Marine Drugs 12 (5), 2633-2667 (2014). The saponin-enriched isobutanol-soluble fraction of a prepurified ethanol – water 7:3 extract of the viscera (all intern organs after removal of the body wall) of Holothuria lessoni Massin & al. (2009) was submitted to high performance centrifugal partition chromatography (HPCPC), before direct MS injection. For the monitoring of the HPCPC, TLC of subfractions on silica gel with the lower phase of chloroform – methanol – water 7:13:8. Detection under UV light and by spraying with 15 % sulfuric acid in ethanol followed by heating at 110 °C for 15 min, and evaluation in daylight, saponins are hued in maroon/dark purple. At least 39 new saponins and 36 reported triterpene glycosides (including holothurins, argusides and leucospilotasides), containing different aglycones and sugar moieties, were identified in the viscera of this sea cucumber.

J. AOAC Int. 98, 1226-1233 (2015). HPTLC of three phosphodiesterase type 5 inhibitors, sildenafil (1), vardenafil (2), and tadalafil (3), and eight of their analogs, hydroxyacetildenafil (4), homosildenafil (5), thiohomosildenafil (6), acetildenafil (7), acetaminotadalafil (8), propoxyphenyl (9), hydroxyhomosildenafil (10), hydroxyhomosildenafil (11) and hydroxythiohomosildenafil (12) in finished products on silica gel with TBME - methanol - ammonia 28% 20:2:1. Detection under 254 and 366 nm UV light and by absorbance measurement at 232 nm. LOD was 30 ng/zone for all substances. Confirmation by HPTLC-ESI-MS. The method was successfully_x000D_ applied to screening of 45 commercial lifestyle products. Of those, 31 products tested positive for at least one illegal component (sildenafil, tadalafil, propoxyphenyl, hydroxyhomosildenafil, or dimethylsildenafil).

J. Planar Chromatogr. 28, 343-351 (2015). TLC of cobalt(II) (1) and copper(II) (2) on silica gel impregnated with cetyltrimethylammonium bromide micelles in water. The mobile phase was acetone - 3 M hydrochloric acid 99:1 with 2.6 mM hexamethylenediamine. Detection by spraying with 1 % potassium ferrocyanide for (1) or 1% dimethylglyoxime solution in ethanol for (2). Quantitative determination by absorbance measurement at 470 and 615 nm, respectively. The hRF values for (1) and (2) were 65 and 26, respectively. The LOD was 0.02 ng/L for (1) and 0.1 µg/L for (2).

Hawksw & P. James. Quim. Nova. 39, 456-461 (2016). HPTLC of chloratranorin (1), atranorin (2), ursolic acid (3), 2'-O-methylphysodone (4), physodic acid (5), physodalic acid (6) and physodic acid (7) in Hypogymnia tavaresii on silica gel with toluene – acetic acid 17:3. Detection by spraying with sulfuric acid – acetic acid – water 1:20:4, followed by heating at 120 °C for 20 min. The hRF values of compunds (1) to (7) were 76, 74, 49, 33, 20, 19 and 18, respectively.

J. Liq. Chromatogr. Relat. Technol. 39, 312-321 (2016). HPTLC of lupeol in waxes of different cabbage varieties on RP-18 with ethyl acetate – acetonitrile 5:1. Detection by dipping into the anisaldehyde reagent (16 mL sulfuric acid were dropwise added to a cooled mixture of 20 mL glacial acetic acid and 170 mL methanol, then 1 mL of anisaldehyde was added), followed by heating at 110 °C for 30 s. Quantitative determination by absorbance measurement at 535 nm. The hRF of lupeol was 40. Linearity was in the range of 30-150 ng/zone. The LOD and LOQ were 7 and 20 ng/zone, respectively. Intermediate precisions were below 10 %. Recoveries were between 90.3 and 93.1 %.

Chinese J. of Inform. on TCM 20 (7), 59-61 (2013). Shengxinfa Jiaonang capsule is a herbal TCM preparation for treatment of hair loss caused by blood deficiency and kidney essence deficiency, scalp pruritus, alopecia areata, and postpartum alopecia. For quality control, TLC on silica gel (1) for Fallopia multiflora (Thunb.) Harald, with toluene – ethyl acetate – formic acid 15:2:1, detection in daylight; (2) for Psoralea corylifolia Linn., with n-hexane – ethyl acetate 4:1, detection by spraying with 10 KOH in methanol and viewing under UV 366 nm; (3) for Gastrodia elata Bl., with chloroform – ethyl acetate 1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible. Quantification of 2,3,5,4'-tetrahydroxy stilbene-2-Ο-β-D-glucoside by HPLC.

J. Sep. Sci. 39, 4258-4262 (2016). TLC of 35 model compounds on RP-2, RP-8, RP-18, alumina, cellulose, cyano, diol, amino phase, and silica gel with 20 pure solvents (acetone, acetonitrile, 1-butanol, 1-chlorobutane, chloroform, cyclohexane, 1,2-dichloroethane, dichloromethane, dioxane, ethanol, ethyl acetate, heptane, hexane, methanol, isooctane, 1-propanol, 2-propanol, t-butyl methyl ketone, tetrahydrofuran, and toluene). Parallel factor analysis was used to study the retention phenomena in quantitative manner.

Planta Medica 82 (16), 1395-1402 (2016). The hexane – ethyl acetate subfraction of the ethyl acetate fraction of an ethanolic Tabebuia roseoalba leaf percolation extract was subfractioned on a silica gel column with 42500 mL of a gradient of hexane, ethyl acetate, and methanol. For monitoring, TLC on silica gel with hexane – ethyl acetate 1:1, detection with anisaldehyde sulfuric acid reagent. Two fractions that gave only one visible zone on TLC, were identified by NMR as mixtures of stigmasterol and sitosterol, and of alpha- and beta-amyrins, respectively.