J. Planar Chromatogr. 32, 25-30 (2019). HPTLC of asiaticoside (1) and madecassoside (2) in Centella asiatica on silica gel with chloroform - methanol - water 5:4:1. Quantitative determination by absorbance measurement at 202 nm. The hRF values for (1) and (2) were 72 and 47, respectively. Linearity was between 100 and 1500 ng/zone for both (1) and (2). The intermediate precision was below 4.3 % (n=3). The LOD and LOQ for (1) and (2) were 14 and 60 as well as 37 and 82 ng, respectively. Recovery rate was 103.3 % for (1) and 95.8 % for (2).

J. Planar Chromatogr. 32, 41-46 (2019). HPTLC of ayapanin in the leaves of Ayapana triplinervis on silica gel with ether - ethyl acetate 3:2. Quantitative determination by absorbance measurement at 254 nm. The hRF value of ayapanin was 56. Linearity was between 200 and 1000 ng/zone. The intermediate precision was below 0.8 % (n=6). The LOD and LOQ for ayapanin were 54 and 169 ng/zone, respectively. Recovery rate was 99.7 % for ayapanin.

J. Liq. Chromatogr. Relat. Technol. 37, 2784-2799 (2014). HPTLC of atorvastatin (1), ezetimibe (2) and fenofibrate (3) on silica gel with toluene – methanol – triethylamine 16:3:1. Quantitative determination by absorbance measurement at 245 nm. The hRF values for (1) to (3) were 10, 20 and 80, respectively. Linearity was in the range of 100-350 ng/zone for (1) and (2) and 1600-5600 ng/zone for (3). The intermediate/interday/intra-day precisions were below 1 % (n=3). The LOD and LOQ were 60 and 90 ng/zone for (1), 80 and 100 ng/zone for (2) and 90 and 150 ng/zone for (3), respectively. Recoveries were in the range of 97.6-102.6 %.

J. of China Pharm. 22 (17), 32-33 (2013). Guiqi Tongru Heji compound oral liquid is a herbal TCM preparation for the treatment of postpartum hypogalactia. For quality control, TLC on silica gel 1) for Vaccaria segetalis, with chloroform – methanol – water 15:7:2, detection by spraying with fresh solution of (bismuth subnitrate - glacial acetic acid – water 17:200:800) – 40 % potassium iodate – glacial acetic acid – water 1:1:4:15, evaluation in daylight; 2) for Scrophularia ningpoensis Hemsl., with ethyl acetate - methanol – formic acid – glacial acetic acid - water 30:1:2:2:4, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones were visible in daylight; 3) for Angelica sinensis, with n-hexane – ethyl acetate 4:1, detection under UV 366 nm.

J. Ethnopharmacol. 156, 309-315 (2014). HPTLC of (1) phyllanthin and (2) ellagic acid in the whole plant of Phyllanthus rheedei on silica gel with toluene - ethyl acetate - formic acid 6:2:1, detection at UV 366 nm. The hRF values of (1) and (2) were 47 and 27, respectively. Fractions rich in phyllanthin and ellagic acid showed anti-HBV and hepatoprotective activity.

lanceolata with an energy audit (thermal × microwave × acoustic):_x000D_ a case study of HPTLC determination with additional specificity using on-line/off-line_x000D_ coupling with DAD/NIR/ESI-MS. Phytochem. Anal. 25, 551-560 (2014). HPTLC of (1)taraxasterol, (2) taraxasterol acetate and (3) stigmasterol in P. lanceolata on silica gel with hexane - ethyl acetate 22:3, detection by spraying with anisaldehyde sulphuric acid reagent, followed by air drying for 40 min. Quantitative determination by absorbance measurement at 645 nm. The hRF values of (1) to (3) were 75, 33 and 19. Linearity was in the range of 1-5 μg/zone for (1) to (3). The intermediate intra-day and inter-day precisions were below 2.3 % (n=3). The LOD and LOQ were 530 and 1760 ng/zone for (1), 240 and 790 ng/zone for (2) and 110 and 370 ng/zone for (3), respectively. Recoveries were in the range of 98-105 % for (1), 101-131 % for (2) and 95-116 % for (3).

J. Liq. Chrom. Rel. Tech. 38, 521-531 (2015). HPTLC of pitavastatin calcium on silica gel with ethyl acetate – methanol – toluene – glacial acetic acid 40:10:50:1. Quantitative determination by absorbance measurement at 335 nm. The hRF value of pitavastatin was 40. Linearity was between 25 and 150 ng/zone. The LOD and LOQ were 5 and 14 ng/zone. The intermediate intra-day and inter-day precisions were below 2.9 % (n=3). Recoveries were found in the range of 100.2-102.4 %.

J. China Pharm. Univ. 22 (23), 33-35 (2013). Xiangshao Granules are a TCM preparation for the treatment of women’s breast distending pains. For quality control, TLC on silica gel (1) for Cyperus rotundus, with petroleum ether (60-90 °C) – ethyl acetate 15:1, detection at UV 254 nm; (2) for Atractylodes macrocephala Koidz. and atractylone with petroleum ether (60-90 °C) – ethyl acetate 50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4, followed by heating until zones are visible; (3) for Angelica dahurica (Fisch. ex Hoffm.) Benth. et Hook. f. ex Franch. et Sav. and imperatorin with petroleum ether (60-90 °C) – diethyl ether 1:1, detection at UV 366 nm; (4) for Epimedium brevicornum Maxim and icariin with ethyl acetate – butanone – formic acid – water 10:1:1:1, detection at UV 366 nm before and after spraying with 3 % aluminium trichloride in ethanol.