Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

Page
      130 142
      Bioassay-guided identification of α-amylase inhibitors in herbal extracts
      Snezana AGATONOVIC-KUSTRIN*, E. KUSTRIN, V. GEGECHKORI, D. W. MORTON (*Department of Pharmaceutical and Toxicological Chemistry, Institute of Pharmacy, Sechenov University, Moscow, Russia, and School of Pharmacy and Biomedical Sciences, La Trobe Institute for Molecular Sciences, La Trobe University, Bendigo, Australia; s.kustrin@latrobe.edu.au)

      J Chromatogr A, 1620, 460970 (2020). Samples were ethyl acetate extracts of Lavandula angustifolia herb and flowers and of aerial parts of other Lamiaceae (Ocimum basilicum, Origanum vulgare, Thymus vulgaris, Rosmarinus officinalis, Salvia officinalis), as well as standards. HPTLC on silica gel (pre-washed with methanol and heated 30 min at 105 °C) with n-hexane – ethyl acetate – acetic acid 70:27:3. Documentation at UV 254 nm and 365 nm and white light before and after A) derivatization with anisaldehyde – sulfuric acid reagent, followed by 10 min heating at 110 °C; B) spraying with DPPH• (0.2 % in methanol), followed by 30 min incubation in the dark; C) α-amylase inhibition assay by immersion into enzyme solution, incubation 30 min at 37 °C, immersion into substrate solution (starch 1 % in water), incubation 20 min at 37 °C and immersion into Gram’s iodine solution for detection (inhibition zones appear blue on white background). Quantification was performed on pictures using image processing software, and expressed as equivalents to the respective standards used for calibration curves: A) β-sitosterol (LOQ 1.5 µg/band), B) gallic acid (LOQ 60 ng/band), C) acarbose (LOQ 8 µg/band). An amylase inhibiting zone (hRF 68) present in all samples (except L. angustifolia), scraped from untreated plates and washed with ethyl acetate, was tentatively identified by ATR-FTIR analysis as oleanolic acid (pentacyclic triterpene).

      Classification: 4e, 15a, 32e
      130 004
      Identification of acetylcholinesterase inhibitors in water by combining two-dimensional thin-layer chromatography and high-resolution mass spectrometry
      Lena STÜTZ*, W. SCHULZ, R. WINZENBACHER (*Laboratory for Operation Control and Research, Zweckverband Landeswasserversorgung, Langenau, Germany; stuetz.l@lw-online.de)

      J Chromatogr A, 1624, 461239 (2020). Samples were chemical standards of acetylcholinesterase (AChE) inhibitors (azamethiphos, caffeine, donepezil, galanthamine, methiocarb-sulfoxide, paraoxon-ethyl) and of neurotoxic compounds, as well as drinking or contaminated water samples enriched through solid phase extraction. HPTLC on spherical silica gel (pre-washed twice by 20 min immersion in isopropanol, heated 20 min at 120 °C before and after pre-washing with acetonitrile). First separation (preparative TLC) with automated multiple development (16 steps). Effect-directed analysis for AChE inhibitors by immersion (speed 5 cm/s, time 1 s) into enzyme solution, incubation 5 min at 37 °C and immersion into substrate solution (indoxyl acetate 2 % in methanol); visualization under UV 366 nm. Active zones from untreated layers were eluted through the oval head of a TLC-MS interface to a second plate for a second separation with a panel of other mobile phases. Bands of interest were eluted from the second layer with water through the oval elution head of the TLC-MS interface pump, into a RP18 liquid chromatography guard column, followed by a quadrupole time-of-flight mass spectrometer. Full scan mass spectra (m/z 100–1200) were recorded in negative and positive modes using electrospray ionization (and collision-induced dissociation for MS2). Among the water contaminants, lumichrome (riboflavin photolysis product), paraxanthine and linear alkylbenzene sulfonates were identified as AChE inhibitors.

