Rapid Commun. Mass Spectrom. 24, 1305-1311 (2010). HPTLC of salvinorin A (1), salvinorin C (2), divinatorin B (3), and salvinorin B (4) in the leaves of Salvia divinorium on silica gel with methyl tert-butyl ether – hexane 3:1 as mobile phase. Detection by desorption electrospray ionization multi-stage tandem mass spectrometry (TLC/DESI-MS). The hRf were 49, 64, 85, and 95 for compounds (1) - (4), respectively. The use of a simple HPTLC protocol in combination with DESI-MS allowed for improved detection of different species of salvinorin in the leaf extracts.

CBS 103, 10-12 (2009). HPTLC of plant extracts and standards chlorogenic acid, hyperoside, rutin, quercetin and kaempferol (0.1 % in methanol) on silica gel in a twin-trough chamber with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:27. Detection by spraying with various detection reagents: 1) natural products reagent, evaluation under UV 366 nm, 2) anisaldehyde reagent, evaluation under white light, 3) diphenyl-2-picrylhydrazyl reagent (DPPH radical), evaluation under white light, 4) rhodamine B reagent, evaluation under UV 366 nm, 5) Dragendorff reagent, evaluation under white light.

J. Planar Chromatogr. 23, 255-259 (2010). Synthesis of a new ligand by anchoring anthracene to L-methionine. The ligand allows identification of amino acids on TLC plates based on distinguishable colors. A theoretical calculation (Hartree–Fock) was performed to investigate interaction of the ligand with the amino acids. TLC of 22 amino acids on silica gel with n-propanol - water 7:3. Detection by spraying with 0.01 % anthracenylmethyl L-methionine in acetone, followed by drying and spraying with 0.25 % ninhydrin in acetone. After drying and heating at 110 °C for 10 min the zones were evaluated visually. Detection limits were between 1 and 200 ng/zone.

J. Tech. & Sci. of Ningxia Agr. & Forest, 5, 38-39 (2010). For liquorice TLC on silica gel with ethyl acetate - glacial acetic acid - formic acid - water 15:1:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC. For Rhizoma zingiberis TLC on silica gel with cyclohexane - diethyl ether 1:1, detection by spraying with 10% vanillin in sulfuric acid and heating at 105 ºC, followed by evaluation in daylight.

J. Chromatogr. A 1218 (19), 2754-2774 (2011). HPTLC for lipid analysis is particularly useful for smaller, apolar compounds and offers some advantages over HPLC. Description of stationary phases, solvent systems and detection methods for the individual lipid classes (cholesterol and its derivates, glycerides, sphingo- and glycolipids, phospholipids). In comparison with common staining methods the combination of HPTLC and mass spectrometric detection methods is a very powerful method to investigate the identities of the HPTLC zones in detail.

Acta Chromatographica 20(3), 283-307 (2008). TLC of twenty flavonoids and their analogues on different stationary phases (non-polar and polar bonded stationary phases, silica gel, amino phase, diol phase) developed with a variety of binary mobile phases (aqueous and non-aqueous). Evaluation of similarities and differences among the chromatographic systems by principal component analysis and hierarchical clustering. Application of scoring indices to the separation power of a given system or a pair of systems allowed selection of the most suitable systems either to perform two-dimensional separations or to enhance the overall resolution by merging two stationary phases. On the basis of the investigation relatively efficient two-dimensional system on amino phase were developed. TLC with tetrahydrofuran – water 9:1 in the first dimension and acetonitrile – water 9:1, 4:1, 3:1, or 7:3 in the second dimension was found to be suitable for the separation of the compounds. Theoretically the compounds were best separated by combining diol and amino phases and using methanol – water 3:2 and acetonitrile – water 9:1, respectively.

Acta Chromatographica 22 (3), 433-443 (2010). HPTLC on silica gel with ethyl acetate – methanol - acetic acid 13:2:1. The hRf values were 46 and 78 for nebivolol hydrochloride and hydrochlorothiazide, respectively. Detection and quantification by densitometry at 280 and 270 nm for nebivolol hydrochloride and hydrochlorothiazide, respectively. The drugs were subjected to hydrolysis under acidic, basic, and neutral conditions, oxidation, heat, and photolysis as stress conditions. The drug showed degradation when subjected to oxidative stress and acidic conditions, which also affected the tablet sample substantially. However there was no interference of the drug peak by any of the degradation products. The method was therefore applied for stability testing of these drugs during stability studies.

Acta Chromatographica 22 (1), 91-97 (2010), DOI:10.1556/AChrom.22.2010.1.7. TLC on silica gel with chloroform – methanol – toluene 4:3:3. The hRf value was 77 and 29 for cefuroxime axetil and potassium clavulanate, respectively. Quantification by densitometry at 225 nm. The linearity was in the range of 0.5–2.5 and 2–10 µg/band, respectively. Application of the method for analysis of the drugs in a pharmaceutical formulation with a recovery of 100.1 % for cefuroxime axetil and 99.9 % for potassium clavulanate.