J. Planar Chromatogr. 30, 199-204 (2017). HPTLC of sophoridine (1), matrine (2), and sophocarpine (3) in S. alopecuroides on silica gel with toluene – acetone – methanol – ammonia – water 80:30:2:5:80. Detection by spraying with bismuth potassium iodide reagent (1:1 mixture of 0.85 g of bismuth nitrate with 10 mL of glacial acetic acid and 40 mL of water, heated for dissolution, and 8 g of potassium iodide in 30 mL of water). Quantitative determination by absorbance measurement at 500 nm. The hRF values for (1) to (3) were 22, 56 and 65, respectively. Linearity ranged from 415-2491 ng/zone for (1), 424-2547 ng/zone for (2) and 410-2460 ng/zone for (3). The intermediate precision was <5 % (n=3). Recovery rate ranged from 97.8 to 98.9 % for (1), 98.3 to 100.9 % for (2) and 99.1 to 101.2 % for (3).

Chinese J. Hosp. Pharm. 35 (4), 288-292 (2015). Zhichuang Xunxi Ye fumigant is a herbal TCM for the treatment of hemorrhoids. For quality control, TLC of its extracts, (1) for Leatherleaf Mahonia Stem, on silica gel preconditioned with ammonia vapor and developed with toluene – ethyl acetate – methanol – isopropanol - ammonium hydroxide 12:6:3:3:1, detection at UV 366 nm; (2) for Radix sophorae flavescentis, on silica gel impregnated with 2 % NaOH, with (A) toluene – acetone – methanol 16:6:1, (B) the upper phase of toluene – ethyl acetate – methanol – water 2:4:2:1 under 10 ºC overnight, detection by spraying with 5 % potassium iodobismuthate in HCl – water 1:200 and evaluation in daylight; (3) for Phellodendron Chinense Schneid., on silica gel with chloroform - methanol – water 30:15:4, detection by spraying with 5 % potassium iodobismuthate in HCl – water 1:200 and evaluation in daylight. Quantification of berberine chloride hydrate and matrine by HPLC.

J. Liq. Chromatogr. Relat. Technol. 40, 863-871 (2017). Comprehensive literature review during 1983-2016 of the application of TLC for the identification, separation, and determination of surfactants, including the use of green solvents and ionic liquids.

J. Planar Chromatogr. 30, 502-509 (2017). HPTLC of chlorogenic acid (1), gallic acid (2), caffeic acid (3), quercetin (4), p-coumaric acid (5) and kaempferol (6) in the fresh leaves, flowers, pods, and dried pods and seeds of M. oleifera on silica gel with toluene – ethyl acetate – formic acid 14:10:1. Quantitative determination by absorbance measurement at 282 nm. The hRF values for (1) to (6) were 1, 31, 41, 45, 49 and 52, respectively. Linearity was between 0.1 and 1.5 μg/zone for (1) to (6). LOD and LOQ were 142 and 382 ng/μL for (1), 126 and 343 ng/μL for (2), 114 and 410 ng/μL for (3), 116 and 301 ng/μL for (4), 125 and 356 ng/μL for (5) and 110 and 345 ng/μL for (6), respectively. The intermediate precision was <3 % (n=6). Average recovery was 96.2 % for (1), 97.9 % for (2), 97.3 % for (3), 96.5 % for (4), 97.1 % for (5) and 96.2 % for (6).

Phytochem. Anal. 28, 125-131 (2017). HPTLC of the dried fractions of Morus alba wood on silica gel with dichloromethane – methanol 22:3, and to achieve better resolution for the high activity fractions with dichloromethane – methanol 83:17. Qualitative determination under UV 254 and 366 nm and detection with sulfuric acid reagent. Partial least squares-regression was performed to discover the substances that were most correlated to bioactivity.

J. Chromatogr. A 1532, 198-207 (2018). Development of a method to analyze the efficiency of a diverse set of natural deep eutectic solvents (NADES) for the extraction of compounds of interest from two model plants, Ginkgo biloba and Panax ginseng. HPTLC on silica gel with toluene – ethyl acetate – acetone – methanol 50:25:25:3 for ginkgolides in Ginkgo biloba leaves; with ethyl acetate – acetic acid – formic acid – water 100:11:11:27 for phenolics in Ginkgo biloba leaves; with toluene – ethyl acetate – acetic acid 40:10:1 for ginkgolic acids in Ginkgo biloba leaves; with chloroform – ethyl acetate – methanol – water 15:40:22:10 for ginsenosides in Panax ginseng leaves and stems. The six different NADES were combinations of two or three compounds mixed in defined molar ratios, e.g. malic acid – choline chloride 1:1, malic acid – glucose 1:1, choline chloride – glucose 5:2, malic acid – proline 1:1, glucose – fructose – sucrose 1:1:1 and glycerol – proline – sucrose 9:4:1. Processing the data obtained by multivariate data analysis showed differences between the extracts. Discussion of the foreground of application of NADES in green chemistry and the advantages of NADES as green solvents used in novel green products for the food, cosmetics and pharmaceuticals.

Biochem. Biophys. Res. Commun. 491, 265-270 (2018). HPTLC of nitrobenzoxadiazole (NBD)-labeled lipids in the reaction mixture of ceramide glucosyltransferase (GlcT-1) on silica gel with chloroform – methanol – water 65:25:4. NBD-labeled lipids were visualized by UV-B illumination. The amount of C6-NBD-glucosylceramide (GlcCer) generated was quantified by TLC image processing. The method was used to study the effects of JAK/STAT3 pathway inhibitors on GlcT-1 activity.

J. Chil. Chem. Soc. 63, 3834-3840 (2018). TLC of clofarabine on silica gel with toluene – methanol 4:1. Quantitative determination by absorbance measurement at 266 nm. The hRf value for clofarabine was 34. Linearity was in the range of 50-1000 ng/zone. The intermediate precision was below 2 % (n=3). The LOD and LOQ were 17 and 53 ng/zone, respectively. Recovery was between 99.6 and 100.6 %.