Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      124 037
      Comparison between HPLC and HPTLC densitometry for the determination of spinosin from Ziziphus jujuba Mill. fruit extracts
      Z. SOBHANI, S. EMAMI, O. RAJABI* (*Department of Pharmaceutical Control, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran,

      J. Liq. Chromatogr. Relat. Technol. 42, 563-569 (2019). HPTLC of spinosin in the fruits of Ziziphus jujuba on silica gel with ethyl acetate - dichloromethane - methanol - water 18:10:15:5. Quantitative determination by absorbance measurement at 334 nm. The hRF value for spinosin was 38. Linearity was between 10 and 120 ng/mL. Intermediate precision was below 2 % (n=6). The LOD and LOQ were 12 and 35 ng/mL, respectively. Recovery rate was between 98.7 and 101.3 %. The HPTLC method provided similar reproducibility, accuracy and selectivity for the quantitative determination of spinosin compared with a HPLC method.

      Classification: 8a
      124 047
      Lipophilicity estimation of anti-proliferative and anti-inflammatory 6-substituted 9-fluoroquino[3,2-b]benzo[1,4]thiazines
      Malgorzata JELEN, K. PLUTA, B. MORAK-MLODAWSKA* (Department of Organic Chemistry, School of Pharmacy with the Division of Laboratory Medicine, The Medical University of Silesia, Sosnowiec, Poland,

      J. Liq. Chromatogr. Relat. Technol. 42, 563-569 (2019). TLC of 17 new anti-proliferative and antiinflammatory tetracyclic 6-substituted 9-fluoroquinobenzothiazines on RP-18 with acetone - aqueous TRIS (tris(hydroxymethyl)-aminomethane) buffer pH 7.4 (ionic strength 0.2 M), where the concentration of acetone was from 50 to 85 % in 5 % increments. Lipophilicity parameters were determined (RM0 and logPTLC) and compared with computationally calculated lipophilic parameters. Relation of lipophilicity with predicted and experimental biological activities was discussed.

      Classification: 2c
      124 054
      (Determination of ginseng saponin in Panax notoginseng powders by thin-layer chromatography) (Chinese)
      F. HE (He Fulong), Y. ZHENG (Zheng Yanping)*, J. REN (Ren Juan), J. JIN (Jin junjie), F. BAI (Bai Faping), B. CAO (Cai Baochang) (*Nanjing Haichang Chinese Med. Group Co. Ltd., Nanjing 210061, China)

      J. of Modern Trad. Chinese Med. 20 (8), 975-978 (2018). Panax notoginseng is a traditional Chinese medicinal herb which activates blood circulation, anti-platelet aggregation and anti-thrombosis. TLC for quality control of ginsenoside Rb1 (1), notoginsenoside R1 (2) and ginsenoside Rg1 (3) on silica gel with the lower phase of chloroform – methanol – water 13:7:2 (after standing for 12 h at below 10˚C). Detection by spraying with 10% sulfuric acid in ethanol and heating at 110 ˚C until the zones are visible, evaluation under UV 365 nm. Quantification by densitometric absorption measurement at 510 nm. Validation by investigation of the linearity ranges of 0.5-5.0 µg/zone (r=0.998) for (1), 0.5-5.01 µg/zone (r=0.999) for (2) and 0.5-4.9 µg/zone (r=0.998) for (3). The plate-to-plate precision % RSD (n=12) was 1.5 %, 1.1 % and 1.9 % for (1) to (3). Recovery from standard sample addition was 96.4 % (%RSD 1.4 %, n=6) for (1), 96.9 % (%RSD 0.9 %, n=6) for (2), and 98.9% (%RSD 1.7 %, n=6) for (3).

      Classification: 32e
      124 057
      Antibacterial potential of the Cistus incanus L. phenolics as studied with use of thin-layer chromatography combined with direct bioautography and in situ hydrolysis
      Ágnes M. MÓRICZ*, D. SZEREMETA, M. KNAS, E. DŁUGOSZ, P.G. OTT, T. KOWALSKA, M. SAJEWICZ (*Dep. of Pathophysiology, Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman O. Street 15, 1022 Budapest, Hungary)

      J. of Chromatogr. A 1534, 170-178 (2018). HPTLC of flavonoid aglycones in eleven commercial Cistus incanus herbal teas using three independent methods (multi-development on amino phase and two two-dimensional developments on silica gel phase). HPTLC on silica gel with chloroform - methanol - ethyl acetate 15:3:2. Detection at UV 254 and 366 nm and by derivatization with aluminium chloride (1 % methanolic solution), ferric chloride (0.5 g FeCl3 in 2.5 mL water and 47.5 mL ethanol), NP-PEG (0.5 % methanolic NP solution, and after drying, 5 % ethanolic PEG solution), PABA (0.5 g PABA in 18 mL glacial acetic acid diluted with 20 mL water, plus 1 mL o-phosphoric acid and 60 mL acetone), or DPA reagents (1 g aniline and 1 g diphenylamine in 100 mL acetone and 10 mL o-phosphoric acid, heated for 5 min at 110°C). Confirmation of the presence of glucose by HPTLC on amino phase with in situ acid hydrolysis: incubation in HCl vapor followed by heating at 100°C, pre-development with acetonitrile, drying, development with acetonitrile - water 7:3, heating for 20 min at 170°C and dipping in paraffin - n-hexane 1:2 follwed by drying. TLC-direct bioautography by immersion of the developed and dried plates in a bacterial cell suspension of either Bacillus subtilis or Aliivirio fischeri strains. Analysis of the compounds isolated from the bioactive zones by HPLC-diode array detector (DAD)-electrospray ionization (ESI)-MS. The presented TLC/HPTLC platform allowed identification of the antibacterial components apigenin, kaempferide, cis- and trans-tiliroside, and the isomers of the p-coumaric acid-conjugated tiliroside,  all of them inhibiting both B. subtilis and A. fischeri.

