Application Notes

    HPTLC is the method of choice for many analytical tasks and a broad range of Application Notes is available for download. The Laboratory HPTLC develops and validates HPTLC methods for herbal drugs, food, cosmetics, forensics, and other application fields. By following these methods, using the recommended instruments and software, reproducible results are guaranteed. A standardized methodology according to the International Association for the Advancement of HPTLC is followed.

ID
Method
A-145.1
Detection of phospholipids by HPTLC – alternative method
A-144.1
Identification and quantification of nerve agents RVX, VX, GD, GA, GF and GB by HPTLC, and methodology for a large-scale untargeted screening
A-143.1
Identification and quantification of blistering agents and irritants HD, TTA, CS, CN, CA and CB by HPTLC
A-142.1
Identification and quantification of arsenics agents L, DA and DM by HPTLC
A-141.1
Identification of essential oils – Transfer of method from HPTLC to HPTLC PRO
A-140.1
Identification of Angelica species – Transfer of method from HPTLC to HPTLC PRO
A-139.1
Methodology for the identification and quantification of human milk oligosaccharides (HMO) by HPTLC
A-138.1
Analysis and quality control of lipid-based formulations, e.g. mRNA vaccines, by HPTLC
A-137.1
Identity Testing of Sorbitol – Transfer of Method from TLC to HPTLC/HPTLC PRO, and Expansion of Scope
A-136.1 PRO
Quantification of carbohydrates in various matrices
A-134.1
Identification of oleander (Nerium oleander L.) and detection of oleandrin, a toxic cardiac glycoside
A-133.1
Quality control of lateral flow test strips by scanning densitometry analysis (e.g. COVID-19)
A-132.1
Quantification of piperine in black pepper fruit (Piper nigrum L.) and test for minimum content (MCT) of piperine (> 3% Ph. Eur.)
A-131.1
Limit test for adulteration of black pepper fruit (Piper nigrum L.) with papaya seed (Carica papaya L.)
A-130.1
Limit test for rutin and quercetin in Ginkgo leaf dried extract (Ginkgo biloba L.)
A-129.1
Limit test for quercetin in Ginkgo leaf dried extract (Ginkgo biloba L.) – reverse phase method
A-128.1
Identification of Tangerine Peel (Citrus reticulata Blanco)
A-127.1
Identification of Rhodiola crenulata roots and rhizomes (Rhodiola crenulata (Hook. f. & Thomson) H. Ohba)
A-126.1
Identification of Lonicera macranthoides flower (Lonicera macranthoides (Thunb.)
A-125.1
Identification of Lonicera japonica flower (Lonicera japonica (Thunb.)
A-124.1
Identification of Banaba leaves (Lagerstroemia speciosa (L.) Pers.)
A-123.1
Identification of Garcinia indica fruits (Garcinia indica (Thouars) Choisy)
A-122.1
Identification of Garcinia gummi-gutta fruits (Garcinia gummi-gutta (L.) N. Robson)
A-121.1
Identification of Astragalus root (Astragalus membranaceus (Fisch.) Bunge and Astragalus membranaceus var. mongholicus (Bunge) P. K. Hsiao)
A-120.1
Strategy for rapid separation, detection and quantification of explosives by HPTLC 
A-119.1
Identification of Cola nitida seed (Cola nitida (Vent.) Schott & Endl.)
A-118.1
Screening method for identification of forensic drugs
A-117.1
Densitometric quantification of two steviol glycosides in Stevia leaf dry extract (Stevia rebaudiana (Bertoni) Bertoni)
A-116.1
Identification of Yerba mate (Ilex paraguariensis A.St.-Hil.)
A-115.1
Identification of Curcuminoids extract according to USP DSC 2018
A-114.1
Identification of wintergreen oil by HPTLC and discrimination of pure (synthetic) methyl salicylate
A-113.1
Densitometric quantification of oleuropein in Olive leaf dry extracts (Olea europaea L.)
A-112.1
Limit test for caffeine content (< 0.1% according to the USP 40 NF35)
A-111.1
Identification of Decaffeinated Green Tea Dry Extract according to USP DSC 2018
A-110.1
Identification of Olive leaf (Olea europaea L.)
A-109.1
HPTLC test for minimum content on 18β-glycyrrhizic acid (CAS 1405-86-3) in Licorice root (Glycyrrhiza glabra and Glycyrrhiza uralensis) based on the acceptance criteria of USP 39 NF 34
A-108.1
Identification and quantification of different cannabinoids in Cannabis sativa
A-107.1
Screening for phenethylamines in pre-workout supplements (boosters)
A-106.1
Quantification of nicotine in liquids for electronic cigarettes
A-105.1
Detection of steroids and SARMs
A-104.1
HPTLC fingerprint of Edelweiss plants and extracts used as ingredients in cosmeceuticals
A-103.1
Detection of UV filters in cosmetic products by HPTLC and confirmation by HPTLC-MS
A-102.1
Adulteration of St. Johns Wort Products: An HPTLC-MS Investigation
A-101.1
Determination of SARA in bitumen by HPTLC
A-100.1
Detection of sibutramine in slimming products by HPTLC and confirmation by HPTLC-MS
A-099.1
In-process control of ergoline psychedelics during chemical synthesis by HPTLC
A-098.1
Confirming the presence of cannabinoids in Cannabis sativa by HPTLC-MS
A-097.1
Determination of aflatoxins B1, G1, B2 and G2 in tomato extract by HPTLC
A-096.1
Rapid content uniformity test of 6 batches of Coenzyme Q10 in soft gel capsules by HPTLC
A-095.1
Determination of metronidazole in pharmaceutical dosage form by HPTLC
A-094.1
Determination of illegal dyes in spices by HPTLC
A-093.1
Determination of the flavonoid rutin in Ginkgo biloba dry extract by HPTLC
A-092.1
Determination of ginkgolides A, B, C and bilobalide in Ginkgo biloba dry extract by HPTLC
A-091.1
Determination of phospholipids by HPTLC
A-090.1
HPTLC method for determination of ceramides from human skin
A-089.1
HPTLC method for the determination of apolar lipids from human skin
A-088.1
Determination of melamine in milk by HPTLC
A-087.1
Determination of aucubin and catalpol in leaves of Ribwort Plantain (Plantago lanceolata)
A-086.1
Artemisinin in Artemisia annua leaf
A-085.1
Determination of Capsaicin in Capsicum tincture by HPTLC
A-084.1
Determination of tetrandrine in Han fangji (Stephania tetrandra)
A-083.1
Determination of sucralose and fructose in food and beverages by HPTLC
A-082.1
Impurities in bupropion hydrochloride
A-081.2
Oligosaccharides in molasses
A-041.3
Glycerol in tobacco
A-014.4
Acetylsalicylic acid, salicylic acid, and salicyl amide by HPTLC
A-012.4
Determination of aflatoxins B1, B2, G1, and G2 in foodstuffs
A-011.4
Quantitative determination of amino acids in potatoes
A-009.1
HPTLC screening method for antioxidant properties of substances in various matrices using DPPH
A-008.1
Biological activity of berberine containing drugs by HPTLC-Bioluminescence screening