Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      128 048
      Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice
      K. IRUNGBAM, M. RODERFELD, H. GLIMM, F. HEMPEL, F. SCHNEIDER, L. HEHR, D. GLEBE, Y. CHURIN, G. MORLOCK, I. YÜCE, Elke ROEB* (*Department of Gastroenterology, Justus Liebig University Giessen, Giessen, Germany; elke.roeb@innere.med.uni-giessen.de)

      Nature - Lab. Invest. 100, 1411–1424 (2020). Samples were chloroform – methanol 1:1 solutions of lipid standards and of liver tissue extracts from wild-type mice (1), from transgenic murine models of hepatic steatosis (2) (mice expressing HBs, hepatitis B virus surface protein), or of cholestasis (3) (mice totally knock-out for the gene of phospholipid translocator ABCB4, ATP-binding cassette subfamily B member 4), or of both (4) (hybrids of mice (2) and (3)). HPTLC on silica gel (preheated at 110°C for 15 min) with n-hexane – diethyl ether – acetic acid 20:5:1. (A) For qualitative analysis, visualization under white light after immersion into anisaldehyde 0.5 % (in sulfuric acid – acetic acid – methanol, 1:2:17), followed by heating at 110°C for 9 min. (B) Identification of lipids was confirmed by elution of the zones of interest with methanol from the HPTLC layer through a TLC-MS interface and a filter frit directly to a quadrupole-orbitrap MS (atmospheric pressure chemical ionization, full HR-MS scan in m/z range 100–1000). (C) For quantitative analysis, visualization at UV 366 nm after derivatization by immersion into primuline reagent (primuline 0.5 g/L in acetone – water 4:1); fluorescence was measured at UV 366 nm (mercury lamp, optical filter for wavelengths above 400 nm, scanning slit 6.0 mm × 0.2 mm, speed 20 mm/s). (A) and (B) allowed the separation and detection of cholesterol, cholesteryl oleate, methyl oleate, free fatty acids (FFA, expressed as oleic acid equivalents) and triacylglycerols (TAG, as triolein equivalents) in liver extracts. (C) showed that TAG was decreased and FFA increased in (3) and (4), compared to (1) and (2). Cholesterol and cholesteryl oleate had no significant changes between groups.

      Classification: 4e, 11a, 11c, 13c
      128 004
      Validation and application of a protocol for the extraction and quantitative analysis of sphingomyelin in erythrocyte membranes of patients with non‑alcoholic fatty liver disease
      C. PAPADOPOULOS, K. MIMIDIS, I. TENTES, T. TENTE, K. ANAGNOSTOPOULOS* (*Laboratory of Biochemistry, Department of Medicine, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, Greece, kanagnos@med.duth.gr; kanagno@gmail.com)

      J. Planar Chromatogr. 34, 411-418 (2021). HPTLC of sphingomyelin in erythrocyte membranes of patients on silica gel with chloroform - methanol - acetic acid - water 60:50:1:4. Detection by exposure to iodine vapor for 30 min. Quantitative determination by densitometric measurement of the intensity of individual zones and the red, green and blue values of the component colors. The hRF value for sphingomyelin was 86. Linearity was between 0.25 and 10 μg/zone. LOD and LOQ were 140 and 410 ng/zone, respectively. Intermediate precisions were below 2 % (n=3). Recovery was between 85 and 97 %.

      Classification: 11c
      128 030
      Detection of edible insect derived phospholipids with polyunsaturated fatty acids by thin-layer chromatography, gas chromatography, and enzymatic methods
      M. OCHIAI*, Y. KOMIYA (*Laboratory of Animal and Human Nutritional Physiology, School of Veterinary Medicine, Kitasato University, Higashi 23-35-1 Towada, Aomori, 034-8628, Japan, mochiai@vmas.kitasato-u.ac.jp)

      J. Food Compos. Anal. 99, 103869 (2021). HPTLC of phosphatidylcholine (1), phosphatidylethanolamine (2), phosphatidylserine (3), lysophosphatidylcholine (4), and cardiolipin (5) in edible insects (crickets, migratory locusts, and silkworms) on silica gel with chloroform - methanol - 28 % ammonia 13:7:1. Detection by exposure to iodine. The hRF values for (1) to (5) were 24, 51, 13, 15 and 70, respectively. 

      Classification: 11c
      127 018
      Effects of oil pollution and persistent organic pollutants (POPs) on glycerophospholipids in liver and brain of male Atlantic cod (Gadus morhua)
      Mari BRATBERG, P. OLSVIK, R. EDVARDSEN, H. BREKKEN, R. VADLA, S. MEIER* (*Institute of Marine Research, P.O. Box 1870, N-5817 Nordnes, Bergen, Norway, sonnich.meier@imr.no)

      Chemosphere. 90, 2157-2171 (2013). HPTLC of glycerophospholipids in liver and brain of male Atlantic cod (Gadus morhua) on silica gel (washed with methyl acetate - isopropanol - chloroform - methanol - 0.25 % KCl 25:25:25:10:9 and activated at 120 ºC for 30 min) with hexane - diethylether - acetic acid 40:10:1. Detection by spraying with 0.1 % dichlorofluorescin in 98 % methanol and 0.001 % 3,5-di-tert-4butylhydroxytoluene (BHT). Lipid classes were futher analyzed by gas chromatography with a flame ionization detector. 

