J. Liq. Chromatogr. Relat. Technol. 39, 277-280 (2016). HPTLC of azithromycin (1), imipramine HCl (2), and sulfadoxine + pyrimethamine (3/4) on silica gel with methanol – ethyl acetate – concentrated ammonia 40:10:1 for (1), methanol – ammonia 96:1 for (2) and ethyl acetate – methanol 5:1 for (3/4). Quantitative determination by absorbance measurement at 254 nm. The hRF values were 54 for (1), 40 for (2), 67 for (3) and 45 for (4). Precisions for recovery were within 5 % and RSDs for triplicate assays and validation analyses at 50, 100, and 150 % spike levels were within 3 %._x000D_

Chinese J. Mod. Drug Appl. 8 (5), 3-4 (2014). Danshen Wuweizi Pian tablet is a herbal TCM preparation for the treatment of coronary heart disease, acute and chronic persistent hepatitis and neurasthenia. For quality control, TLC (1) for Salvia miltiorrhiza Bge. and the standard tanshinone IIA, on silica gel with toluene – ethyl acetate 19:1, detection in daylight; (2) for Schisandra chinensis (Turcz.) Baill., on silica gel with petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 254 nm.

J. Planar Chromatogr. 29, 336-340 (2016). HPTLC of isoalantolactone (1), germacranolide (2), β-sitosterol (3), and lupeol (4) in the roots of Inula cappa on silica gel with toluene ‒ methanol 47:3. Detection by dipping into anisaldehyde reagent followed by heating at 105 ºC until colored bands appeared. Quantitative determination by absorbance measurement at 525 nm. Linearity ranged from 10-70 μg/mL for (1), 10-60 μg/mL for (2), 8-48 μg/mL for (3) and 15-90 μg/mL for (4). The intermediate precisions were below 0.9 % (n=7). The LODs and LOQs were 5 and 10 μg/mL for (1), (2) and (4) and 2 and 6 μg/mL for (3). Average recoveries were between 98.3 and 99.0 % for (1) to (4).

Microbiol. Res. 186-187, 119-131 (2016). TLC bioautographic analysis of active extracts of the producing strain Natrinema gari QI1 on silica gel with n-butanol – acetic acid – water 3:1:1. The agar media containing the indicator strain Haloferax mediterranei SAG3 was applied directly onto the developed TLC plate and incubated at 40 °C for 7 days. The compound with the most significant inhibition of Haloferax mediterranei was at hRF 29.

Chinese J. of Drug Evaluation 32 (4), 193-194 (2015). Fortune Paulownia leaf, the dry leaf of Paulownia fortunei (Seem.) Hemsl, is a TCM raw material used for treatment of carbuncles, furuncles, trauma, hemorrhage, etc. For quality control, TLC of its extracts on silica gel previously impregnated with 1 % iodine in dichloromethane and developed with cyclohexane – ethyl acetate – glacial acetic acid 20:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones are clearly visualized, viewing (A) in white light and (B) under UV 366 nm. Identification by fingerprint comparison with the standards oleanolic acid (hRf 67) and ursolic acid (hRf 60) and the reference raw material in parallel.

J. Ethnopharmacol. 198, 158-166 (2017). HPTLC of chlorogenic acid in the stems of Solanum xanthocarpum on silica gel with ethyl acetate – glacial acetic acid – formic acid – water 8:3:3:4. Quantitative determination by absorbance measurement at 254 nm. The hRF value for chlorogenic acid was 56.

J. Liq. Chromatogr. Relat. Technol. 40, 297-303 (2017). HPTLC of amygdalin on RP-18 with acetonitrile – water 1:1. Quantitative determination by absorbance measurement at 210 nm. The hRF value for amygdalin was 60. Linearity was between 2.5 and 50 μg/zone. LOD and LOQ were 1 and 4 μg/zone, respectively. The intermediate precision was <2 % (n=3). Recovery rate ranged from 99.9 to 100.6 %.

J. Chromatogr. Sci. 54 (8), 1421-1427 (2016). Development of a simple, cost-effective and reliable method for quantification of naphthoquinones, found as natural red color pigments in roots of Arnebia benthamii (Wall. ex G.Don) I.M. Johnst., by HPTLC on silica gel with hexane – ethyl acetate – methanol 16:3:1. Determination of shikonin (hRf 37) and β,β-dimethylacryl shikonin (hRf 58) in the methanol extract by densitometry at 520 nm. The linearity was 0.1-8 µg/zone. The average recovery was >97 % for both and the intraday and interday precision for the quantification of naphthoquinones was good (%RSD <2.0 %). The LOD were 13 and 15 ng/zone for shikonin and β,β-dimethylacryl shikon and the LOQ were 39 and 44 ng/zone, respectively.