Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      129 019
      Rapid analytical approach for bioprofiling compounds with radical scavenging and antimicrobial activities from seaweeds
      P. RISTIVOJEVIC, V. JOVANOVIC, D. OPSENICA, J. PARK, Judith ROLLINGER, Tanja VELICKOVIC* (*University of Belgrade–Faculty of Chemistry, Studentski trg 12-16, 11158 Belgrade, Serbia, anja.velickovic@ghent.ac.kr)

      Food Chem. 334, 127562 (2021). HPTLC of five seaweed cultivars, namely three Saccharina japonica and two Undaria pinnatifida on silica gel with n-hexane - ethyl acetate - formic acid 30:50:1. Detection by spraying with anisaldehyde sulfuric acid reagent (1.5 mL of anisaldehyde was mixed with 210 mL of ethanol, 25 mL acetic acid and 13 mL conc. sulfuric acid), followed by heating at 120 °C for 3 min. Qualitative identification under UV light at 366 nm. HPTLC-bioautography antimicrobial assays by dipping into B. subtilis cell suspension, followed by incubation at 37 °C for 30 min and E. coli suspension, followed by incubation at 37 °C for 1 h. Visualization by dipping into a solution of MTT dye with triton X-100 (1 mg/mL). Stearidonic, eicosapentaenoic, and arachidonic acids were identified by HPLC-MS.

       

      Classification: 11c
      129 002
      Imaging high-performance thin-layer chromatography as powerful tool to visualize metabolite profiles of eight Bacillus candidates upon cultivation and growth behavior
      S. KRUSE, F. PIERRE, Gertrud E. MORLOCK* (*Institute of Nutritional Science, and Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany; gertrud.morlock@uni-giessen.de)

      J Chromatogr A, 1640, 461929 (2021). Study of the impact of different strains, culture media and parameters (temperature, time, rotational speed, and glucide and amino-acid supply) on the metabolite profile of bacteria. Samples were cultivation broths of Bacillus subtilis, B. licheniformis, B. pumilus and B. amyloliquefaciens, as well as their respective supernatant liquid-liquid extracts (apolar solvents only or QuEChERS method with acetonitrile and MgSO4 – NaCl mixture 4:1). HPTLC on silica gel (normal phase and RP-18), either as bands (for small volumes of extracts) or as areas for supernatants and bigger volumes of extracts. Extract areas were focused with a three-step procedure (up to 20mm with acetone, and twice with methanol); unextracted supernatants were focused twice with methanol and once with tetrahydrofuran, but the application zone of the plate had to be cut before development, due to the high matrix load. Development with ethyl acetate – methanol – water at different ratios after activation of the plate surface with magnesium chloride (33% relative humidity), evaluation in white light and UV. Detection of antibacterial compounds with Aliivibrio fischeri bioassay. Derivatization with primuline (for lipophilic substances) and diphenylamine aniline sulfuric acid reagent (for saccharides). This method allowed a fast comparison: A) of the patterns of the different strains (presence /absence and intensity of detected or antibacterial bands); B) of cultivation parameters: the number of metabolites increased with time, rotational speed (oxygen level), and at 37°C (vs. 30°C), whereas a minimal medium allowed the detection of more metabolites, due to the lower matrix load; C) of the impact of the extraction parameters: choice of the solvents (QuEChERS method had no advantage here), solvent – supernatant ratio (1:3 showed richer patterns than 1:1); D) of the HPTLC parameters used (better separation and resolution with normal phase vs. RP18 layers).

      Classification: 3a, 10a, 11c, 27
      128 048
      Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice
      K. IRUNGBAM, M. RODERFELD, H. GLIMM, F. HEMPEL, F. SCHNEIDER, L. HEHR, D. GLEBE, Y. CHURIN, G. MORLOCK, I. YÜCE, Elke ROEB* (*Department of Gastroenterology, Justus Liebig University Giessen, Giessen, Germany; elke.roeb@innere.med.uni-giessen.de)

      Nature - Lab. Invest. 100, 1411–1424 (2020). Samples were chloroform – methanol 1:1 solutions of lipid standards and of liver tissue extracts from wild-type mice (1), from transgenic murine models of hepatic steatosis (2) (mice expressing HBs, hepatitis B virus surface protein), or of cholestasis (3) (mice totally knock-out for the gene of phospholipid translocator ABCB4, ATP-binding cassette subfamily B member 4), or of both (4) (hybrids of mice (2) and (3)). HPTLC on silica gel (preheated at 110°C for 15 min) with n-hexane – diethyl ether – acetic acid 20:5:1. (A) For qualitative analysis, visualization under white light after immersion into anisaldehyde 0.5 % (in sulfuric acid – acetic acid – methanol, 1:2:17), followed by heating at 110°C for 9 min. (B) Identification of lipids was confirmed by elution of the zones of interest with methanol from the HPTLC layer through a TLC-MS interface and a filter frit directly to a quadrupole-orbitrap MS (atmospheric pressure chemical ionization, full HR-MS scan in m/z range 100–1000). (C) For quantitative analysis, visualization at UV 366 nm after derivatization by immersion into primuline reagent (primuline 0.5 g/L in acetone – water 4:1); fluorescence was measured at UV 366 nm (mercury lamp, optical filter for wavelengths above 400 nm, scanning slit 6.0 mm × 0.2 mm, speed 20 mm/s). (A) and (B) allowed the separation and detection of cholesterol, cholesteryl oleate, methyl oleate, free fatty acids (FFA, expressed as oleic acid equivalents) and triacylglycerols (TAG, as triolein equivalents) in liver extracts. (C) showed that TAG was decreased and FFA increased in (3) and (4), compared to (1) and (2). Cholesterol and cholesteryl oleate had no significant changes between groups.

