CBS 105, 2-4 (2010). TLC and HPTLC of reaction samples from small molecule lead development, on silica gel with mixtures of methanol and dichloromethane/ethyl acetate or ethyl acetate and heptane/cyclohexane (ratios depending on the compound mixtures). Detection with primuline or berberine reagent. Direct elution into the MS with the TLC-MS interface. Substances not detected by DAD can successfully be measured by ELSD detection coupled to TLC.

62nd Indian Pharmaceutical Congress Abstract No. F-233 (2010). TLC of metoprolol and ramipril on silica gel with methanol – toluene – ethyl acetate – ammonia 25:30:50:7. Quantitative determination by absorbance measurement at 209 nm. The hRf values of metoprolol and ramipril were 67 and 37, respectively. The reliability of the method was assessed by evaluation of linearity (2-12 µg/band for metoprolol and 0.2-1.2 µg/band for ramipril).

62nd Indian Pharmaceutical Congress Abstract No. F-264 (2010). TLC of lamivudine, zidovudine and nevirapine on silica gel with chloroform – methanol 9:1. The hRf values were 7, 46 and 77 for lamivudine, zidovudine and nevirapine, respectively. Quantitative determination by absorbance measurement at 265 nm. The method was linear in the range of 90-210 µg/band, 180-240 µg/band and 120-280 µg/band for lamivudine, zidovudine and nevirapine respectively.

62nd Indian Pharmaceutical Congress Abstract No. F-381 (2010). TLC of citicoline sodium on silica gel with chloroform – methanol – water 3:7:3. The hRf value was 53. Quantitative determination by absorbance measurement at 280 nm. The results of the method were comparable with the results of a RP-HPLC method.

J. of Chromatogr. Sci. 49, 129-135 (2011). TLC on silica gel with ethyl acetate – chloroform – formic acid – triethyl amine 70:30:1:1. Detection and quantification by densitometry. Good correlation between the integrated peak area of the studied drugs and their corresponding concentrations was found in different ranges.

Acta Chromatographica 20(3), 423-437 (2008). HPTLC of rupatadine fumarate on silica gel with toluene – methanol – triethylamine 20:5:1. The hRf value was 61. Quantitative determination by absorbance measurement at 264 nm. The linearity was in the range of 400–1400 ng/band (r=0.9992). The limits of detection and quantitation were 67 and 202 ng/band, respectively. Moreover, rupatadine fumarate was subjected to acid and alkaline hydrolysis, oxidation, and photochemical and thermal degradation and underwent degradation under all these conditions. The method proved to be repeatable, selective, and accurate for the analysis of the drug by statistical analysis, and is able to separate the degradation products from the drug.

Rapid Commun. Mass Spectrom. 25, 2275-2282 (2011). The authors explored the possibility of the desorption at an angle scanning analysis of surfaces from direct analysis in real time mass spectrometry (DART-MS), including the coupling of planar chromatography with DART-MS. A method for the visualization of the impact region of the DART gas stream was developed, as well as the DART-MS detectability of liquids was increased, improving the capabilities of DART-MS in trace analysis.

J. of China Pharm. 19 (23), 24-25 (2010). TLC of the extracts of traditional Chinese formulations and some health-care foods on silica gel with dichloromethane - acetone 4:1. Detection under UV 254 nm. This TLC method was better than the previously used one with the mobile phase dichloromethane - diethyl ether - methanol - water 385:60:15:2 and detection reagent 2 % tetrazolium solution - 2N NaOH - methanol 3:10:5. Identification of dexamethasone, prednisone, prednisolone acetate, prednisone acetate, cortisone acetate, hydrocortisone, betamethasone, hydrocortisone acetate, dexamethasone acetate, fluocinolone acetonide acetate by comparison with the standards. The improved procedure proved to be simple, fast, robust and suitable for quick screening of 10 glucocorticoids illegally added to some traditional Chinese formulations and health-care foods.