J. of Guangzhou Chem. Industry. 40 (24), 122-123 (2012). Cleavers (Galium aparine) is a herb used as a key component drug in various TCM formulations for the treatment of tumours, dysentery, mastitis, urinary tract infections, gallbladder diseases etc. For quality control and to guarantee the medication safety of relevant formulations, TLC of chlorogenic acid, the main active ingredient, on silica gel with ethyl acetate – formic acid – water 14:1:1, detection 1) by viewing under UV 366 nm; 2) by spraying with 2 % ferric chloride in ethanol and heating mildly then viewing in daylight; 3) by densitometry at 585 nm.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (4), 829-830 (2011). Gongbaokang suppository is a traditional Chinese recipe for curing cervicitis, preventing pathological changes which may cause cervical carcinoma. Description of a method for the quality control. TLC of the extracts of the medicine 1) for Sophora flavescens, on silica gel with benzene – acetone – ethyl acetate – ammonia 10:15:20:1, detection by spraying with 5 % potassium iodobismuthate solution and viewing under daylight; 2) for Fructus Cnidii, on silica gel with toluene – ethyl acetate – n-hexane 3:3:2, detection under UV 366 nm; 3) for borneol, on silica gel with benzene – acetone 9:1, detection by spraying with 1 % vanillin in ethanol – sulfuric acid 4:1 and heating at 105 °C, viewing under UV 366 nm.

J. of Inform. on Trad. Chinese Med. 29 (6), 96-98 (2012). Fangji Fuling Tang decocted extract is a herbal TCM preparation for the treatment of chronic nephritis edema, cardiac edema, etc. For quality control the method of the Chinese Pharmacopeia is improved. TLC on silica gel 1) for Radix Stephaniae Tetrandrae and the standards (+)-tetrandrine, hanfangichin B, and fangchinoline, with chloroform – methanol – acetone 6:1:1, detection by spraying with 5 % potassium iodobismuthate in hydrochloric acid – water 1:200 and viewing in daylight; 2) for Radix Astragali seu Hedysari and the standard astragaloside A, with the lower phase of chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating mildly, viewing in daylight; 3) for Radix Glycyrrhizae, with the lower phase of ethyl acetate – formic acid – glacial acetic acid - water 15:1:1:1, detection under UV 366 nm. Quantification of (+)-tetrandrine, hanfangichin B, and fangchinoline by HPLC.

J. Planar Chromatogr. 26, 452-454 (2013). Optimization of temperature and duration required for detection of terpene trilactones. TLC of ginkgolides A, B and C and bilobalide on silica gel with ethyl acetate - hexane 9:1. Detection by spraying with acetic anhydride followed by heating at 80, 100, 120 and 140 ºC. The formation of alpha, beta-unsaturated gamma-lactone from ginkgolides was detected even at low temperatures for a respective heating time.

Chinese J. of Northern Pharmacy 10 (10), 9-10 (2013). Keke Diwan drop pills are a herbal TCM preparation for the treatment of cough, dyspnea and shortness of breath. For quality control, TLC on silica gel 1) for Ephedra sinica Stapf and the standard ephedrine hydrochloride, with chloroform – methanol – ammonia 100:20:1, detection by spraying with 0.3 % ninhydrin in n-butanol – glacial acetic acid 19:1 and heating at 105 °C, viewing in daylight; 2) for Pericarpium papaveris, with toluene – acetone – ethanol – concentrated ammonia 20:20:3:1, detection under UV 366 nm; 3) for Glycyrrhiza uralensis Fisch., with benzene – chloroform – methanol – glacial acetic acid 40:90:15:1 with preconditioning for 10 min, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.

J. of Modern Trad. Chinese Med. 15 (4), 320-323 (2013). Leifengshi Jiaonang capsules are a herbal TCM preparation for the treatment of rheumatism, ankylosing spondylitis, and lupus erythematosus. For quality control, TLC on silica gel 1) for Astragalus membranaceus (Fisch.) Bunge and the standard astragaloside A, with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol, heating at 105 °C and viewing in daylight and under UV 366 nm; 2) for Angelica sinensis, with petroleum ether (60-90 °C ) – diethyl ether – acetic acid 20:20:1, detection under UV 366 nm 3) for Epimedium sagittatum, with ethyl acetate – formic acid – butanone – water 10:1:1:1, detection under UV 366 nm. Quantification of ferulic acid by HPLC.

Chinese J. of Inform. on TCM 19 (7), 46-48 (2012). Tiaojing Huayu Wan pills are a herbal TCM preparation for treatment of menstrual disorders, menstrual abdominal pain, amenorrhea caused by vital energy stagnation and blood stasis. For quality control, TLC on silica gel 1) for Rhizoma Cyperi, with benzene – ethyl acetate – glacial acetic acid 92:5:5, detection under UV 254 nm; 2) for Radix Angelicae Sinensis, Rhizoma Ligustici Chuanxiong, and the standard ferulic acid with benzene – ethyl acetate – formic acid 40:10:1, detection under UV 366 nm. Quantification of paeoniflorin by HPLC.

J. Liq. Chromatogr. Relat. Technol. 37, 2021-2035 (2014). HPTLC of thirteen 5-arylidene-2,4-thiazolidinediones on RP-18 with one of six organic solvents: acetonitrile (with increasing acetonitrile content from 55% to 75%), methanol (60–90%), ethanol (40–70%), propanol (35–55%), acetone (55–75%), and dioxane (50–70%) with each modifier in increasing steps of 5%. A quantitative structure-retention relationship study allowed to investigate the retention behavior of these substances.