Phytochemistry 48, 991-994 (1998). TLC of numerous methoxyflavone aglycones on polyamide DC6 with toluene - methyl ethyl ketone - methanol 4:3:3 and with toluene - petroleum ether - methyl ethyl ketone 60:26:7. Detection under UV 254 and 366 nm. Rf values and spectral properties of 17 different aglycones are given.

The effect of pH. J. Planar Chromatogr. 11, 64-69 (1998). Determination of the lipophilicity and specific hydrophobic surface area of 21 fused-ring nitrogen heterocycles by RPTLC and study of the effect of different mobile-phase additives such as acetic acid, sodium acetate, and lithium chloride on the retention. Regular retention behavior was observed for each solute, the RM value decreasing linearly with increasing concentration of acetonitrile in the mobile phase. RPTLC of 21 nitrogen containing heterocycles on silica gel impregnated with paraffin oil. Detection after drying at 105°C under UV and by exposure to iodine vapor.

by thin-layer chromatography.) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 20 (3), 16-17 (1998). TLC on silica gel with 1) benzene - ethyl acetate 19:1, 2) chloroform - ethyl acetate 9:1, 3) chloroform - methanol - water 10:10:1 and 30:10:1. Detection 1) by spraying with 2% vanillin in sulfuric acid - ethanol 1:4 and heating at 100°C for 5 min, 3) by spraying with 1% p-dimethylaminobenzaldehyde in sulfuric acid - ethanol 1:10 and heating at 100°C for 5 min, 4) under UV 365 nm. Identification by finger print techniques.

CBS 82, 9-11 (1999). HPTLC-AMD of glucose, maltooligosaccharides, anhydrosugars, transglycosidation products, fructose and its derivatives, maltulose, Amadori-compounds and Heyns-compounds on silica gel. Caramelization: isocratic 23-step gradient based on chloroform - methanol - 0.05 % aqueous boric acid 20:11:2 with 3 mm increments of the developing distance. Maillard-reaction: isocratic 3-step gradient chloroform - dichloromethane - methanol - water 35:15:35:6 over 25, 50, 75 mm developing distance. Detection by dipping in aniline-diphenylamine-phosphoric acid reagent, followed by heating at 120 °C for 10 min. Quantification by absorbance measurement at 385 nm.

Planta med. 68, 55-59 (2002). Analytical and preparative TLC of 3ß-angeloyloxy-8a-hydroxy-6ß-methoxyeremophil-7(11),9(10)-dien-8,12-olide, 2:3ß-angeloyloxy-6ß,8a-dihydroxyeremophil-7(11),9(10)dien-8,12-olide, 5:3,8-oxo-eremophila-6,9-dien-12-oic acid, 3:3ß-angeloyloxy-8a-hydroxy-6ß-ethoxyeremophil-7(11),9(10)-dien-8,12-olide, 4:3ß-angeloyloxy-8-oxo-eremophila-6,9-dien-12-oic acid on silica gel with chloroform - methanol 12:3:2 and dichloromethane - methanol 10:1.

Planta med. 68, 435-439 (2002). TLC of 10-hydroxy-N(a)-demethyl-19,20-dehydroraumacline on silica gel with ethyl acetate - methanol - water - ammonia 70:20:10:1, chloroform - methanol - water - ammonia 50:45:5:1 and 50:40:10:1. Detection under UV light at 254 nm and after spraying with cericammonium sulfate (CAS) reagent.

CBS 91, 5-7 (2003). HPTLC-AMD of benzodiazepines from serum on lichrospher silica gel with a 9-step gradient based on methanol and diisopropyl ether over 80 mm. Densitometric evaluation by absorbance measurement at 230 and 320 nm followed by spectra recording from 200 to 330 nm.

Indian Drugs 41 (1), 46-47, 2004. HPTLC on silica gel plates with n-propanol - formic acid - water 90:1:9. Rf of berberine was 0.39. Quantification by densitometry at 348 nm via linear regression in the range of 40-220 ng. Limit of quantification and limit of detection were found to be 30 ng and 10 ng, respectively. The herbal extract was found to contain 0.402 % w/w of berberine. The percentage of berberine in formulations I, II, III and IV were 0.0886 %, 0.0123 %, 0.0283 % and 0.0091 % w/w, respectively. The standardized method was found to be reproducible, accurate, precise and selective.