      Classification: 32e
      124 061
      Tempeh & soybean seed coat: the alternative sources of trans-resveratrol as neuroprotective agents
      Yulia IRNIDAYANTI*, D. SUTIONO (*Departement of Biology, Faculty of Mathematics and Science, Universitas Negeri Jakarta, Hasyim Asyarie Building, Rawamangun muka, Indonesia,

      Int. J. Morphol. 37, 1164-1171 (2019). HPTLC of transresveratrol in traditional fermented soybean seed coat on silica gel with chloroform - ethyl acetate - formic acid 25:10:1. Qualitative identification under UV light at 254 and 366 nm. The hRF value for transresveratrol was 64.

      Classification: 7
      124 056
      Bioprofiling of Salvia miltiorrhiza via planar chromatography linked to (bio)assays, high resolution mass spectrometry and nuclear magnetic resonance spectroscopy
      E. AZADNIYA, Gertrud E. MORLOCK* (*Chair of Food Science, Institute of Nutritional Science, Interdisciplinary Research Center (IFZ), Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany,

      J. of Chromatogr. A 1533, 180-192 (2018). HPTLC-UV/Vis/FLD-(bio)assay-HRMS of polar (phenolics) and nonpolar (tanshinones) extracts of Salvia miltiorrhiza Bunge root (Danshen), followed by streamlined scale-up to preparative layer chromatography with 1H-NMR. For phenolics, HPTLC on silica gel first with toluene - chloroform - ethyl acetate - methanol - formic acid 4:6:8:1:1 and second development with petroleum ether - cyclohexane - ethyl acetate 25:14:11. Confirmation of the acetylcholinesterase inhibitors salvianolic acid B (1), lithiospermic acid (2), rosmarinic acid (3), cryptotanshinone (4) and 15,16-dihydrotanshinone I (5). In the polar extracts, compounds (1), (2) and (3) exhibited free radical scavenging activity in the 2,2-diphenyl-1-picrylhydrazyl assay (DPPH* radical reagent), (4) and (5) were active against Bacillus subtilis and Aliivibrio fischeri (LOD of 12 ng/zone for (4), and 5 ng/zone for (5). For the first time, the unidentified, most active zone of the nonpolar Danshen extract was identified as a co-eluting zone of 1,2-dihydrotanshinone and methylenetanshinquinone 2:1.

      Classification: 32e
      124 019
      Thin-layer chromatographic enantioresolution of (RS)-ketorolac using L-amino acids as chiral additive in stationary phase
      M. SINGH*, R. BHUSHAN (*Department of Chemistry, Lovely Professional University, Phagwara 144411, Punjab, India,

      J. Planar Chromatogr. 32, 475-479 (2019). TLC of enantiomers of (RS)-ketorolac on home-made silica gel plates impregnated with L-Tryp (1), L-Val (2), L-Met (3) or L-His (4) with acetonitrile - methanol - water - chloroform 9:5:2:4 for (1), acetonitrile - methanol - water - dichloromethane 6:2:1:1 for (2), acetonitrile - methanol - water - chloroform 5:3:1:1 for (3) and acetonitrile - methanol - water - chloroform 10:4:1:5 for (4), respectively. Detection by exposure to iodine vapors. The hRF values for (S)-(‒)- and (R)-(+)-ketorolac were 36 and 81 for (1), 30 and 79 for (2), 30 and 87 for (3) and 42 and 85 for (4), respectively. LOD was 0.4 μg/mL.

      Classification: 38
      124 062
      Rapid isolation of a dipeptidyl peptidase IV inhibitor from Fritillaria cirrhosa by thin-layer chromatography–bioautography and mass spectrometry-directed autopurification system
      L. GU, T. TIAN, L. XIA, G. CHOU*, Z. WANG (*Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China,

      J. Planar Chromatogr. 32, 447-451 (2019). HPTLC of Fritillaria cirrhosa on silica gel with ethyl acetate - methanol - ammonia solution - water 180:20:10:1. Bioautography by dipping into a 0.15 mg/mL solution of substrate Gly-Pro-p-nitroanilide hydrochloride in 50 % of ethanol, followed by ethanol removal in the hood and dipping into a 10 U/L DPP IV enzyme solution in TrisHCl buffer (pH 8.2, 70 mM), followed by incubation at 37°C for 40 min. Detection by dipping into a solution of 0.5 % sodium nitrite in 1.2 M hydrochloric acid, followed by drying slightly for 5 min and dipping into 0.05 % N-(1-naphthyl)ethylenediamine dihydrochloride solution. Further analysis by mass spectrometry using a TLC interface. The hRF value for the dipeptidyl peptidase IV inhibitor was 58.

      Classification: 4e, 8b