      Classification: 4e, 11a, 11c, 13c
      128 004
      Validation and application of a protocol for the extraction and quantitative analysis of sphingomyelin in erythrocyte membranes of patients with non‑alcoholic fatty liver disease
      C. PAPADOPOULOS, K. MIMIDIS, I. TENTES, T. TENTE, K. ANAGNOSTOPOULOS* (*Laboratory of Biochemistry, Department of Medicine, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, Greece, kanagnos@med.duth.gr; kanagno@gmail.com)

      J. Planar Chromatogr. 34, 411-418 (2021). HPTLC of sphingomyelin in erythrocyte membranes of patients on silica gel with chloroform - methanol - acetic acid - water 60:50:1:4. Detection by exposure to iodine vapor for 30 min. Quantitative determination by densitometric measurement of the intensity of individual zones and the red, green and blue values of the component colors. The hRF value for sphingomyelin was 86. Linearity was between 0.25 and 10 μg/zone. LOD and LOQ were 140 and 410 ng/zone, respectively. Intermediate precisions were below 2 % (n=3). Recovery was between 85 and 97 %.

      Classification: 11c
      128 030
      Detection of edible insect derived phospholipids with polyunsaturated fatty acids by thin-layer chromatography, gas chromatography, and enzymatic methods
      M. OCHIAI*, Y. KOMIYA (*Laboratory of Animal and Human Nutritional Physiology, School of Veterinary Medicine, Kitasato University, Higashi 23-35-1 Towada, Aomori, 034-8628, Japan, mochiai@vmas.kitasato-u.ac.jp)

      J. Food Compos. Anal. 99, 103869 (2021). HPTLC of phosphatidylcholine (1), phosphatidylethanolamine (2), phosphatidylserine (3), lysophosphatidylcholine (4), and cardiolipin (5) in edible insects (crickets, migratory locusts, and silkworms) on silica gel with chloroform - methanol - 28 % ammonia 13:7:1. Detection by exposure to iodine. The hRF values for (1) to (5) were 24, 51, 13, 15 and 70, respectively. 

      Classification: 11c
      127 018
      Effects of oil pollution and persistent organic pollutants (POPs) on glycerophospholipids in liver and brain of male Atlantic cod (Gadus morhua)
      Mari BRATBERG, P. OLSVIK, R. EDVARDSEN, H. BREKKEN, R. VADLA, S. MEIER* (*Institute of Marine Research, P.O. Box 1870, N-5817 Nordnes, Bergen, Norway, sonnich.meier@imr.no)

      Chemosphere. 90, 2157-2171 (2013). HPTLC of glycerophospholipids in liver and brain of male Atlantic cod (Gadus morhua) on silica gel (washed with methyl acetate - isopropanol - chloroform - methanol - 0.25 % KCl 25:25:25:10:9 and activated at 120 ºC for 30 min) with hexane - diethylether - acetic acid 40:10:1. Detection by spraying with 0.1 % dichlorofluorescin in 98 % methanol and 0.001 % 3,5-di-tert-4butylhydroxytoluene (BHT). Lipid classes were futher analyzed by gas chromatography with a flame ionization detector. 

      Classification: 11c
      127 025
      In vitro assessment of pediococci- and lactobacilli-induced cholesterol-lowering effect using digitally enhanced high-performance thin-layer chromatography and confocal microscopy
      R. SYAKILA, S. LIM, S. KUSTRIN, F. LIM, K. RAMASAMY* (*Faculty of Pharmacy, University Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia, kalav922@gmail.com)

      Anal. Bioanal. Chem. 411, 1181-1192 (2019). HPTLC of cholesterol in fresh egg yolk on silica gel with n-hexane - ethyl acetate - acetic acid 15:9:1. Detection by dipping into p-anisaldehyde sulfuric acid reagent solution for 1 s (1 mL p-anisaldehyde with a refrigerated solution of glacial acetic acid/concentrated sulfuric acid in methanol in a ratio of 1:2:100). Quantitative determination by absorbance measurement at 525 nm. Evaluation under daylight and UV light. The method allowed to study cholesterol-lowering properties of 12 lactic acid bacteria (LAB) in the absence or presence of 0.3 % bile salts. The hRF value for cholesterol was 71. Linearity was between 1 and 10 μg. Intermediate precision was below 7 % (n=3). The LOD and LOQ for cholesterol were 0.2 and 0.8 μg, respectively. Recovery was between 96.6 and 100.1 %. 

      Classification: 11c
      127 034
      Differences in the lipid patterns during maturation of 3T3-L1 adipocytes investigated by thin-layer chromatography, gas chromatography, and mass spectrometric approaches
      Yulia POPKOVA*, D. DANNENBERGER, J. SCHILLER, K. ENGEL (*Institute for Medical Physics and Biophysics, Medical Faculty, Leipzig University, Härtelstr. 16-18, 04107 Leipzig, Germany, yulia.popkova@medizin.uni-leipzig.de)

      Anal. Bioanal. Chem. 412, 2237-2249 (2020). HPTLC of sphingomyelin (1), phosphatidylcholine (2), and triacylglycerol (3) fractions from preadipocytes and adipocytes on silica gel with chloroform - ethanol - water - triethylamine 30:35:7:35 for (1) and (2) and hexane - diethyl ether - acetic acid 80:20:1 for (3). Detection by dipping into primuline (dissolved in acetone - water 4:1) and under UV light at 366 nm. Lipid fractions were further analyzed by electrospray ionization-ion trap mass spectrometry.

      Classification: 11c