Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      131 086
      (Development of a method for identification and source tracking of Ganoderma lucidum growing up in Fujian province by HPTLC) (Chinese)
      Y. ZHANG (Zhang Ying), K. JIANG (Jiang Kunxia), M. GUAN (Guan Mengyao), CH. WU (Wu Changhui), W. XU (Xu Wen)*, W. XU (Xu Wei), Y. LIN (Lin Yu), Q. CAI (Cai Qinqin) (*Cent. of Biomed. R&D, Fujian Univ. of Trad. Chinese Med., Fuzhou 350122, China,

      Chinese J. Pharm. Research 42 (2), 98-104 (2023). Ganoderma lucidum is the dry fruiting body of fungus, a medicinal plant, having the effect of relieving asthma and relieving nerves. Ganoderma lucidum is widely distributed in China and mainly concentrated in Zhejiang, Anhui, Shandong, Jilin, and Fujian. In this study, the HPTLC method for quality detection and origin traceability was established towards the medicinal material produced in Fujian and other areas. HPTLC of the ethanol extracts of the sample materials on silica gel with chloroform – acetonitrile – methanol – formic acid 130:15:2:2, three times to distances of 3 cm, 6 cm, 8 cm. Detection by spraying with 3 % sulfuric acid in ethyl acetate and heating at 105°C until the bands are clearly visualized, evaluation at UV 366 nm. The bands on the thin layer chromatogram were identified by fingerprint comparison using standard samples, and cluster analysis for the data of samples from different producing areas was performed using digital characterization. As results, the HPTLC analysis detected 17 distinct bands and among them, 11 components were identified, e.g. ganoderenic acid C (hRf 0.31), ganoderic acid C2 (hRf 33), ganoderic acid l (hRf 35), ganoderic acid G (hRf 41), ganoderic acid A (hRf 44), ganoderic acid B (hRf 46), ganolactone B (hRf 53), 3β,7β,15β-trihydroxy-11,15-dihydroxy-lanostane-8-alkene-24→20 lactone (hRf 56), ganoderenic acid D (hRf 61), ganoderic acid D (hRf 63) and 20 (21)-dehydrolucidenic acid A (hRf 65). A total of 10 HPTLC bands were identified in Fujian Ganoderma lucidum, among which ganoderic acid C2, ganoderic acid D and two unknown component bands (hRf 14 and 17) were unique and could be used as markers of Fujian Ganoderma lucidum. Thus samples of Fujian Ganoderma lucidum can be differentiated from those of other areas, when the Euclidean distance is 25 in Euclidean cluster analysis.

      Classification: 32e
      131 088
      (Study of the method for the quality control of Ganqingqinglan (Dracocephalum tanguticum Maxim.), a traditional Tibetan medicinal plant, by thin-layer chromatography) (Chinese)
      Y. FAN (FAN YINGYING)*, Q. MA (MA QINGQING), F. YANG (YANG FENGMEI), W. ZHANG (ZHANG WEI), G. LUO (LUO GUIFA) (*National Med. Prod. Admin. Key Lab. for Qual. Contr. of Trad. Chinese Med. & Tibet. Med., Qianghai Prov. Key Lab. for Modern. of Trad. Chinese Med. & Tibet. Med., Qinghai Prov. Drug Insp. & Test. Inst., Xining 810016, China,

      J. Chinese Trad. Patent Med. 44 (6), 1770-1772 (2022). Ganqingqinglan (Dracocephalum tanguticum Maxim.) is a traditional Tibetan medicinal plant, containing flavonoids, terpenes, polysaccharides. It has antihypoxic, antibacterial, antiviral, and hypoglycemic effects and is used as the main component in the traditional Tibetan medicine for the treatment of hepatitis and gastritis. For quality control, TLC of the plant extracts on silica gel (1) for oleanic acid, with toluene - ethyl acetate - glacial acetic acid 28:8:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are clearly visualized, evaluation under UV 366 nm; (2) for chlorogenic acid, with toluene - methyl acetate - propanone - methanol - methyl acid 30:5:5:20:1, evaluation under UV 366 nm. The method was applied for the analysis of 18 batches of real life samples and proved to be simple, specific, reproducible, robust and well suitable for the purpose.

      Classification: 32e
      131 029
      Glehnia littoralis Fr. Schmidtex Miq.: A systematic review on ethnopharmacology, chemical composition, pharmacology and quality control
      S. LI (Li Shiyang), N. XU (Xu Nan), Q. FANG (Fang Qinqin), X. CHENG (Cheng Xuemei), J. CHEN (Chen Jiamei), P. LIU (Liu Ping), L. LI (Li Li), C. WANG (Wang Chanhong)*, W. LIU (Liu Wei) (*Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Department of pharmacy, The SATCM Third Grade Laboratory of Traditional Chinese Medicine Preparations, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China,

      J. Ethnopharmacol. 317, 116831 (2023). Review of traditional uses, ethnopharmacology, chemical composition, pharmacological activities, and quality control of Glehnia littoralis. Methods and representative sample preparation procedures for analysis of markers in G. littoralis were described, including HPTLC for the analysis of markers such as falcarindiol, scopoletin, umbelliferone and isoimperatorin.


