Front. Nutr. 9, 887992 (2022). HPTLC of 13 mogrosides, 1 flavonoid, and 3 sugars in monk fruit products on silica gel with n-butanol - water - ethanol - acetic acid 70:10:10:2. Detection by spraying with 10 % sulfuric acid in ethanol solution, followed by heating at 105 °C for 10 min. Orthogonal partial least square-discriminant analysis (OPLS-DA) was subsequently conducted allowing the identification mogroside V, 11-oxo-mogroside V, isomogroside V, mogroside IV and sucrose to be the characteristic compounds to distinguish the two types of monk fruits.

Food Chem. DOI: 10.1111/1750-3841.16708 (2023). HPTLC of phenylpropanoids in the leaves of Ginkgo biloba on silica gel with dichloromethane - ethyl acetate 1:1. Detection by spraying with 10 % sulfuric acid ethanol solution, followed by heating. Qualitative identification under UV light at 366 nm. Further analysis by nuclear magnetic resonance. The method allowed the identification of phenylpropanoids suitable for controlling foodborne pathogens.

Food Chem. 88, 2902-2918 (2023). HPTLC of high free fatty acid, monoacylglycerols, diacylglycerols and triacylglycerol on silica gel with hexane – diethyl ether - acetic acid 50:50:1. 

Anal. Bioanal. Chem. https://doi.org/10.1007/s00216-023-04605-x (2023). HPTLC of 60 pesticides (1), six plant protection products (2), tomato (3) and grape and wine samples (4) on silica gel with n-hexane - ethyl acetate 5:1, n-hexane - toluene - ethyl acetate 4:1:1 for (2), n-hexane - toluene - ethyl acetate 5:1:1 for (3) and n-hexane - ethyl acetate 5:1 for (4). Documentation in fluorescence mode at 366 nm. pYES bioassay application by dipping into a citrate phosphate buffer, followed by drying and dipping into yeast cell suspension, followed by incubation at 30 °C for 3 h. After drying, the chromatogram was immersed into a MUG solution, followed by incubation at 37 °C for 1 h. Detection at FLD 366 nm/ > 400 nm.

Anal. Bioanal. Chem. https://doi.org/10.1007/s00216-023-04820-6 (2023). HPTLC of bisphenols in complex mixtures such as six tin can migrates (1), five thermal papers (2), and eleven botanicals (3) on silica gel with toluene - ethyl acetate 6:1 for (1), n-hexane - ethanol - ethyl acetate 40:3:3 for (2) and ethyl acetate - toluene - methanol - water 16:4:3:2 for (3). Documentation under white light illumination, UV 254 nm and FLD 366 nm. Biological detection by spraying with Arxula adeninivorans yeast cell suspension, followed by incubation at 37 °C for 2 h. After drying in a cold stream, the plate was sprayed with the luciferin substrate solution and bioluminiscence was recorded.  

Biochem. Biophys. Res. Commun. 667, 146-152 (2023). HPTLC of intracellular lipids in sebocytes treated with calcium for 24 h and incubated with medium containing 2 µCi of [1-¹⁴C] on silica gel with hexane - ethyl acetate 6:1. Detection by autoradiography. The method allowed the identification of squalene, trygliceride and cholesterol.     

J. Sep. Sci. 46, 2300059 (2023). HPTLC of iridoid and iridoid glycoside in Premna fulva on silica gel with ethyl acetate - n-butanol - water 3:1:4. Detection by spraying with 10 % sulfuric acid - ethanol chromogenic agent, followed by heating. The system showed a hRF values from 30 and 70. The method was applied for candidate solvent-system prediction with a generally useful estimate of the solvent systems (GUESS). Further analysis by high-speed counter-current chromatography.

J. Sep. Sci. 46, 2200921 (2023). Review of the techniques for the analysis of sodium-glucose linked transporter-2 (SGLT-2) inhibitors, including HPTLC for the determination of canagliflozin, dapagliflozin and empagliflozin.