Planta Med. 83(09), 797-804 (2017). For the analysis of their sugar moieties, five new triterpene saponins (ilexpusons B, D, E, F, G), isolated from a methanolic extract of Ilex pubescens roots, were hydrolyzed at 90 °C in water containing HCl and dioxane. TLC of the dried aqueous phases on silica gel with chloroform – methanol – water 8:5:1. Detection by spraying with aniline – diphenylamine reagent (2 mL aniline, 2 g diphenylamine, 10 mL phosphoric acid 85%, in 100 mL acetone) and heating for 2-5 min at 85 °C. By comparison with standards, the sugar moiety was identified (before confirmation by GC) as glucose (hRF 49) in ilexpuson B, as xylose (hRF 81) in ilexpusons D and E, and as glucuronic acid (hRF 44) in ilexpusons F and G.

J. Planar Chromatogr. 31, 409-411 (2018). HPTLC of selected phospholipids (phosphatidic acid, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine) on silica gel with chloroform – ethanol – water– triethylamine 5:5:1:5. Selected experiments were performed with HPTLC plates impregnated with zinc chloride solution. Detection by spraying with a solution of primuline. The F254-fluorescence indicator affects the migration properties of the selected compounds_x000D_ in a mixture.

using high-performance thin-layer chromatography. J. Planar Chromatogr. 31, 309-317 (2018). HPTLC of gardenin-E (1), gardenin-D (2), xanthomicrol (3), 5-desmethynobiletin (4), gardenin-A (5), and gardenin-B (6) in the sum resin of Gardenia lucida on silica gel with n-hexane – diethyl ether – 1,4-dioxane 4:6:1. Detection by dipping into natural product reagent (10 mL of 1 % methanolic diphenyl boryloxyethylamine), followed by dipping into PEG reagent (8.0 mL of 5 % ethanolic polyethylene glycol 4000). Quantitative determination by absorbance measurement at 335 nm. Linearity was between 0.33 and 1.67 μg/zone. LOD and LOQ were 90 and 300 ng for (1), 90 and 290 ng for (2), 90 and 320 ng for (3), 100 and 320 ng for (4), 60 and 210 ng for (5) and 80 and 280 ng for (6), respectively. The intermediate precision was <2 %. Recovery ranged from 96.0 to 99.3 %.

J. Planar Chromatogr. 31, 445-450 (2018). HPTLC of benzyl isothiocyanate in the root of Salvadora persica with n-hexane. Quantitative determination by absorbance measurement at 254 nm. The hRF value for benzyl isothiocyanate was 39. Linearity was between 3–15 µg/zone. LOD and LOQ were 1 and 3 μg/zone. The intermediate precision was <2 % (n=3). Recovery was between 95.2 and 103.2 %.

J. Planar Chromatogr. 31, 461-468 (2018). HPTLC of dabigatran etexilate mesylate on silica gel with methanol ‒ ethyl acetate ‒ benzene 3:4:4. Quantitative determination by absorbance measurement at 225 nm. The hRF value for dabigatran etexilate mesylate was 82. Linearity was between 0.3 and 3 μg/zone. LOD and LOQ were 0.03 and 0.09 μg/zone. The intermediate precision was <2 % (n=3). Average recovery was 98.9 %.

Lipids 17, 477-482 (1982). Preparative TLC of microbially produced metabolites of lithocholic acid and lithocholic acid-3 alpha-sulfate on silica with dichloromethane-methanol 95:5. Detection by spraying with anisaldehyde, followed by heating. The following metabolites were isolated: isolithocholic acid, 5 beta-cholanic acid, 3-keto-lithocholic acid, 5 alpha -cholestone and delta 3-cholenic acid.

(Purity test of sorbose and sorbitol with thin-layer chromatography on Silufol ready made plates.) Pharmazie 39, 689-690 (1984). Sorbitol and sorbose are separated from concomitant substances such as glucose and/or fructose, disaccharides and trisaccharides. The silica layer pretreated with 2O mM solution of sodium pyrosulfate, then developed with 1-propanol - ethyl acetate - water 27:3.5:1 and visualized after drying at 100 °C by spraying with lead tetraacetate.

Chromatographia 18, 546-549 (1984). TLC of curcumine in turmeric root powder and spice-mixtures on silica with chloroform - acetic acid 8:2. Detection by UV or spraying with boric acid - oxalic acid - reagent. Fluorometry after spraying.