J. Liq. Chromatogr. Relat. Technol. 38, 1061-1067 (2015). HPTLC of ethambutol (1), isoniazid (2), pyrazinamide (3) and rifampicin (4) on silica gel with acetonitrile - chloroform - 2-propanol - 4N ammonia 34:31:34:1. Quantitative determination by absorbance measurement at 270 nm for (2) to (4) and 250 nm for (1) after a derivatization reaction. The hRF values for (1) to (4) were 9, 31, 48 and 73, respectively. Linearity was in the range of 300-1800 ng/zone for (1), 75-450 ng/zone for (2), 300-1200 ng/zone for (3) and 150-900 ng/zone for (4). LOD and LOQ were 94 and 286 ng/zone for (1), 20 and 62 ng/zone for (2), 18 and 55 ng/zone for (3) and 40 and 121 ng/zone for (4), respectively. The intermediate precision was below 2.5 % (n=6). Recoveries ranged between 97 and 101 %.

J. AOAC Int. 98, 857-861 (2015). _x000D_TLC direct bioautography of apigenin (1) and α-linolenic acid (2) in Matricaria recutita L. (chamomile) and Achillea millefolium L. (yarrow) tinctures on silica gel with chloroform - acetone 99:1. Bioautography by dipping into suspensions of Bacillus subtilis, S. aureus, S. epidermidis, Pseudomonas syringae pv. maculicola, Xanthomonas campestris pv., Escherichia coli and Aliivibrio fischeri for 10 s. In the case of soil bacteria and human pathogens, the plates were incubated at 37 °C for 5 h, followed by dipping into a solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 0.05 g/90 mL) for 5 s and further incubation at 37 °C for 2 h. The hRF values for (1) and (2) were 33 and 66, respectively.

with the reference of protostemonine. J. Planar Chromatogr. 28, 448-451 (2015). HPTLC of protostemonine in Stemona sessilifolia on silica gel with ammonium hydroxide solution (25 %, v/v) – acetone – ethyl acetate – cyclohexane 1:4:4:6. Detection by spraying with diluted bismuth potassium iodide solution followed by drying at room temperature and spraying with 5 % sodium nitrite in 70 % ethanol solution. The hRF value for protostemonine was 30. The intermediate precision was below 2 % (n=5).

Phytochem. Anal. 26, 404-412 (2015). TLC-autography of physostigmine in an extract of Brassica rapa with the acetylcholinesterase inhibitor on a TLC plate sprayed with a solution of α-naphthyl acetate (2.5 mg/mL) – fast blue B (2.5 mg/mL) 4:1 and with a acetylcholinesterase agar solution distributed over the TLC plate. The plate was developed with dichloromethane - methanol 9:1, ethyl acetate - methanol - water 5:5:2 and toluene - chloroform - ethanol 57:114:29. Further extraction and analysis with high resolution mass spectrometry. _x000D_

J. Planar Chromatogr. 29, 108-112 (2016). HPTLC of water-soluble vitamins (B1, B2, B6, C, and B12), amino acids and β-blocker enantiomers (carvedilol, (S)-(−)-propranolol hydrochloride, (±)-propranolol hydrochloride, and (±)-sotalol hydrochloride) on novel core–shell polymers consisting of hyperbranched (poly(ethyleneimine)) core surrounded by oligosaccharide shell (PEI-OS) as a component of the silica gel phase. The influence of the weight of the hyperbranched PEI core (5 and 25 kDa) was investigated. β-blocker enantiomers were separated using acetonitrile - methanol 7:3. PEI-maltose and PEI-lactose were the most effective polymers for the chiral separation of β-blockers.

Planta Medica 82, 09/10, 903-909 (2016). To monitor the sorbent phase purification of a chloroform subfraction of the sponge Myxilla incrustans, TLC of fractions on silica gel with dichloromethane – methanol – formic acid 900:100:1, detection with Dragendorff’s reagent. The four positive fractions were purified by preparative HPLC, yielding three new N-acyl dopamine/dehydrotyrosine glycosides: myxillins A, B and C._x000D_

J. Planar Chromatogr. 29, 229-231 (2016). HPTLC of imipenem, meropenem, doripenem and eratapenem on silica gel G with 11 developing systems. Detection by spraying with iodine solution (0.2 g of potassium iodine and 0.4 g of iodine_x000D_ mixed in 20 mL of ethanol and 5 mL of hydrochloric acid), ninhydrin reagent (0.1 % ninhydrin in ethanol) or potassium permanganate solution (1 mg dissolved in 10 mL of water). The hRF values for the studied carbapenems in each developing system ranged between 13 and 58.

Chinese J. Hosp. Pharm. 35 (11), 992-996 (2015). Sisheng Keli granule is a herbal TCM preparation for treating hematemesis, hematuria, allergic purpura and thrombocytopenic purpura, etc. For quality control, TLC on silica gel (1) for Artemisia argyi Levl. et Vant., with petroleum ether (60-90 °C) – toluene – acetone 20:16:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible; (2) for Platycladus orientalis (L.) Franco, with toluene – ethyl acetate – formic acid 5:2:1, detection by spraying with 1 % aluminium trichloride in ethanol and detection under UV 366 nm; (3) for Lotus leaf, with chloroform – acetone – 10 % ammonia in ethanol 60:20:1, detection by spraying with 5 % potassium bismuthate solution and heating at 105 °C until the zones are visible. Quantification of nuciferin and verbascoside by HPLC.