59th Indian Pharmaceutical congress C-305, 297, (2007). Phytoconstituents of mature and immature tubers of Ipomoea mauritiana (methanolic and aqueous extracts) have been studied. HPTLC on silica gel with chloroform - methanol - formic acid 6:3:1. Detection by spraying with vanilin - sulphuric acid reagent. Densitometric evaluation at 365 nm. Mature tubers were found to contain higher concentration of phytoconstiuents than immature tubers.

J. Sep. Sci. 30, 2167-2172 (2007). HPTLC of clozapine in human serum on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 290 nm. Linearity was between 10 and 100 ng/zone. The intra-assay variation was between 2.10 and 3.33 % (n=5) and inter-assay variation was between 2.67 and 4.44 % (n=9). The limits of detection and quantification were 0.03 and 0.05 ng/µL, respectively. Recovery was between 97.0 and 99.0 %, and selectivity regarding matrix was given.

CBS 98, 2-4 (2007). HPTLC of amino-3-propan-1-ol, a degradation product of dexpanthenol, on silica gel with ethanol - water - acetic acid 16:3:1. To the mobile phase the derivatization reagent was added, i.e. 0.5 g ninhydrin were dissolved in 100 mL solvent mixture. Development in the horizontal developing chamber from both plate sides over 40 mm. Detection by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 486 nm. The hRf value of amino-propanol was 50. The mean repeatability was 4.9 % at 5 different concentration levels. The relative standard deviation of the intermediate precision (n=9) was 5.7 %. The limit of detection and quantification was 4.5 µg/mL and 15 µg/mL, respectively (related to the application volume of 2 µL). Recovery (n=15) was 102 %.

J. Planar Chromatogr. 20, 275-277 (2007). HPTLC of bergenin and gallic acid on silica gel with ethyl acetate - formaldehyde - acetic acid - water 80:1:2:1 in a saturated twin-trough chamber. Quantitation was performed by scanning at 260 nm in absorption mode.

J. Planar Chromatogr. 21, 177-181 (2008). TLC of azithromycin on silica gel with chloroform - ethanol - ammonia 30:70:1. Detection by spraying with sulfuric acid - ethanol 1:4 followed by heating at 120 °C for 5 min. Quantitation by densitometry at 483 nm.

Pharmazie 63, 659-662 (2008). HPTLC and TLC of 1’-acetoxychavicol acetate on silica gel and RP-18. Detection under UV 254 and 365 nm, and after spraying with 1 % cerium sulfate/10 % sulfuric acid, and anisaldehyde reagent (5 mL acetic acid, 25 mL concentrated sulfuric acid, 425 mL ethanol, and 25 mL water).

Ind. J. Pharm. Sci. 69 (5), 684-686 (2007). HPTLC of lansoprazole and domperidone in tablet formulations on silica gel with n-butanol - acetic acid - water 186:5:10. Densitometric analysis in absorbance mode at 288 nm. The calibration covered the range of 100 to 500 ng/spot for both substances. The hRf value of lansoprazole and domperidone was 78 and 21, respectively. The limit of detection and quantification for lansoprazole was 10 and 40 ng/spot and for domperidone 30 and 65 ng/spot, respectively. The method is suitable for routine quality control.

Ind. J. Pharm. Sci. 69 (6) 841 - 844 (2007). HPTLC of artesunate on silica gel with toluene - ethyl acetate - acetic acid 20:80:2. Detection by treatment with vanillin reagent (1 % vanillin in 5 % ethanolic sulphuric acid) leads to pink zones which are stable for more than a day. Quantitative determination by absorbance measurement at 520 nm. The hRf value for artesunate was 44. Linearity was between 100 and 600 ng per spot. Recovery (by standard addition method) was 98.9 - 99.9 % for tablets and injections.