J. Planar Chromatogr. 17, 286-289 (2004). HPTLC of isoquercitrin, avicularin, rutin, apigenin 7-glucoside, naringin, and hesperidin on silica gel with ethyl acetate - methanol - formic acid 90:10:1. Detection under UV light at 254 nm and by spraying with 1 % methanolic diphenylboric acid beta-ethylamine ester, followed by spraying with 5 % ethanolic polyethylene glycol 4000. Quantitative determination by densitometry at 254 nm. Report of the possibilities and advantages of HPTLC for investigation of hydrolysis.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(2), 246-248 (2005). TLC of extracts prepared by different processing technology on silica gel with 1) petroleum ether (60 - 90ºC) - chloroform - methanol 10:3:2; 2) chloroform - methanol 5:1; 3) benzene - glacial acetic acid 4:1. Detection 1) under UV 254 nm; 2) by spraying with 1 % vanillin - H2SO4 solution and heating at 105 ºC. Identification by fingerprint technique. Quantification of gastrodine by HPLC. Discussion of using the procedures for monitoring the preparation process and the quality control of the medicine products.

IPC 56th 2004, Abstract No. G-18. Curculigo Orchioides (Amary llidaceae) is used in various ayurvedic formulations. The rhizomes contain about 5.78 % total phenolis, gallic acid being the major component of the alcoholic extracts. HPTLC of gallic acid on silica gel with toluene - ethyl acetate - glacial acetic acid 25:15:1. The Rf valueof gallic acid was 0.19, the linearity range of 150-750 ng/spot. Rhizomes were found to contain 2.54 % gallic acid, formulations contained 5.13 % gallic acid, recovery was 99.5 %.

Part I. J. Chromatogr. A 1007 (1-2), 145-155 (2003). A quantitative structure-activity relationship analysis of H1-antihistamine activity and chromatographic data of 2-[2-(phenylamino)thiazol-4-yl]ethanamine; 2-(2-benzyl-4-thiazolyl)ethanamine; 2-(2-benzhydrylthiazol-4-yl)ethylamine derivative; 2-(1-piperazinyl- and 2-(hexahydro-1H-1,4-diazepin-1-yl)benzothiazole derivatives were collected. Silica gel RP2, impregnated with solutions of selected amino acid mixtures (-Asp, -Asn, -Thr and -Lys), were used in two developing solvents as hH1R antagonistic interaction models. Using regression analysis, the relationships between chromatographic and biological activity data were found. The correlations obtained in regression analysis for the examined thiazole and benzothiazole derivatives with H1-antihistamine activity [pA2(H1)] represent their interaction with all the proposed biochromatographic models (S1–S7). Some of the calculated equations can be applied to predict the pharmacological activity of new drug candidates. The best multivariate relationships useful in predicting the pharmacological activity of thiazole and benzothiazole derivatives were obtained on RP2 phase with acetonitrile - methanol - buffer 2:2:1. The log P values of particular compounds are extremely important for this kind of activity.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27 (3), 275-278 (2005). TLC of the extracts on silica gel with 1) chloroform - ethyl acetate - methanol - water 15:40:22:10; 2) n-butanol - ammonia - ethanol 5:2:1; 3) the lower phase of chloroform - ethyl acetate - methanol - water 8:8:3:2. Detection 1) by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC until the spots appear; 2) by spraying with 5 % potassium iodobismuthate solution. Identification by fingerprint technique. Quantification of ginsenoside Rb1 by HPLC.

Abstract G-33, IPC (2005). HPTLC of epalrestat in plasma on silica gel with ethyl acetate - toluene - acetic acid 30:20:1. Nitrofuranloin was used as internal standard. Quantitative determination by absorbance measurement at 390 nm. Linearity was obtained the range of 0.01-0.20 µg/mL with recovery of 99-107 %. The method was validated as per ICH guidelines.

J. Planar Chromatogr. 18, 403-404 (2005). HPTLC of antioxidants (Irgafos 168, Irganox 1010, Irganox 1078, Irganox 1330, and BHT) and hexane extracts of polymer granules on silica gel (prewashed with chloroform -methanol 1:1) in an unsaturated twin-trough chamber with hexane - methanol 1:4. Quantitative determination of Irganox 1078 by absorbance measurement at 254 nm.

J. AOAC Int. 89, 619-623 (2006). HPTLC of phyllanthin, hypophyllanthin, gallic acid, and ellagic acid on silica gel with toluene - ethyl acetate - formic acid 6:2:1 at 25 +/- 2 °C and 40 % relative humidity. Quantitation by densitometry at 280 nm. The method was validated for precision (0.54, 0.93, 0.08, and 1.06 %, respectively), repeatability (1.01, 0.79, 0.98, and 1.06 %, respectively), and accuracy, determined by a recovery study at 3 different levels (99.09 %, 99.27 %, 98.69 %, and 100.49 %, respectively).