Chromatographia 70 (1-2), 305-308 (2009). Description of color channel selection, three-dimensional visualization, and acquisition time of computerized image analysis for one-dimensional planar separation, known as computerized image analysis or video densitometry. This is an efficient, low-cost technique for quantitative and qualitative analysis of planar separations, e.g. planar chromatography and gel electrophoresis. The image of the TLC plate is captured in black and white, then the proper color channel of the image is selected in order to enhance the signal-to-noise ratio. To facilitate image evaluation a three-dimensional visualization of the planar image was applied by use of OpenGL technology. It was found that the sensitivity is increased by use of longer acquisition times whereas linearity of quantitative analysis is reduced.

Ind. J. Pharm. Sci. 70(5), 644-647 (2008). HPTLC of meloxicam on silica gel with ethyl acetate - cyclohexane - glacial acetic acid 325:175:1 with chamber saturation for 45 min. Quantitative determination by absorbance measurement at 353 nm. The method was linear in the range of 100-500 ng/zone, recovery was 100.3 %. The method was evaluated for stability (acid, base, thermal, oxidative, photodegradation) and degradation products were well separated from the main drug.

Ind. J. Pharma Sci. 70(6), 798-800 (2008). HPTLC of plumbagin in Drosera burmannii Vahl on silica gel with toluene - glacial acetic acid 55:1 (for alcoholic extracts) and toluene - chloroform - glacial acetic acid 10:10:1 (for aqueous extracts) with chamber saturation for 60 min. Evaluation in visible light at 425 nm. The alcoholic extract showed seven components, the main zone with hRf value of 56 corresponded to plumbagin. The aqueous extract showed two zones but no plumbagin.

J. Chinese Trad. Patent Med. 30 (11), 1635-1638 (2008). TLC of Sanjin Ganmao pill extracts on silica gel with 1) chloroform - methanol 17:3; 2) chloroform - methanol - formic acid 35:5:2; 3) chloroform - methanol - formic acid 90:10:1. Detection 1) by spraying with 10 % sulfuric acid in ethanol and heating; 2) by exposure to ammonia vapor. Identification by comparison with the standard hyperin and other standards of the component drugs.

by HPTLC. Abstract No. 9191, IHCB (2009). HPTLC of (-)hydroxy citric acid (the main constituent of Garcinia gummigutta fruits) on silica gel with n-propanol - water - glacial acetic acid 50:50:1. Quantitative determination by absorbance measurement at 210 nm. The hRf value was 46. The method was linear in the range of 100-1000 ng/spot, recovery was 99.8-100.9 %.

Abstract No. F-309, 61st IPC (2009). HPTLC of olopatadine HCl on silica gel with methanol - chloroform - 25 % ammonia 80:20:1. The hRf value was 46. Quantitative determination by absorbance measurement at 301 nm. The method was linear in the range of 100-900 ng/band.

Abstract No. F-246, 61st IPC (2009). HPTLC of levodopa and carbidopa on silica gel with acetone - chloroform - n-butanol - acetic acid - water 50:45:42:35:25. Quantitative determination by absorbance measurement at 283 nm. The method was linear in the range of 200-700 ng/band for both compounds, with a recovery of 98.7-99.9 %.

Ind. J. Pharma. Sci. 71(1), 95-97 (2009). HPTLC of emtricitabine and tenofovir on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 265 nm. The calibration curve was linear between 200 and 1000 ng with a regression coefficient of 0.9995.