Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
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Journal of Chromatography B, 1184, 122956 (2021). Test for acetyl- and butyrylcholinesterase (AChE and BChE) inhibition without development of piperin (standard inhibitor of AChE and BChE) and ethanol – water (3:2) extracts of Iranian plants, on HPTLC silica gel prewashed twice with methanol – water 3:2 and dried 60 min at 120°C. After sample application the plate was immersed (speed 3.5 cm/s, time 2 s) into enzyme solution (6.6 units/mL AChE or 3.3 units/mL BChE in TRIS buffer 0.05 M, with bovine serum albumin 0.1 %, pH 7.8), incubation 25 min at 37°C and immersion (speed 3.5 cm/s, time 1 s) into chromogenic substrate solution (α-naphthyl acetate 0.1 % and Fast Blue salt B 0.2 % in ethanol – water, 1:2). Seven mobile phases were tested for the active samples. Best separation was obtained with toluene – ethyl acetate – formic acid – water 4:16:3:2 and with toluene – ethyl acetate – methanol 6:3:1. Before enzymatic assay, plates developed with acidic mobile phases were neutralized by spraying 3 mL citrate phosphate buffer (Na2HPO4 8 %, citric acid q.s. ad pH 7.5) followed by 10 min of automatic drying. Enzymatic assay was performed using a piezoelectric spraying device: a) pre-wetting by spraying 1 mL TRIS buffer (0.05 M, pH 7.8); b) spraying 3 mL of the enzyme solution; c) incubation 25 min in a humid box at 37°C; d) spraying 0.5 mL substrate solution; e) 5 min drying at room temperature, and then 10 min of automatic drying. By spraying, zone shift and zone diffusion, which occurred with plate immersion, were avoided. For development control, derivatization was done by piezoelectrically spraying 4 mL of sulfuric anisaldehyde reagent (anisaldehyde – sulfuric acid – acetic acid – methanol, 1:10:20:170), followed by heating 3 min at 110°C. For identification of zones of interest, direct elution with methanol from underivatized HPTLC plates through a TLC-MS interface directly to a MS. Identified zones were 3-O-acetyl-β-boswellic acid (triterpenoid) from Boswellia carteri gum-resin (Burseraceae), pimpinellin and psoralen (furocoumarins) from Heracleum persicum flowers (Apiaceae), oleuropein (seco-iridoid) from Olea europaea leaves (Oleaceae), harmine, harmaline, vasicine, deoxyvasine (alkaloids) from Peganum harmala seeds (Zygophyllaceae), costic acid (sesquiterpene) from Nardostachys jatamansi hypocotyl (Valerianaceae), elaidic, linoleic, palmitic, palmitoleic acids (fatty acids) from Pistacia atlantica fruits (Anacardiaceae).
J. Planar Chromatogr. 34, 447-453 (2021). HPTLC of gallic acid (1) and ellagic acid (2) in Triphala on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:5:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 35 and 28, respectively. Linearity was between 200 and 2000 ng/zone for (1) and 50 and 400 ng/zone for (2). LOD and LOQ were 200 and 606 ng/zone for (1) and 50 and 151 ng/zone for (2), respectively. Intermediate precisions were below 2 % (n=3). Average recovery was 98.6 % for (1) and 99.1 % for (2).
J. Sep. Sci. 44, 3146-3157 (2021). HPTLC of gallic acid (1), cinnamic acid (2), piperine (3), eugenol (4), and glycyrrhizin (5) in Divya-Swasari-Vati on silica gel with ethyl acetate - toluene - formic acid 10:9:1 for (1) to (4) and ethyl acetate - formic acid - acetic acid - water 100:10:10:23 for (5). Quantitative determination by absorbance measurement at 280 nm for (1), (2) and (4), 343 nm for (3) and 254 nm for (5). The hRF values for (1) to (5) were 31, 64, 53, 70 and 29, respectively. Linearity was between 400 and 800 µg/mL for (1), 5 and 25 µg/mL for (2), 600 and 1400 µg/mL for (3), 400 and 1200 µg/mL for (4) and 100 and 800 µg/mL for (5). Intermediate precision was below 2 % (n=18). The LOD and LOQ were 180 and 560 ng/g for (1), 3 and 10 ng/g for (2), 8 and 26 µg/g for (3), 3 and 11 µg/g for (4) and 300 and 920 ng/g for (5), respectively. Mean recovery was 96.0 % for (1), 92.3 % for (2), 94.2 % for (3), 96.2 % for (4) and 94.6 % for (5).
J. Ethnopharmacol. 275, 114054 (2021). HPTLC of ellagic acid in Anogeissus latifolia on silica gel with toluene - ethyl acetate - formic acid 20:5:1. Detection by spraying with anisaldehyde - sulphuric acid reagent, followed by heating at 105 °C. The hRF value for ellagic acid was 38.