      Classification: 3d, 4d, 4e, 22, 29b, 35d, 37c
      130 005
      Multiobjective optimization of microemulsion – thin layer chromatography with image processing as analytical platform for determination of drugs in plasma using desirability functions
      Noura H. ABOU-TALEB*, D. T. EL-SHERBINY, N. M. EL-ENANY, H. I. EL-SUBBAGH (*Medicinal Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; nourahemdan@yahoo.com)

      J Chromatogr A, 1619, 460945 (2020). Samples were lamotrigin as standard, or extracted with an oil-in-water microemulsion (10 µL butyl acetate, 4 mL n-butanol, 925 mg sodium dodecyl sulphate, 8.6 mL water) either from patients’ raw plasma (for separation from blood proteins) after spiking, or from commercial tablets dissolved in methanol. TLC on silica gel with a water-in-oil microemulsion of 9 mL butyl acetate, 1 mL n-butanol, 250 mg sodium dodecyl sulphate, 250 µL water. Both optimal microemulsions were predicted using Taguchi orthogonal array and Plackett-Burman design. Evaluation in UV 254 nm, quantification from the digital picture using four image processing software programs. For lamotrigin (hRF 24), limits of quantification were 170 ng for pure drug and 10 ng for spiked plasma. Linearity (in range 20–200 ng/spot) was directly obtained for the calibration curve in spiked plasma; however, for pure drug, linearity was obtained only when using log values of the calculated densities (300–3000 ng/spot).

      Classification: 3a, 3d, 5c, 23e, 32c
      130 006
      Thin-layer chromatography with eutectic mobile phases – preliminary results
      Danuta RAJ* (*Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Wroclaw, Poland; danuta.raj@umed.wroc.pl)

      J Chromatogr A, 1621, 461044 (2020). Samples were five isoquinoline alkaloids (berberine, chelerythrine, chelidonine, coptisine, sanguinarine) either as standard mixture or present in a Chelidonium majus (Papaveraceae) herb extract obtained with HCl 0.05 M in methanol. Separation on TLC and HPTLC silica gel layers with a screening of mobile phases consisting of eutectic mixtures of chemicals and/or phytochemicals. These homogenous stable liquids called DES (deep eutectic solvents) were obtained either simply by mixing, or by mixing followed by heating at 50°C, or by mixing with water for dissolution followed by dehydratation through rotary evaporation. For polarity adjustment, the DES phases were tested pure or diluted with acetone, chloroform, diethyl ether, methanol, or water. Visualization under UV 366 nm. The best separation was obtained with menthol – phenol in equimolar mixture, with 35 % methanol added (hRF values of the selected alkaloids were 33, 39, 79, 20 and 52, respectively).

      Classification: 22, 32e
      130 001
      Separation and detection of apricot leaf triterpenes by high-performance thin-layer chromatography combined with direct bioautography and mass spectrometry
      Ágnes M. MÓRICZ*, P. G. OTT (*Plant Protection Institute, Centre for Agricultural Research, 1022 Budapest, Hungary; moricz.agnes@agrar.mta.hu)

      J Chromatogr A, 1675, 463167 (2022). Samples were ethanol extracts (and their flash chromatography fractions) of Prunus armeniaca leaves (Rosaceae), as well as betulinic, linolenic, maslinic (= crataegolic), oleanolic, ursolic acids and pygenic acids A (= corosolic acid) and B b as standards. When needed, to improve separation of triterpenoids, reversible pre-chromatographic derivatization was performed in situ by applying 10 µL iodine solution (2 % in chloroform) either before development on the deposit band, or for 2D-HPTLC after a first separation up to 60 mm and before a second orthogonal separation. Layers were covered 10 min with glass sheet after iodine application, and then dried 1 min under cold air stream. HPTLC on silica gel with chloroform – ethyl acetate – methanol 20:3:2, 85:9:6, or 15:2:3), followed by 5-10 min drying under cold air stream (eliminating iodine completely). Post-chromatographic derivatization by immersion (time 2 s, speed 3 cm/s) into vanillin – sulfuric acid (40 mg and 200µL, respectively, in 10 mL ethanol), followed by heating 5 min at 110 °C. Antibacterial effect-directed analysis was performed by immersion (time 8 s) into Bacillus subtilis suspension, followed by 2 h incubation at 37 °C, immersion in MTT solution and 30 min incubation at 37 °C. Active bands were eluted from layer with methanol through the oval elution head of a TLC-MS interface pump, into a single quadrupole mass spectrometer to record full scan mass spectra (m/z 200–1200 in both modes) using electrospray ionization (interface temperature 350°C, heat block temperature 400°C, desolvation line temperature 250°C, detector voltage 4.5kV). Five triterpenoids were identified: betulinic, corosolic, maslinic, oleanolic and ursolic acids, acid, as well as two fatty acids: linolenic and palmitic acid.