      Classification: 11c
      127 025
      In vitro assessment of pediococci- and lactobacilli-induced cholesterol-lowering effect using digitally enhanced high-performance thin-layer chromatography and confocal microscopy
      R. SYAKILA, S. LIM, S. KUSTRIN, F. LIM, K. RAMASAMY* (*Faculty of Pharmacy, University Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia, kalav922@gmail.com)

      Anal. Bioanal. Chem. 411, 1181-1192 (2019). HPTLC of cholesterol in fresh egg yolk on silica gel with n-hexane - ethyl acetate - acetic acid 15:9:1. Detection by dipping into p-anisaldehyde sulfuric acid reagent solution for 1 s (1 mL p-anisaldehyde with a refrigerated solution of glacial acetic acid/concentrated sulfuric acid in methanol in a ratio of 1:2:100). Quantitative determination by absorbance measurement at 525 nm. Evaluation under daylight and UV light. The method allowed to study cholesterol-lowering properties of 12 lactic acid bacteria (LAB) in the absence or presence of 0.3 % bile salts. The hRF value for cholesterol was 71. Linearity was between 1 and 10 μg. Intermediate precision was below 7 % (n=3). The LOD and LOQ for cholesterol were 0.2 and 0.8 μg, respectively. Recovery was between 96.6 and 100.1 %. 

      Classification: 11c
      127 034
      Differences in the lipid patterns during maturation of 3T3-L1 adipocytes investigated by thin-layer chromatography, gas chromatography, and mass spectrometric approaches
      Yulia POPKOVA*, D. DANNENBERGER, J. SCHILLER, K. ENGEL (*Institute for Medical Physics and Biophysics, Medical Faculty, Leipzig University, Härtelstr. 16-18, 04107 Leipzig, Germany, yulia.popkova@medizin.uni-leipzig.de)

      Anal. Bioanal. Chem. 412, 2237-2249 (2020). HPTLC of sphingomyelin (1), phosphatidylcholine (2), and triacylglycerol (3) fractions from preadipocytes and adipocytes on silica gel with chloroform - ethanol - water - triethylamine 30:35:7:35 for (1) and (2) and hexane - diethyl ether - acetic acid 80:20:1 for (3). Detection by dipping into primuline (dissolved in acetone - water 4:1) and under UV light at 366 nm. Lipid fractions were further analyzed by electrospray ionization-ion trap mass spectrometry.

      Classification: 11c
      127 080
      Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years
      V. CEBOLLA*, C. JARNE, J. VELA, R. GARRIGA, L. MEMBRADO, J. GALBAN (*Instituto de Carboquımica, ICB-CSIC, C/Miguel Luesma, 4, 50018 Zaragoza, Spain, vcebolla@icb.csic.es)

      J. Liq. Chromatogr. Relat. Technol. 44, 148-170 (2021). Comprehensive review of the contribution of HPTLC to the analysis of lipids in the last ten years. Advances in detection, identification and determination of main classes of lipids and their subclasses separated by HPTLC in different matrices were discussed. The review described techniques for scanning densitometry of lipids through direct detection and on-plate staining using inorganic and organic agents, immunostaining of glycosphingolipids and labeled lipids. Techniques coupling HPTLC with mass spectrometry were also discussed, including extraction-based interfaces, ionization of lipids, on-plate matrix assisted laser desorption and ionization, and its application in the analysis of sphingolipids, neutral lipids and fatty acids and phospholipids.

      Keywords: review
      Classification: 1b, 11c
      127 007
      Carotenoid composition and antioxidant potential of Eucheuma denticulatum, Sargassum polycystum and Caulerpa lentillifera.
      V. BALASUBRAMANIAM*, L. JUNE CHELYN, S. VIMALA, M.N. MOHD FAIRULNIZAL, I.A. BROWNLEE, I. AMINE (*Nutrition, Metabolism & Cardiovascular Research Centre, Institute for Medical Research, Ministry of Health Malaysia, Shah Alam, Selangor, Malaysia; vimala.rmt@moh.gov.my)

      Heliyon 6(8), e04654 (2020). HPTLC of ethanolic extracts of three algae (100µg/band) on silica gel, along with carotenoid standards (10µg/band), developed with toluene – acetone 7:3. Detection under white light. Carotenoids appeared orange or yellow, chlorophylls green, pheophytins dark khaki. Carotenoid patterns of the algae were very different depending on the family: red alga Eucheuma denticulatum (Solieriaceae) contained mainly zeaxanthin and lutein (hRF 44) and β-carotene (hRF 88), but also β-cryptoxanthin (hRF 69-71) and fucoxanthin (hRF 39); brown alga Sargassum polycystum (Sargassaceae) contained mainly fucoxanthin, and also cryptoxanthin; green alga Caulerpa lentillifera (Caulerpaceae) contained mainly zeaxanthin, but also astaxanthin (hRF 61) and canthaxanthin (hRF 77) in smaller amounts. Separately, HPLC-MS was used to confirm and quantify these compounds, which was necessary for carotenoids with similar hRF values: zeaxanthin and lutein (hRF 44), and β-carotene and lycopene (hRF 88).

      Classification: 11c, 15a, 23a, 32e