Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
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Food Chem. 88, 2902-2918 (2023). HPTLC of high free fatty acid, monoacylglycerols, diacylglycerols and triacylglycerol on silica gel with hexane – diethyl ether - acetic acid 50:50:1.
Biochem. Biophys. Res. Commun. 667, 146-152 (2023). HPTLC of intracellular lipids in sebocytes treated with calcium for 24 h and incubated with medium containing 2 µCi of [1-14C] on silica gel with hexane - ethyl acetate 6:1. Detection by autoradiography. The method allowed the identification of squalene, trygliceride and cholesterol.
Food Res. Int. 165, 112472 (2023). HPTLC of galactolipids (monogalactosyldiacylglycerol and digalactosyldiacylglycerol) in chloroplast-rich pellet from spinach leaves on silica gel with chloroform - methanol - water 19:4:5. Detection by dipping into a thymol solution (1 g of thymol in 190 mL of ethanol following with a slow addition of 10 ml of sulphuric acid), followed by heating at 110 °C for 10 min. Image visualization under white light and under UV light at 254 and 366 nm.
Marine Drugs 20(4), 270 (2022). Samples were ether glycerols (EG) purified: (A) from Chimaera monstrosa liver oil (Chimaeridae); (B) from mixed liver oil of sharks Centrophorus squamosus (Centrophoridae) and Somniosus microcephalus (Somniosidae); (C) from Macaca fascicularis hearts (Cercopithecidae); (D) from tumors obtained by grafting in mice the human melanoma cell line MDA-MB-435s, and (E) from periprostatic adipose tissue of men with prostate cancer. Reduction of (phospho)ester glycerolipids into EG and fatty alcohols was part of the purification process. Octadecyl-glycerol and octadecenyl-glycerol were used as standards of alkyl- and alkenyl-glycerols, respectively. HPTLC on silica gel previously developed with chloroform – methanol 1:1, air-dried and activated for 30 min at 110° C. Application under nitrogen stream (6 bar). Development with petroleum ether – diethyl ether – acetic acid 60:140:1. After 2 h drying at room temperature under ventilation hood, visualization by 50 s immersing into sulfuric acid (7 % in ethanol), followed by 2 h drying under air-stream, and 14 min heating at 140° C. Plates were documented under white light illumination and densitometry was performed by computered scanning of the pictures. Alkyl-glycerols (mean hRF 34, LOQ 1235 ng/band) and alkenyl-glycerols (mean hRF 44, LOQ 2352 ng/band), present in all samples (except alkenyl-glycerols in shark oil), were quantified after method validation for specificity, sensitivity, accuracy, precision and repeatability. Linearity range was 1000 ng – 7000 ng for both EG types. To confirm the band identification, samples and standards were also submitted to acidic hydrolysis before HPTLC application. In this case, the bands of alkenyl glycerols did not appear, because chlorhydric acid reacted with the vinyl ether bonds to form glycerol and aldehydes.
J Chromatogr A 1638, 461895 (2021). Samples were sphingolipid-rich fractions of unproteinated blood plasma from healthy humans or from Fabry’s disease patients, as well as standards of sphingomyelin (SM) and of globotriaosylceramides (Gb3 = ceramide trihexosides), and related compounds (lyso-ceramide trihexosides, lactosyl ceramide, glucosyl ceramide). HPTLC on silica gel (Lichrosphere with spherical particles) by automated multiple development with a 9-step gradient, starting with pure methanol and ending with dichloromethane – methanol 9:1. Visualization and densitometry under UV 190 nm. Derivatization for Gb3 and derivatives (but not for SM) by immersion into orcinol solution (0.2 %, with sulfuric acid 10 %), followed by 15 min heating at 100 °C and by densitometry under visible light 550 nm. Bands of interest were directly eluted with methanol from underivatized plates into an ion-trap MS, through the oval head of a TLC-MS interface (with stainless steel frit to remove silica gel particles). Two different ionization processes were used: (A) electrospray ionization (ESI, capillary voltage 4 kV, endplate offset voltage -0.5 kV, nebulizer pressure 40 psi, drying gas 9 mL/min at 350 °C); (B) atmospheric pressure chemical ionization (APCI, capillary voltage 2–3 kV, current intensity 4.5 µA, nebulizer pressure 45 psi, drying gas 5 mL/min at 350 °C; vaporization at 450 °C). Full MS spectra were recorded up to m/z 1500 in positive ion mode. The relative ion intensities were used to quantify the detected species. Previous to this study, the precision of the elution head positioning was tested on Gb3 standard zones, comparing 3 positions for analyte elution: from the centre and from each higher or lower side of the band. The same main m/z peaks were observed in the 3 positions, but in different proportions. This was explained by the presence of coeluting Gb3 subclasses (the ceramide moiety CM being either saturated, mono-unsaturated fatty acyl with a slightly higher migration distance, or polar hydroxyl fatty acyl with the opposite effect on migration) and of coeluting Gb3 isoforms (the hexoside moiety consisting of glucose and/or galactose units). This resulted in the broadening and partial splitting of the standard band. In the plasma samples, 19 molecular species of Gb3 were identified (depending on the CM, the sugar isoforms being undistinguishable by MS): 5 with a saturated CM, 7 with two additional double bonds on the CM, 7 with a methylated CM. In case of Fabry’s disease, most Gb3 species with saturated CM were highly increased, whereas other species were decreased.
Microbiol. Res. 267, 127260 (2023). HPTLC of lipids in a Glutamicum mutant ΔSYA, unable to synthesize trehalose constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis, on silica gel with chloroform - methanol - water - ammonia 65:25:1:3. Detection by spraying with 10 % sulfuric acid in methanol, followed by drying at 160 °C for 5 min. Further analysis by liquid chromatography mass spectrometry.
Microbiol. Res. 268, 127295 (2023). HPTLC of cardiolipin phospholipids in the deletion mutants of two cardiolipin synthetase genes, clsA (UM270 ΔclsA) and clsB (UM270 ΔclsB), in the rhizobacterium Pseudomonas fluorescens, on silica gel with chloroform - methanol - water 14:6:1 for the first dimension and chloroform - methanol - glacial acetic acid 13:5:2 for the second dimension. The lipid composition of UM270 wt, UM270 ΔclsA and UM270 ΔclsB mutant strains was determined by labeling with [1-14C] acetate. Detection by iodine staining and radioactive membrane lipids were visualized by exposure to autoradiography film or a Phosphor Imager screen. Individual lipids were quantified using an image software.
Trends Anal. Chem. 157, 116808 (2022). Review of recent advances in methods for the analysis of cardiolipin in different biological samples. The paper described analytical methods such as TLC and HPTLC for the study of cardiolipin abnormalities in neurological disorders, cancer, cardiovascular and metabolic diseases. Advantages and disadvantages of different techniques available to detect and quantify cardiolipin were also discussed.