Pharmacogn. Mag. 19, 917-925 (2023). HPTLC profiling of Guggulutiktam Kashayam on silica gel with toluene - ethyl acetate - methanol 7:3:1. Detection by spraying with anisaldehyde sulphuric acid reagent. Qualitative identification under UV light at 254 and 366 nm. 

J. Ethnopharmacol. 321, 117566 (2024). HPTLC of Evolvulus alsinoides on silica gel with toluene - ethyl acetate - formic acid 6:3:1. Qualitative identification under UV light between 450 and 550 nm. The hRF values of methanol extract ranged from 3 to 64. 

Phytochem. Anal. 35, 10.1002/pca.3297 (2023). HPTLC of aerial parts of Hypericum perforatum on silica gel with ethyl acetate - toluene - acetic acid - formic acid 7:3:1:1. Detection by spraying with 50 % sulfuric acid and vanillin, followed by heating and visualization under UV light at 254 and 366 nm. 

Chinese J. Veter. Drug 57 (6), 52-57 (2023). Wupi Koufuye is a multi-ingredient TCM veterinary drug clinically used for the prophylaxis and treatment of gout in animals. For quality control, TLC on silica gel A) for White Mulberry root bark, developed twice with cyclohexane – ethyl acetate – methanol – concentrated ammonia solution 75:75:15:2, detection at UV 366 nm; B) for Pericarpium citri reticulatae, developed with trichloromethane - methanol – concentrated ammonia solution 100:30:3, detection by spraying with 0.5 % ninhydrin in ethanol and heating at 105 °C until the zones are visible in daylight; C) for Zingiber officinale Rosc., with n-hexane, detection by spraying with 0.5 % vanilin in sulfuric acid – ethanol 4:1 followed by heating at 105°C until the zones are visible in daylight.

J. China Pharm. 32 (6), 71-74 (2023). Shegan Kangbingdu Koufuye is a multi-ingredient TCM oral liquid with antiviral effects. For quality control, TLC on silica gel A) for Blackberrylily rhizome, with chloroform – methanol – glacial acetic acid 200:30:2, detection by spraying with 2 % FeCl3 in ethanol, evaluation in fluorescence; B) Radix Bupleuri,  with chloroform – methanol – water 13:7:2, detection by spraying with 2 % 4-dimethylaminobenzaldehyde in 40 % sulfuric acid solution and heating at 60 °C until the zones are visible at UV 366 nm; C) for Folium Isatidis and Radix Isatidis, with cyclohexane - chloroform – propanone 5:4:2, detection in fluorescence light; D) for Capillary Wormwood Herb, Honeysuckle and Dandelion, with toluene – ethyl formate – formic acid 6:4:1, detection in fluorescence light.

PLoS ONE 18(11), e0294775 (2023). Sample was an Azadirachta indica seed extract (Meliaceae). TLC on silica gel with different mobile phases: (1) diethyl ether – methanol 49:1; (2) diethyl ether – acetone 2:1; (3) isopropanol – n-hexane 11:9; (4) dichloromethane – methanol – acetic acid 95:5:1. After 30 min hot air drying,detection under UV light. The hRF values of azadirachtin A (a limonoid) were 75, 42, 44, 55, respectively. Mobile phase (1) was therefore chosen as solvent for purification of azadirachtin A and for its quantification by Fourier transform infrared spectroscopy (FTIR).

Chinese J. Pharm. Research 42 (2), 98-104 (2023). Ganoderma lucidum is the dry fruiting body of fungus, a medicinal plant, having the effect of relieving asthma and relieving nerves. Ganoderma lucidum is widely distributed in China and mainly concentrated in Zhejiang, Anhui, Shandong, Jilin, and Fujian. In this study, the HPTLC method for quality detection and origin traceability was established towards the medicinal material produced in Fujian and other areas. HPTLC of the ethanol extracts of the sample materials on silica gel with chloroform – acetonitrile – methanol – formic acid 130:15:2:2, three times to distances of 3 cm, 6 cm, 8 cm. Detection by spraying with 3 % sulfuric acid in ethyl acetate and heating at 105°C until the bands are clearly visualized, evaluation at UV 366 nm. The bands on the thin layer chromatogram were identified by fingerprint comparison using standard samples, and cluster analysis for the data of samples from different producing areas was performed using digital characterization. As results, the HPTLC analysis detected 17 distinct bands and among them, 11 components were identified, e.g. ganoderenic acid C (hRf 0.31), ganoderic acid C2 (hRf 33), ganoderic acid l (hRf 35), ganoderic acid G (hRf 41), ganoderic acid A (hRf 44), ganoderic acid B (hRf 46), ganolactone B (hRf 53), 3β,7β,15β-trihydroxy-11,15-dihydroxy-lanostane-8-alkene-24→20 lactone (hRf 56), ganoderenic acid D (hRf 61), ganoderic acid D (hRf 63) and 20 (21)-dehydrolucidenic acid A (hRf 65). A total of 10 HPTLC bands were identified in Fujian Ganoderma lucidum, among which ganoderic acid C2, ganoderic acid D and two unknown component bands (hRf 14 and 17) were unique and could be used as markers of Fujian Ganoderma lucidum. Thus samples of Fujian Ganoderma lucidum can be differentiated from those of other areas, when the Euclidean distance is 25 in Euclidean cluster analysis.

J Chromatogr Sci, 61 (3), 269-278 (2023). Development of a method for identification, quantification and stability study of linalool in Homalomena aromatica Schott, a herb with anti-rheumatism, nourishing and strengthening effects, and for the treatment of stomach disease. HPTLC hyphenated with MS for linalool on silica gel with toluene - ethyl acetate 19:1. Detection  with p-anisaldehyde reagent. The hRf of linalool is 35, identification of linalool at m/z 137, (M + H)+ by using MS technique. Study of the stability of linalool by investigating the effect of acid, base, UV, sunlight, thermal stress and H2O2 on it, the highest effect was observed with acidic pH with a 65 % degradation. The content of linalool was 58 % in the volatile oil of H. aromatica.