      Classification: 1b
      131 030
      Ameliorative effect of traditional polyherbal formulation on TNF-α, IL-1β and Caspase-3 expression in kidneys of wistar rats against sodium fluoride induced oxidative stress
      M. KHAN, P. BASIST, S. ZAHIRUDDIN, N. PENUMALLU, S. AHMAD (*Centre of Excellence in Unani Medicine (Pharmacognosy & Pharmacology), Jamia Hamdard, New Delhi,110062, India,

      J. Ethnopharmacol. 318, 116900 (2024). HPTLC of gallic acid (1), quercetin (2), and ferulic acid (3) in the polyherbal formulation Sharbat-bazoori Motadil (SBM) on silica gel with toluene - ethyl acetate - formic acid 6:3:1. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 200-4000 ng/zone for (1) to (3). Intermediate precisions were below 3 % (n=3). LOD and LOQ were 18 and 54 ng/zone for (1), 17 and 52 ng/zone for (2) and 15 and 46 ng/zone for (3), respectively. Recovery was between 96.8 and 97.4 % for (1), 100.8 and 101.7 % for (2) and 101.6 and 102.6 % for (3).



      Classification: 7
      131 011
      Standardization of pharmacognostic and preliminary phytochemical parameters of Trianthema portulacastrum Linn. root: A halophytic plant
      M.P.V. VIKRAM SINGH, V. KUMAR PRAJAPATI* (*Department of Botany, Shri Jai Narain Mishra P.G. College, Lucknow, Uttar Pradesh, India;

      J Pharmacogn Phytochem, 12(1), 159-167 (2023). TLC of methanolic Soxhlet extracts on silica gel with toluene – ethyl acetate – formic acid 16:4:1. Visualization under UV 254 nm and 366 nm. Two bands only (hRF values 10 and 45), observed with densitometric scanning at UV 366 nm, were present in Trianthema portulacastrum roots (Aizoaceae), whereas a different profile was observed with Boerhavia diffusa roots (Nyctaginaceae), which is sometimes substituted for T. portulacastrum.

      Classification: 32e
      131 009
      Detection of coumarin derivatives of Viola odorata cultivated in Iraq
      Zainab A. ALI*, I. SALEH, W.M.K. ALANI (*College of Pharmacy, Mustansiriah University, Baghdad, Iraq;

      J Pharm Bioallied Sci 15(Suppl.2), S948-S951 (2023). Sample was the ethyl acetate fraction of an ethanolic extract of Viola odorata aerial parts (Violaceae). Standards were coumarinic compounds: esculetin (1) and umbelliferone (2). TLC and HPTLC on silica gel with toluene – ethyl acetate – formic acid 5:4:1. Visualization under UV 254 nm and 366 nm; densitometric scanning at 366 nm. Both (1) and (2) were found in the extract (hRF values 30 and 53, respectively, in TLC). Alternative mobile phases were also tested (TLC only): toluene – ethyl acetate 1:1 (hRF values 47 and 68) and chloroform – methanol 97:3 (hRF values 20 and 41).

      Classification: 8b, 32e
      131 008
      Structural characterization and in vitro biological exploration of phytoconstituents isolated from a chloroform extract of Rauvolfia vomitoria (Apocynaceae) root bark from Côte d’Ivoire
      (*Laboratoire de Chimie Bio-Organique et de Substances Naturelles (LCBOSN), Université Nangui Abrogoua, Abidjan, Côte d’Ivoire;

       J. Pharmacogn. Phytochem. 12(1), 6-14 (2023). TLC silica gel layers were used to monitor the purification through column chromatography (CC) of a chloroform fraction of the methanolic root bark extract of Rauvolfia vomitoria (Apocynaceae). Mobile phases were petroleum ether – ethyl acetate 4:1 (MP1), dichloromethane – methanol 20:1 (MP2), and dichloromethane – methanol 15:1 (MP3). Visualization under UV 254 nm. Preparative TLC on thicker silica gel was performed on two subfractions: (A) with dichloromethane – methanol 100:7 for the isolation of the methyl esters of eudesmic acid and of trimethoxycinnamic acid (hRF values 35 and 28, respectively, in MP1); (B) with MP2 for the isolation of an indole alkaloid: kumujan B (= 1-carbomethoxy-β-carboline, hRF value 40 in MP2). Other indole alkaloids were isolated through CC: ajmaline, mauensine and reserpine (hRF values 35, 13 and 47, respectively, in MP3).

      Classification: 4d, 7, 9, 22, 32e
      131 072
      Development of a validated high‑performance thin‑layer chromatography method for the standardization of an Ayurvedic formulation using berberine and ursolic acid
      V. KHANVILKAR*, S. MANDLE, P. HANDE (*Department of Quality Assurance, Bharati Vidyapeeth’s College of Pharmacy, Sector 8, C.B.D. Belapur, Navi Mumbai 400614, India,

      J. Planar Chromatogr. 35, 603-608 (2022). HPTLC of berberine (1) and ursolic acid (2) in an Ayurvedic formulation on silica gel with chloroform - acetone - formic acid 12:7:1. Quantitative determination by absorbance measurement at 330 nm. The hRF values for (1) and (2) were 46 and 68, respectively. Linearity was between 200 and 1000 ng/zone for (1) and 500 and 2500 ng/zone for (2). Intermediate precisions were below 2 % (n=9). The LOD and LOQ were 91 and 175 ng/zone for (1) and 153 and 465 ng/zone for (2), respectively. Recovery was in the range of 98 and 102 % for (1) and (2).

      Classification: 14, 22