of the glycome of different species of Panax (P. ginseng, P. notoginseng, and P. quinquefolium). Monosaccharides were analyzed on cellulose phase with ethyl acetate - pyridine - acetic acid - water 35:15:1:7 for the first development and 35:15:1:9 for the second development. Detection by spraying with aniline-O-phthalic acid reagent (containing 1.6 g o-phthalic acid dissolved in 100 mL water-saturated n-butanol, containing 0.9 mL of aniline), followed by heating at 105 °C for 5 min. Oligosaccharides were analyzed on silica gel with n-butanol - isopropanol - acetic acid - water 7:5:1:2. Detection by spraying with diphenylamine–aniline–phosphoric acid solution (4 g diphenylamine mixed with 4 mL aniline and 20 mL 85% (v/v) phosphoric acid in 200 mL acetone), followed by heating at 105 °C for 5 min._x000D_