J. Chinese Trad. Patent Med. (Zhongchengyao) 26 (1), 19-23 (2004). TLC on silica gel in a basic developing system containing diethylamine. Detection by iodine vapour. Identification by standard comparison. Quantitative determination by densitometry at 340 nm. Validation of the method by investigation of the procedures, including sample preparation, mobile phase, visualization, scanning wavelength, linearity range (0.28-1.65 µg, r=0.9991), precision (RSD <1.4 % within plate and <2.4 % plate-to-plate), resolution, repeatability, reproducibility, and recovery (87.2 %-99.7 % for different medicine). Comparison of the results with those obtained by HPLC.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (7), 547-549 (2004). TLC on silica gel with 1) chloroform - methanol - water 13:7:2; 2) chloroform - methanol - ethyl acetate - formic acid 20:8:4:1; 3) petroleum ether - ethyl acetate - formic acid 15:5:1; 4) chloroform - methanol - water 14:5:1. Detection 1) by spraying with 10% H2SO4 in ethanol and heating at 105 ºC; 2) by spraying with 5 % vanillin-H2SO4 solution and heating at 105 ºC; 3) under UV 365 nm. Identification by finger print technique. Quantitation of puerin by HPLC.

J. Chinese Trad. Patent Med. (Zhongchengyao) 26 (1), 23-26 (2004). TLC on silica gel with 1) chloroform - methanol - water 28:10:1, 2) n-hexane - ethyl acetate 9:1, 3) chloroform - methanol 5:1, 4) petroleum ether - diethyl ether 1:1. Detection 1) under UV 365 nm, 2) by spraying with 5 % vanillin in H2SO4 solution. Identification by fingerprint techniques. Quantitative determination of puerarin by HPLC.

J. Planar Chromatogr. 17, 14-17 (2004). HPTLC of oxindole alkaloids on silica gel, Diol-, CN-, NH2-, and RP-18-phases. Normal-phase TLC with 1) ethyl acetate - methanol - water 200:27:20; 2) ethyl acetate - methanol - water - acetic acid 100:2.7:5:3; 3) ethyl acetate - methanol - water - formic acid 100:2.7:5:3; 4) ethyl acetate - isopropanol - ammonia 100:2:1. RP TLC with water - methanol 7:3 containing 0.25 % ammonia; development in horizontal chamber (sandwich configuration) at room temperature. Two-dimensional TLC with mobile phase 2 in the first direction and, after drying, with mobile phase 3 in the second direction. Evaluation under UV 254 nm and after derivatization with iodine reagent or with Dragendorff reagent. TLC separations were documented by scanning. Silica and cyanopropyl layers with acidic aqueous mobile phases gave the best separation.

Indian Drugs 41 (3), 160-164 (2004). Combinations of ofloxacin and tinidazole (1:3) are available as tablet dosage forms. Stability indicating HPTLC of ofloxacin and tinidazole on silica gel with dioxan - ethyl acetate - toluene - acetic acid - water 5.5:5:3.2:2:2 . Quantification by absorbance measurement at 307 nm. The Rf value of ofloxacin and tinidazole was found to be 0.12 and 0.85 respectively. Recovery was 99.85 % and 99.45 % for ofloxacin and tinidazole respectively. The proposed method was quantitatively evaluated in terms of calibration of concentration range, stability, and accuracy. It can be adopted for routine analysis of the formulation.

Indian J. Pharm. Sci. 66 (3), 278-282 (2004). HPTLC on silica gel with chloroform - acetonitrile - toluene - acetate buffer (pH 6.0) 50:40:10:3. Quantitative determination by absorbance measurement at 266 nm. The method was validated in terms of linearity, accuracy, precision, and specificity. The limit of detection and the limit of quantification were found to be 50 ng/spot and 100 ng/spot respectively. A simple, precise, accurate and rapid HPTLC method has been developed and validated for the simultaneous determination of cefuroxime axetil and probenecid in combined dosage form.

Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (6), 449-452 (2004). TLC of Qixuekang oral liquid extract on silica gel with 1) chloroform - ethyl acetate - methanol - water 15:40:22:10; 2) chloroform - methanol - water 28:10:1. Detection 1) by spraying with 10 % H2SO4 in ethanol and heating; 2) under UV 365 nm. Identification by fingerprint techniques. Quantification of notoginsenoside R1 by HPLC with method validation.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(1), 94-96 (2005). HPTLC on silica gel with 1) n-butanol - glacial acid - water 19:5:5; 2) petroleum ether (30-60 ºC) - formic acetate - formic acid 15:5:1; 3) chloroform - ethyl acetate - methanol - formic acid 200:25:50:1. Detection 1) by spraying with 0.5 % ninhydrin in ethanol; 2) under UV 365 nm; 3) by spraying with vanillin - H2SO4 solution and heating. Identification by fingerprint technique. Quantification of emodin by densitometry at 440 nm. Validation of the method by investigation of linearity (0.1 µg - 0.5 µg, r = 0.998); precision (RSD = 3.8 %, n = 15 within plate and RSD = 3.2 %, n = 5 plate to plate); reproducibility of five time assay towards the same sample (RSD = 4.4 %); standard addition recovery (99.5 %, RSD = 2.2 %, n = 5). The results for some real life samples are given.