J. Pharm. Biomed. Anal. 47, 790-794 (2008). HPTLC of the steviolbioside, stevioside and rebaudioside A in Stevia rebaudiana leaves on silica gel with ethyl acetate - ethanol - water 20:5:3. Detection by spraying with acetic anyhdride - sulphuric acid - ethanol 1:1:10 reagent. Quantitative determination by absorbance measurement at 510 nm. Linearity was in the range of 160-960 ng/spot for steviolbioside, 1-6 µg/spot for stevioside and 0.5-3 µg/spot for rebaudioside A with good correlation coefficients (0.998-0.999). The method was used for the assay of steviol glycosides in S. rebaudiana leaves collected from ten different locations.

60th Indian Pharmaceutical Congress PG-263 (2008). HPTLC of rutin in methanolic extracts of fresh leaves of Moringa pterygosperma on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. Quantitative determination by absorbance measurement at 254 nm. The method provided good resolution of rutin from other constituents of the plant.

J. Sep. Sci. 32, 3239-3245 (2009). HPTLC of shikonin (1), acetylshikonin (2), and beta-acetoxyisovalerylshikonin (3) in four species of Arnebia on RP-18 with acetonitrile - methanol - 5 % formic acid 20:1:4. Quantitative determination by absorbance measurement at 520 nm. Linearity was in the range of 100-600 ng/zone for (1) and (2) and 100-1800 ng/zone for (3). The limits of detection for (1), (2) and (3) were 18, 15 and 12 ng/zone, respectively, while the limits of quantification were 60, 45 and 40 ng/zone, respectively.

Learned J. Dali Acad. (General Issue) 8 (10), 3-6 (2009). TLC of puerarin (in extracts obtained from different parts of Radix Puerariae) on silica gel with trichloromethane – methanol – water 14:5:1. Detection under UV 254 nm. The maximum amount of puerarin was found in the bine of the drug, followed by that in the root, whereas no puerarin was found in the flower and fruit.

International Seminar on Herbal Drug Research, PN-013 (2009). HPTLC of ethyl acetate extracts of seeds of Eugenia jambolana on silica gel with chloroform - acetone - formic acid 150:33:17. The antioxidant activity of different extract was assessed by DPPH method. The proposed method was applied to plant extract as well as to polyherbal formulation. The ethyl acetate extract was found to have good antioxidant activity. For HPLC fingerprint profiling a RP18 column was used with 80 % methanol as mobile phase.

Abstract No. C-293, 61st IPC (2009). HPTLC of gallic acid in fruits of Phyllanthus emblica on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:4:1. The hRf value of gallic acid was 40. Quantitative absorbance measurement at 280 nm. The method was linear in the range of 40-240 ng/band. The method was reproducible and suitable for quality control.

Ind. J. Pharma. Sci. 72(1), 39-45 (2010). The Ayurvedic Pharmacopoeia of India recognized roots of the three plants Cissampelos pareira, Cyclea peltata, and Stephania japonica (all Menispermaceae) as source for the marketed drug Patha. HPTLC fingerprint analysis of methanolic extracts of roots of all three plants on silica gel with n-butanol - ethyl acetate - formic acid - water 3:5:1:1. Detection under UV 365 nm (crude extract) and 295 nm (total alkaloids). Quantification of the marker berberine by HPLC. The three plants exhibit significantly different physico-chemical features and chromatographic fingerprints. Roots of C. pareira contained the highest concentration of berberine, S. Japonica contained very low amounts and C. peltata no berberine at all.

Modern J. of Integrated Trad. Chinese & Western Med. 19(31), 3439-3441 (2010). TLC on silica gel with chloroform – methanol – water 13:7:2. Detection by spraying with 10 % slfuric acid in ethanol and heating at 105 °C until the zones are visualized, evaluation under UV 366 nm. Identification of the component drugs Radix Astragali and Rhizoma Chuanxiong P.E by comparison of the retention values and color of the zones by the active compounds astragaloside and ferulic acid in the individual drug.