Phytochem. Anal. 35, 10.1002/pca.3297 (2023). HPTLC of aerial parts of Hypericum perforatum on silica gel with ethyl acetate - toluene - acetic acid - formic acid 7:3:1:1. Detection by spraying with 50 % sulfuric acid and vanillin, followed by heating and visualization under UV light at 254 and 366 nm. 

Phytochem. Anal. 35, 163-183 (2024). HPTLC of Cannabis sativa on silica gel with n-heptane - methyl tert-butyl ether - ethanol - formic acid 780:170:50:3. Detection by spraying with vanillin-sulfuric acid (VSA) reagent (5 % methanolic solutions), followed by heating at 120 °C for 3 min. Fast Blue solution (0.5 % in water - methanol - dichloromethane 2:5:3) was used for derivatisation as a selective stain for cannabinoids. A 0.1 M sodium hydroxide solution in methanol - water 9:1 was used to improve color development.

Trends Anal. Chem. 168, 117362 (2023). Review of functional and non-functional assays in the study of acetylcholinesterase (AChE) ligand discovery, including HPTLC for the assessing of AChE inhibition and for monitoring of ChE activity.

PLoS Pathogens 19(9), e1011636 (2023). Samples were total lipid extracts from Mycobacterium tuberculosis (Mycobacteriaceae) either wild-type (1), or a mutant strain knock-out for the gene of succinyl-transferase (sucT) (2), or addback bacteria obtained through plasmide transformation of the wild-type sucT gene into the mutant (3). Development on TLC silica gel layers with chloroform – methanol – water 65:25:4. Derivatization by spraying with cupric sulphate (A) or with α-naphthol (B), followed by heating above 100°C. The following lipids were detected with both reagents (as brown spots on blue background with (A) or as purple spots on yellowish background for (B)) in all strains, without significant quantitative variations: cardiolipin, and acylated phosphatidyl–myo-inositol mannosides.

PLoS Neglected Tropical Diseases 17(09), e0011646 (2023). Samples were extracts rich in sphingolipids obtained from Trypanosoma cruzi epimastigotes, or from Leishmania major promastigotes (Trypanosomatidae), or from Chlorocebus sp. kidney Vero cells (Cercopithecidae), all cell lines incubated 2h before the extractions with ceramide N-hexanoyl-D-erythro-sphingosine coupled to fluorescent NBD-amine group (NBD = nitrobenzoxadiazolyle). Dried extracts were resuspended in chloroform – methanol (1:1) before application on TLC silica gel layers. Development with chloroform – methanol – potassium chloride 0.25 % aqueous solution 11:9:2. Visualization under automated laser scanner. Three sphingolipids were detected due to the NBD fluorescent group: sphingomyelin (hRF 42) was present in Vero cells only (negative control), whereas the targeted inositol-phosphorylceramide (IPC, hRF 70), was present in both L. major (positive control) and T. cruzi wild-type. It was absent in T. cruzi cell lines knock-out (KO) for the IPC-synthase (IPCS) gene, but present again in the add-back cell-lines (obtained with plasmide transfection of the IPCS gene into KO cells). An unknown lipid (hRF 78) was detected in all T. cruzi samples.

PLoS ONE 18(11), e0294054 (2023). Sample was a pigment extracted with methanol from cell pellets of Serratia marcescens strain BRL41 (Yersiniaceae). TLC on silica gel with ethyl acetate. Detection under daylight. The pink pigment had the same hRF value (87) and colour as prodigiosin from standard strain ATCC-13880.

PLoS ONE 18(11), e0294775 (2023). Sample was an Azadirachta indica seed extract (Meliaceae). TLC on silica gel with different mobile phases: (1) diethyl ether – methanol 49:1; (2) diethyl ether – acetone 2:1; (3) isopropanol – n-hexane 11:9; (4) dichloromethane – methanol – acetic acid 95:5:1. After 30 min hot air drying,detection under UV light. The hRF values of azadirachtin A (a limonoid) were 75, 42, 44, 55, respectively. Mobile phase (1) was therefore chosen as solvent for purification of azadirachtin A and for its quantification by Fourier transform infrared spectroscopy (FTIR).

PLoS ONE 18(11), e0295012 (2023). TLC on silica gel to monitor the synthesis of 15 new camphene-based thiosemicarbazones produced by the reaction of camphene thiosemicarbazide either with benzaldehydes, or with acetophenones, or with one of the following molecules: benzophenone, cinnamic aldehyde, ethyl pyruvate, furaldehyde, menthone, pyrrole carboxaldehyde or thiophene-carboxaldehyde. Development with n-hexane – ethyl acetate 3:7 in the case of benzaldehydes, except vanillin; or 7:3 for the vanillin derivative and all others, followed by visualization of products with resublimated iodine. The aldehyde used for compound 15 is in fact vanillin.