J. Chinese Modern Med. & Pharm. 20 (11), 68-69 (2013). Heidouliuyou Ruangao ointment is a herbal TCM for diminishing inflammation and promoting skin regeneration, and is prescribed clinically for the treatment of neurodermatitis, subacute and chronic dermatitis, chronic eczema, etc. For quality control, TLC of the sample extracts and the standards eucalyptol and borneol on silica gel with n-hexane – toluene – acetone 24:16:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible in daylight.

J. of China Pharm. 22 (3), 13-15 (2013). Gushangling Jiaonang capsule is a herbal TCM for post traumatic functional recovery, prescribed to treat muscle atrophy, bone hyperplasia, neck and lumbar diseases, nerve injuries etc. For quality control, HPTLC on silica gel 1) for Paeonia lactiflora Pall., with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 3 % vanillin in sulfuric acid – water 1:200 and heating mildly until the zones are visible in daylight; 2) for Angelica sinensis, with n-hexane – ethyl acetate – 9:1, detection under UV 366 nm; 3) Astragalus membranaceus (Fisch.) Bunge., with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight.

J. AOAC Int. 97, 1274-1281 (2014). TLC fingerprint for the optimization of accelerated solvent extraction conditions for the isolation of total polyphenolic compounds from common thyme (Thymus vulgaris), on silica gel with ethyl acetate – formic acid – acetic acid – water 100:11:11:13. Detection by spraying with 10 % methanol solution of sulfuric acid, followed by heating at 110 °C for 20 min. Quantitative determination by absorbance measurement between 320 and 700 nm.

Shanxi J. Trad. Chinese Med. 9, 43-46 (2013). Danggui Buxue Wan pill is TCM compound for the treatment of psoriasis (blood dryness type), chronic eczema, neurodermatitis, skin pruritus and other skin diseases. For the quality control, TLC on silica gel (1) for Angelica sinensis with cyclohexane – dichloromethane – ethyl acetate – formic acid 4:1:1:0.1, detection at UV 366 nm; (2) for Fallopia multiflora (Thunb.) Harald. with the upper phase of toluene – ethyl acetate – formic acid 20:2:1, detection at UV 366 nm; (3) for Paeonia lactiflora Pall. with chloroform – ethyl acetate – methanol - formic acid 45:5:10:0.2, detection by spraying with 5% vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the spots were visible. All were identified by fingerprint comparison with the standard (paeoniflorin or ferulic acid) and/or the standard ingredient drug.

J. Chinese Modern Med. & Pharm. 20 (31), 54-56 (2013). Paracetamol and chlorphenamine maleate granule are an anti cold medicine effective for cold induced nasal congestion, headache, sore throat, fever, etc. Artificial bezoar is one of the main ingredients and bilirubin is the key effective component in the medicine. For quality control, TLC of the extracts and bilirubin on silica gel with isooctane – ethyl acetate – glacial acetic acid 11:5:3, detection under white light. Quantitative determination of bilirubin by UV spectrophotometry. Demonstration by applying to real life samples from three pharmaceutical factories indicated that the method was suited for the quality control of the medicine.

Microbiol. Res. 169, 794-802 (2014). Preparative TLC of a biosurfactant-like compound secreted by Pseudomonas synxantha on silica gel with dichloromethane - methanol 9:1. Detection by iodine vapor. The band with the highest hRF value showed antibacterial activity against M. smegmatis (previously confirmed by spot assay) .

J. Planar Chromatogr. 28, 162-166 (2015). HPTLC of (1) sucrose, (2) glucose, (3) fructose, (4) arabinose, (5) cellobiose, (6) maltose, (7) mannose, (8) melezitose, (9) melibiose, (10) raffinose, (11) rhamnose, (12) stachyose, (13) trehalose and (14) xylose on silica gel with n-butanol - i-propanol - boric acid solution - formic acid 50:130:55:1 to a migration distance of 60 mm. Detection under white light after immersion into aniline diphenylamine o-phosphoric acid reagent and heating at 110 °C for 10 min. Qualitative detections were performed under white light and by absorption measurement at 380 nm. A total of 131 samples were analyzed with regard to their individual sugar composition and classified according to their sugar ratios.

J. Ethnopharmacol. 174, 178-186 (2015). HPTLC of magnolol (1) and honokiol (2) in the cortex of Magnolia officinalis and aristolochic acid I (3) and II (4) in the radix of Aristolochia baetica on silica gel with methanol - ethyl acetate - toluene 1:2:30 for (1) and formic acid - water - ethyl acetate - toluene 1:1:10:20 for (2). Detection of (1) and (2) by spraying with vanillin reagent, followed by heating at 110 °C for 5 min. Detection of (3) and (4) by spraying with stannous chloride 100 g/L in diluted hydrochloric acid, followed by heating at 100 °C for 1 min. Identification under UV light at 365 nm for (3) and (4). The hRF values for (1) to (4) were 40, 50, 46 and 54, respectively.