Pharmacogn. Mag. 19, 917-925 (2023). HPTLC profiling of Guggulutiktam Kashayam on silica gel with toluene - ethyl acetate - methanol 7:3:1. Detection by spraying with anisaldehyde sulphuric acid reagent. Qualitative identification under UV light at 254 and 366 nm. 

Chinese J. Ethnomed. & Ethnopharm. 32 (22), 42-46 (2023). Sophora alopecuroides is a medicinal plant growing in Northern China, containing alkaloids, flavonoids, volatile oils and amino acids. It has antipyretic detoxification, antibacterial and anti-inflammatory, as well as analgesic effects, and is the main effective ingredient in some TCM mixtures widely used in clinical practice. This research team developed a medicinal gel, Kudouzi Ningjiaoji gel, which has good clinical results in the treatment of acne, pustules and other diseases. For quality control, TLC of the extracts of Kudouzi Ningjiaoji gel on silica gel with dichloromethane – methanol – ammonia water 50:6:1. Detection in daylight after spraying with a solution of bismuth potassium basic nitrate 0.85 g, glacial acetic acid 10 mL, water 40 mL and potassium iodide 2 g. Identification by comparing of sample fingerprints with those standards sophoridine and matrine.

J. Ethnopharmacol. 320, 117404 (2024). HPTLC of lupeol and stigmasterol in the stem bark of Ficus benghalensis, Ficus glomerata, Ficus religiosa, Ficus infectoria, and Albizia lebbeck on silica gel with toluene - ethyl acetate - methanol - formic acid 38:5:5:20:1. Detection by spraying with anisaldehyde sulfuric acid. Qualitative identification under UV light at 525 nm.

J. Ethnopharmacol. 320, 117387 (2024). Review of the anti-infective properties of Pluchea indica, including HPTLC methods for the analysis of molecules present in different parts of the plant, such as thalictoside.

J. Chinese Pharm. 34 (1), 52-56 (2023). Inonotus obliquus is a new source of fungal medicine, containing polysaccharides, polyphenols, triterpenoids, sterols and folic acid derivatives, with various pharmacological effects such as anti-tumor, hypoglycemic, antioxidant, and antiviral. For identification, TLC of its extracts on silica gel, A) for thrombotic acid, with chloroform – toluene – ethyl acetate – methanol 10:4:1:1; and B) for inotodiol, with dichloromethane – toluene – ethyl acetate – methanol 100:40:3:3. Detection for both by spraying with 5 % phosphomolybdate in ethanol and heating at 105°C until the zones are visible in daylight. Identification by fingerprint comparison with the standard drug Inonotus obliquus and the standards thrombotic acid and inotodiol. Chemical pattern recognition analysis was applied on resulting TLC and HPLC data (such as, cluster analysis, principal component analysis, orthozonal least squares-discriminant analysis) to evaluate the quality of the medicine.

Chinese J. Veter. Drug 57 (6), 52-57 (2023). Wupi Koufuye is a multi-ingredient TCM veterinary drug clinically used for the prophylaxis and treatment of gout in animals. For quality control, TLC on silica gel A) for White Mulberry root bark, developed twice with cyclohexane – ethyl acetate – methanol – concentrated ammonia solution 75:75:15:2, detection at UV 366 nm; B) for Pericarpium citri reticulatae, developed with trichloromethane - methanol – concentrated ammonia solution 100:30:3, detection by spraying with 0.5 % ninhydrin in ethanol and heating at 105 °C until the zones are visible in daylight; C) for Zingiber officinale Rosc., with n-hexane, detection by spraying with 0.5 % vanilin in sulfuric acid – ethanol 4:1 followed by heating at 105°C until the zones are visible in daylight.

J. China Pharm. 32 (6), 71-74 (2023). Shegan Kangbingdu Koufuye is a multi-ingredient TCM oral liquid with antiviral effects. For quality control, TLC on silica gel A) for Blackberrylily rhizome, with chloroform – methanol – glacial acetic acid 200:30:2, detection by spraying with 2 % FeCl3 in ethanol, evaluation in fluorescence; B) Radix Bupleuri,  with chloroform – methanol – water 13:7:2, detection by spraying with 2 % 4-dimethylaminobenzaldehyde in 40 % sulfuric acid solution and heating at 60 °C until the zones are visible at UV 366 nm; C) for Folium Isatidis and Radix Isatidis, with cyclohexane - chloroform – propanone 5:4:2, detection in fluorescence light; D) for Capillary Wormwood Herb, Honeysuckle and Dandelion, with toluene – ethyl formate – formic acid 6:4:1, detection in fluorescence light.

Chinese J. Pharm. Research 42 (2), 98-104 (2023). Ganoderma lucidum is the dry fruiting body of fungus, a medicinal plant, having the effect of relieving asthma and relieving nerves. Ganoderma lucidum is widely distributed in China and mainly concentrated in Zhejiang, Anhui, Shandong, Jilin, and Fujian. In this study, the HPTLC method for quality detection and origin traceability was established towards the medicinal material produced in Fujian and other areas. HPTLC of the ethanol extracts of the sample materials on silica gel with chloroform – acetonitrile – methanol – formic acid 130:15:2:2, three times to distances of 3 cm, 6 cm, 8 cm. Detection by spraying with 3 % sulfuric acid in ethyl acetate and heating at 105°C until the bands are clearly visualized, evaluation at UV 366 nm. The bands on the thin layer chromatogram were identified by fingerprint comparison using standard samples, and cluster analysis for the data of samples from different producing areas was performed using digital characterization. As results, the HPTLC analysis detected 17 distinct bands and among them, 11 components were identified, e.g. ganoderenic acid C (hRf 0.31), ganoderic acid C2 (hRf 33), ganoderic acid l (hRf 35), ganoderic acid G (hRf 41), ganoderic acid A (hRf 44), ganoderic acid B (hRf 46), ganolactone B (hRf 53), 3β,7β,15β-trihydroxy-11,15-dihydroxy-lanostane-8-alkene-24→20 lactone (hRf 56), ganoderenic acid D (hRf 61), ganoderic acid D (hRf 63) and 20 (21)-dehydrolucidenic acid A (hRf 65). A total of 10 HPTLC bands were identified in Fujian Ganoderma lucidum, among which ganoderic acid C2, ganoderic acid D and two unknown component bands (hRf 14 and 17) were unique and could be used as markers of Fujian Ganoderma lucidum. Thus samples of Fujian Ganoderma lucidum can be differentiated from those of other areas, when the Euclidean distance is 25 in Euclidean cluster analysis.