Chinese J. Food & Drug 23 (5), 411-415 (2021). Dashanzha tablet is a Chinese patent medicine with the function of appetizing and digestion, used for food accumulation caused by loss of appetite, indigestion, abdominal distension. For quality control, TLC of the petroleum ether extracts on silica gel with cyclohexane - methylene dichloride - ethyl acetate - glacial acetic acid 200:50:80:1. Detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ℃ until the zones are visible in daylight. Identification by fingerprint comparison with the standard ursolic acid and the standard ingredient drug undergone the same procedure in parallel. Satisfactory results were achieved by using plates from different manufactures and under varying temperature and humidity conditions.
J. Spectroscopy & Spectral Anal. 41 (2), 388-394 (2021). SERS, as a fast and sensitive analytical technology, is widely employed in the fields of analytical chemistry, environmental detection and food safety. However, the real-life samples are mostly mixtures, and an accurate determination of the analytes in complex samples cannot be performed directly by using SERS. TLC as a separation technique is easy to operate, low cost, fast and high-throughput, and has been widely used in the fields of synthetic chemistry, analytical chemistry, medicinal chemistry, and food science. Further, the zones isolated by TLC are first visualized using iodine vapor coloring or fluorescence, and then combined with SERS for efficient qualification and quantitation of the zones of interest. Therefore, the technology of TLC combined with SERS (TLC/SERS) suits rightly for determination of various kinds of complex samples. Moreover, due to the small sample size and the relatively simplicity of the experimental equipment used, it is also suitable for the rapid field screening and detection of relatively complex samples. Introduction of the enhancement mechanism of SERS and the preparation of the active substrate, and demonstration of the broad prospects of TLC/SERS application in the fields of environmental pollutant analysis, food safety monitoring, traditional Chinese medicine and biomedicine identification etc by providing a set of successful application examples.
Chinese J. Ethnomed. & Ethnopharm. (10), 36-39 (2021). Huangjia Ruangan granules is a TCM preparation, which relaxes the liver and promotes blood circulation, and is used for treating liver fibrosis and early cirrhosis, spleen deficiency, liver depression and blood stasis. (A) Identification of Salvia miltiorrhiza Bge, Pueraria lobata (Willd.) Ohwi, and Paeoniae rubra root, by TLC of the methanol extracts, the control solutions lacking the three drugs, and the standards tanshinone IIA, puerarin, paeoniflorin, on silica gel first with chloroform – methanol – water 70:25:2.5 to 5 cm and then with toluene – ethyl acetate 24:1 to 8 cm; detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:200 and heating at 105 ˚C until the zones are visible in white light and UV 365 nm. (B) Identification of Astragali Radix, Panax notoginseng (Burkill) F. H. Chen ex C. H.) and Radix Bupleuri, by TLC of the methanol extracts, the control solutions lacking the three drugs and the standards astragaloside lV, notoginsenoside R1 and saikosaponin A, on silica gel with butanol – ethyl acetate – water 4:3:5 to 17 cm, detection by spraying with 2 % ethanol p-dimethylaminobenzaldehyde solution - sulfuric acid 2:3 and heating at 80 ˚C until the zones are visible, evaluation in white light and at UV 365 nm. The method was successfully applyed to identify six major ingredients in Huangjia Ruangan granules on the same plate through multi-information obtained, and proved to be simple, fast, specific, reproducible, robust and well suitable for the purpose.
J. Trad. Chinese Veterinary Med. 55 (1), 15-19 (2021). Qixingcha Keli is a TCM preparation converted from human to animal use, for the treatment of gastric stagnation in piglets and other animals. Quality control of its component (A) Crataegus L. by TLC of the ethanol extracts, the control solutions with and without Crataegus L. on silica gel with ethyl ether - chloroform - formic acid 5:5:1 to 9 cm. Detection by heating the plates at 105 ˚C for 15 min, then spraying with 0.05 % bromophenol solution (pH = 7.5) and viewing in white light. For component (B) Glycyrrhiza uralensis Fisch., TLC of the ethanol extracts, the control solutions with or without Glycyrrhiza uralensis Fisch. on silica gel containing 1 % NaOH with ethyl acetate - formic acid - glacial acetic acid - water 15:1:1:1 to 18 cm, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ˚C, evaluation in white light and at UV 365 nm. For (C) Uncaria rhynchophylla (Miq.) Miq. ex Havil., TLC of the ethanol extracts and the control solutions on silica gel with petroleum ether (60-90 ˚C) - propanone 3:2 to 9 cm, detection at UV 254 nm. For (D) Semen Coicis, by TLC of the ethyl acetate extracts and control solutions on silica gel with petroleum ether (60-90 ˚C) - ethyl ether - glacial acetic acid 166:34:1 to 9 cm, detection by spraying with 5 % vanillin in sulfuric acid - ethanol 1:200 and heating at 105 ˚C, detection in white light and at UV 365 nm.