Ind. J. Pharm. Sci. 69 (6) 841 - 844 (2007). HPTLC of artesunate on silica gel with toluene - ethyl acetate - acetic acid 20:80:2. Detection by treatment with vanillin reagent (1 % vanillin in 5 % ethanolic sulphuric acid) leads to pink zones which are stable for more than a day. Quantitative determination by absorbance measurement at 520 nm. The hRf value for artesunate was 44. Linearity was between 100 and 600 ng per spot. Recovery (by standard addition method) was 98.9 - 99.9 % for tablets and injections.

Biochem. Biophys. Res. Commun. 378, 224-229 (2009). TLC of the hydrolytic action patterns of the purified Nostoc punctiforme debranching enzyme on the following substrates: pullulan, amylopectin, soluble starch, amylose, cyclodextrins, and maltooligosaccharides (form glucose to maltoheptaose), on silica gel with 1-propyl alcohol – ethyl acetate – water 6:2:3. Detection by dipping into 0.3 % N-(1-naphthyl)-ethylenediamine and 5 % sulfuric acid in methanol, followed by heating for 10 min at 110 °C. Quantitative determination by radioactivity measurement of the 14C-labeled maltooligosaccharides.

Ind. J. Pharm. Sci. 70(3), 398 - 400 (2008). HPTLC of ibuprofen and pseudoephedrine HCl on silica gel with tert-butanol - ethyl acetate - acetic acid - water 7:4:2:2. Quantitative determination by absorbance measurement at 254 nm. The hRf value of pseudoephedrine was 68 and of ibuprofen 91. The method was linear in the concentration range of 45.6 - 75.6 µg/mL for ibuprofen and 6.8 - 11.3 µg/mL for pseudoephedrine. The recovery was between 100.7 and 101.0 % for both compounds. The method was suitable for routine quality control.

Asian J. Chem. 19(7), 5582-5586 (2007). HPTLC of telmisartan and hydrochlorothiazide on silica gel with ethyl acetate - chloroform - methanol 10:3:1. Absorbance measurement at 270 nm. The method was linear in the range of 500-750 ng/µL and 1600-2400 µg/µL for hydrochlorothiazide and telmisartan respectively. The recovery was 99.4-99.6 % for both compounds.

Ind. J. Pharm. Sci. 70(2), 251-255 (2008). HPTLC of olanzapine and fluoxetine on silica gel with methanol - toluene 2:1. Quantitative determination by absorbance measurement at 233 nm. The method was linear in the range of 100-800 ng/spot for olanzapine and 1000-1200 ng/spot for fluoxetine. Recovery was 99.4-100.4 % for both compounds. Forced degradation studies (acid, base, oxidation, photolyses and thermal) revealed that all the degradation products were well resolved from the principal compound. The method was suitable for routine quality control.

Biochem. Biophys. Res. Commun. 378, 772-776 (2009). TLC of lipids from adipocytes on silica gel pre-treated with a solution containing methanol – water – potassium oxide – EDTA 60:40:1%:1mM and activated at 100 °C for 1 hour. The plate was developed with n-propanol – water – glacial acetic acid 65:34:1. Detection and quantitative determination by autoradiography using storage phosphor technology.

Abstract No. 9147, IHCB (2009). HPTLC of quercetin in methanolic leaf extracts of Viola odorata on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. Quantitative determination by absorbance measurement at 366/>400 nm for quantification. The extract contained 0.36 % quercetin.

Ind. J. of Pharma Sci. 70(6), 847-851 (2008). HPTLC of apigenin in segregated parts (leaves, stems, flowers and fruits) of Turnera aphrodisiaca on silica gel with toluene - ethyl acetate 1:4. Quantitative determination by absorbance measurement at 336 nm. Flowers were found to contain maximum amount of apigenin whereas leaves contained the least. Apigenin contents in methanolic extracts of aerial plant parts were fourteen times lower than in acid hydrolyzed methanolic extracts, indicating the presence of most of apigenin in glycosidic form. The plant material collected in September showed maximum contents of apigenin.