Abstract No. C-291, 61st IPC (2009). Quantitative determination of curcumin in the marketed herbal product Rheumax (Herbajules rumatis) containing extracts of Curcuma longa, Boswellia serrata, Tinospora cordifolia and Vitex negundo by TLC on silica gel with chloroform - methanol 37:3. The hRf value of curcumin was 59. Quantitative absorbance measurement at 430 nm. The linear regression between 100 and 500 ng/zone showed R2 of 0.99984 and RSD of 1.58 % for curcumin.

Journal Pharmacy Research 2(9), 1542-1546 (2009) TLC on silica gel with n-hexane - ethyl acetate - glacial acetic acid 75:25:2. The hRf value was 38. Densitometric evaluation at 225 nm. The method was linear over a concentration range of 100-350 ng/band. The average recovery was 100.2 %. The samples were subjected to different stress conditions (acid, base, oxidative, heat) and all the degradation products were well resolved from the main compound. The method is suitable for stability studies and routine quality control.

Biochem. Biophys. Res. Commun. 399, 150-154 (2010). HPTLC of the lipids from mouse primary macrophages on silica gel with butanol - acetic acid - water 3:1:1. Detection by imaging using Fujifilm intelligent dark box in SYBR green fluorescent light. Quantitative determination with a TLC plate imaging software.

Asian Journal of Chemistry 22(4), 3283-3291 (2010). TLC of vitamins B1, B6, and B12 in formulations on bentonite-coated TLC plates (0.3 mm) with iso-butylalcohol - methanol - chloroform - acetic acid - ammonia 75:10:35:4:5. The hRf value of vitamin B1 was 26, of B6 37 and of B12 68. The method was linear in the range of 500-4000 ng/band for all three vitamins. Densitometric quantification at 275 nm for B1, B6, and B12 and at 525 nm for B12.

J. Planar Chromatogr. 23, 265-269 (2010). TLC of the epimers of cefaclor on silica gel (impregnated with beta-cyclodextrin by development with 1:9 aqueous beta-cyclodextrin solution - methanol) with chloroform - ethyl acetate - glacial acetic acid - water 4:4:4:1 with chamber saturation. Chromatograms were developed at 5 °C and dried at room temperature. Quantitative determination by absorbance measurement at 274 nm. The limit of detection was 0.24 and 0.27 µg/band for the epimers at hRf 26 and 33, respectively. The limit of quantification was 0.74 and 0.83 µg/band, respectively. The recovery was between 100.0 and 100.8 % and the precision between 0.7 and 1.7 %.

J. Serb. Chem. Soc. 74(6), 677-688 (2009). An NP-TLC method has been reported to study the lipophilicity of 5 ACE inhibitors (lisinopril, quinapril, fosinopril, enalapril, cilazapril and their metabolites). TLC on silica gel with several non-aqueous mono and binary solvent systems. Binary mobile phases demonstrated a decrease in hRf values of all 5 ACE inhibitors, i.e. increased retention with increased amounts of the less polar component in the mobile phase. Metabolites usually exhibited stronger retention, i.e. lower hRf values than the corresponding ACE inhibitor. This is probably due to a different interaction with silica gel because of two carboxylic groups in the structure of the metabolites, whereas ACE inhibitors contain only one carboxyclic group. Results obtained on NP-TLC were compared with those by RP-TLC and no significant difference was found regarding lipophilicity.

Revista Iberica de Parasitologia 65 (1-4), 21-36 (2005). Review on the TLC literature in helminthology from 1996 to 2004. Principles and practices of modern TLC for the analysis of lipids, amino acids, carbohydrates and pigments in helminths such as various species of trematodes, cestodes and nematodes are described.

62nd Indian Pharmaceutical Congress Abstract No. F-239 (2010). TLC of cefixime and ambroxol (extracted from human plasma with acetonitrile - methanol 3:1, centrifuged and dried at 40 °C, then dissolved in methanol) on silica gel with acetonitrile – methanol – triethylamine 41:5:4. The hRf values were 27 and 54 for cefixime and ambroxol. Quantitative determination by absorbance measurement at 254 nm. The recovery from plasma was in the range of 69.5-74.4 % for ambroxol and 83.5-87.9 % for cefixime.