Anal. Bioanal. Chem. https://doi.org/10.1007/s00216-023-05101-y (2024). HPTLC of monogalactosyldiacylglycerol (1), sulfoquinovosyl diacylglycerol (2), and phosphatidylglycerol (3) in microalgae strains Nannochloropsis granulata, Phaeodactylum tricornutum, Porphyridium purpureum, and Tetraselmis tetrathele on silica gel with methyl acetate - isopropanol - chloroform - methanol - 0.25 % aqueous KCl solution (acidified with glacial acetic acid) 500:500:500:200:87, n-hexane - acetone - isopropanol 16:4:1 and finally with n-hexane - diethyl ether - glacial acetic acid 70:30:1. Detection by dipping into a modified copper sulfate reagent (20 g of CuSO4 × 7 H2O in 200 mL of methanol and acidified with 8 mL of 96 % sulfuric acid and 8 mL of 85 % orthophosphoric acid) for 6 s, followed by heating at 140 °C for 30 min. Quantitative determination by absorbance measurement at 720 nm. Linearity was in the range of 100-2100 ng/zone. Intermediate precisions were below 4 % (n=3). The LOD and LOQ were in the range of 18-29 ng/zone and 63-90 ng/zone, respectively. Recovery was in the range of 93.1-108.1 %.

Chinese J. Ethnomed. & Ethnopharm. 32 (22), 42-46 (2023). Sophora alopecuroides is a medicinal plant growing in Northern China, containing alkaloids, flavonoids, volatile oils and amino acids. It has antipyretic detoxification, antibacterial and anti-inflammatory, as well as analgesic effects, and is the main effective ingredient in some TCM mixtures widely used in clinical practice. This research team developed a medicinal gel, Kudouzi Ningjiaoji gel, which has good clinical results in the treatment of acne, pustules and other diseases. For quality control, TLC of the extracts of Kudouzi Ningjiaoji gel on silica gel with dichloromethane – methanol – ammonia water 50:6:1. Detection in daylight after spraying with a solution of bismuth potassium basic nitrate 0.85 g, glacial acetic acid 10 mL, water 40 mL and potassium iodide 2 g. Identification by comparing of sample fingerprints with those standards sophoridine and matrine.

J. Ethnopharmacol. 320, 117404 (2024). HPTLC of lupeol and stigmasterol in the stem bark of Ficus benghalensis, Ficus glomerata, Ficus religiosa, Ficus infectoria, and Albizia lebbeck on silica gel with toluene - ethyl acetate - methanol - formic acid 38:5:5:20:1. Detection by spraying with anisaldehyde sulfuric acid. Qualitative identification under UV light at 525 nm.

J. Ethnopharmacol. 318, 117004 (2024). HPTLC of gallic acid in Pyracantha crenulata on silica gel with toluene - ethyl acetate - formic acid 8:10:3. Qualitative identification under UV light at 254 nm. The hRF value of gallic acid was 74. 

J. Ethnopharmacol. 321, 117566 (2024). HPTLC of friedelin in Euphorbia grantii on silica gel with hexane – ethyl acetate 19:1. Detection by spraying with para anisaldehyde, followed by heating at 105 °C. Quantitative determination by absorbance measurement at 580 nm. LOD and LOQ were 0.5 and 1.8 μg/zone, respectively. 

J. Chinese Pharm. 34 (1), 52-56 (2023). Inonotus obliquus is a new source of fungal medicine, containing polysaccharides, polyphenols, triterpenoids, sterols and folic acid derivatives, with various pharmacological effects such as anti-tumor, hypoglycemic, antioxidant, and antiviral. For identification, TLC of its extracts on silica gel, A) for thrombotic acid, with chloroform – toluene – ethyl acetate – methanol 10:4:1:1; and B) for inotodiol, with dichloromethane – toluene – ethyl acetate – methanol 100:40:3:3. Detection for both by spraying with 5 % phosphomolybdate in ethanol and heating at 105°C until the zones are visible in daylight. Identification by fingerprint comparison with the standard drug Inonotus obliquus and the standards thrombotic acid and inotodiol. Chemical pattern recognition analysis was applied on resulting TLC and HPLC data (such as, cluster analysis, principal component analysis, orthozonal least squares-discriminant analysis) to evaluate the quality of the medicine.

Chinese J. Veter. Drug 57 (6), 52-57 (2023). Wupi Koufuye is a multi-ingredient TCM veterinary drug clinically used for the prophylaxis and treatment of gout in animals. For quality control, TLC on silica gel A) for White Mulberry root bark, developed twice with cyclohexane – ethyl acetate – methanol – concentrated ammonia solution 75:75:15:2, detection at UV 366 nm; B) for Pericarpium citri reticulatae, developed with trichloromethane - methanol – concentrated ammonia solution 100:30:3, detection by spraying with 0.5 % ninhydrin in ethanol and heating at 105 °C until the zones are visible in daylight; C) for Zingiber officinale Rosc., with n-hexane, detection by spraying with 0.5 % vanilin in sulfuric acid – ethanol 4:1 followed by heating at 105°C until the zones are visible in daylight.

J. China Pharm. 32 (6), 71-74 (2023). Shegan Kangbingdu Koufuye is a multi-ingredient TCM oral liquid with antiviral effects. For quality control, TLC on silica gel A) for Blackberrylily rhizome, with chloroform – methanol – glacial acetic acid 200:30:2, detection by spraying with 2 % FeCl3 in ethanol, evaluation in fluorescence; B) Radix Bupleuri,  with chloroform – methanol – water 13:7:2, detection by spraying with 2 % 4-dimethylaminobenzaldehyde in 40 % sulfuric acid solution and heating at 60 °C until the zones are visible at UV 366 nm; C) for Folium Isatidis and Radix Isatidis, with cyclohexane - chloroform – propanone 5:4:2, detection in fluorescence light; D) for Capillary Wormwood Herb, Honeysuckle and Dandelion, with toluene – ethyl formate – formic acid 6:4:1, detection in fluorescence light.