      Classification: 4e, 11a, 15a, 32e
      130 114
      Simultaneous ultra‑sensitive analysis of tamsulosin hydrochloride and tolterodine tartrate binary mixture in their dosage form via high‑performance thin‑layer chromatography with fluorimetric detection
      M. RIZK, Z. MAHMOUD*, M. AZAB (*Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Cairo 11795, Egypt, Zainab.MansourMahmoud@pharm.helwan.edu.eg)

      J. Planar Chromatogr. 35, 509-517 (2022). HPTLC of tamsulosin hydrochloride (1) and tolterodine tartrate (2) binary mixture on silica gel with ethyl acetate - n-hexane - diethylamine 9:3:1. Quantitative determination by absorbance measurement at 225 nm. The hRF values for (1) and (2) were 40 and 85, respectively. Linearity was between 10 and 200 ng/zone for (1) and 100 and 900 ng/zone for (2). The LOD and LOQ were 3 and 8 ng/zone for (1) and 22 and 66 ng/zone for (2), respectively. Average recovery was 100.1 % for (1) and 100.7 5 for (2).

      Classification: 32a
      130 115
      ICH and US‑FDA validated HPTLC methods with greenness assessments for the assay of mixtures prescribed in stroke prophylaxis: application to pharmaceutical preparations and human plasma
      M. HAMDY*, M. KORANY, S. EBIED, R. HAGGAG (*Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt, mohamed.hamdy@pua.edu.eg)

      J. Planar Chromatogr. 35, 519-532 (2022). HPTLC of binary mixtures of the novel oral anticoagulants (NOACs) apixaban (1), edoxaban tosylate (2) and rivaroxaban (3) with the lipid-lowering statin, rosuvastatin calcium (4) on silica gel with toluene - ethyl acetate - methanol - 25 % ammonia 35:45:20:2 (method 1) for the three mixtures, and methanol - 25 % ammonia 199:1 (method 2) for (2)/(3) mixture only. Quantitative determination by absorbance measurement at 291 nm. The hRF values for (1) to (4) were 65, 20, 75 and 10 using method 1, and 40 for (2) and 90 for (4) using method 2. Linearity was between 5 and 45 µg/mL for (1) to (4). Interday and intra-day precisions were below 3 % (n=6). The LOD and LOQ were 0.1 and 0.4 µg/mL for (1), 1 and 4 µg/mL for (2) and (3), 0.4 and 1 µg/mL for (4) using method 1, and 1.4 and 4.7 µg/mL for (2) and 0.4 and 1.2 µg/mL for (4) using method 2. Average recovery was between 97.6 and 102.9 % for (1) to (4).

      Classification: 32a
      130 116
      Agar‑free high‑performance thin‑layer chromatography–bioautography method for the qualitative estimation of α‑amylase inhibitor in Syzygium cumini seed extract and formulation
      S. BHUJBAL*, B. CHAWALE, M. KALE, R. BHUJBAL (*Department of Pharmacognosy, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Sant. Tukaram Nagar Pimpri, Pune, Maharashtra 411018, India, santosh.bhujbal@dypvp.edu.in)

      J. Planar Chromatogr. 35, 501-507 (2022). HPTLC of β-sitosterol the seeds of Syzygium cumini on silica gel with toluene - ethyl acetate - methanol - glacial acetic acid 6:3:2:1. Detection by dipping into anisaldehyde sulfuric acid solution for 3 s, followed by heating at 80-100 °C for 2-3 min. The assessment of α-amylase inhibitors was performed by dipping into enzyme solution (10 mg α-amylase enzyme in 20 mL of sodium acetate buffer and stored at 2–8 °C) for 2–3 seconds, followed by 90 min humidification in a desiccator and the dipping into a 1 % starch solution as a substrate and put in a humid environment for additional 20–30 min to allow the enzyme–substrate interaction to occur. After a 2–5-min time, the plate was washed or dipped in Gram’s iodine blue, which revealed anti-diabetic activity as blue stains on a white background. The hRF value for β-sitosterol was 87. Further analysis by high‑resolution mass spectrometry.

      Classification: 13c
Page