Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      128 093
      High-throughput enzyme inhibition screening of 44 Iranian medicinal plants via piezoelectric spraying of planar cholinesterase assays
      E. AZADNIYA, I. THOMÄ, J. BAAKE, Gertrud E. MORLOCK* (*Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Giessen, Germany; gertrud.morlock@uni-giessen.de)

      Journal of Chromatography B, 1184, 122956 (2021). Test for acetyl- and butyrylcholinesterase (AChE and BChE) inhibition without development of piperin (standard inhibitor of AChE and BChE) and ethanol – water (3:2) extracts of Iranian plants, on HPTLC silica gel prewashed twice with methanol – water 3:2 and dried 60 min at 120°C. After sample application the plate was immersed (speed 3.5 cm/s, time 2 s) into enzyme solution (6.6 units/mL AChE or 3.3 units/mL BChE in TRIS buffer 0.05 M, with bovine serum albumin 0.1 %, pH 7.8), incubation 25 min at 37°C and immersion (speed 3.5 cm/s, time 1 s) into chromogenic substrate solution (α-naphthyl acetate 0.1 % and Fast Blue salt B 0.2 % in ethanol – water, 1:2). Seven mobile phases were tested for the active samples. Best separation was obtained with toluene – ethyl acetate – formic acid – water 4:16:3:2 and with toluene – ethyl acetate – methanol 6:3:1. Before enzymatic assay, plates developed with acidic mobile phases were neutralized by spraying 3 mL citrate phosphate buffer (Na2HPO4 8 %, citric acid q.s. ad pH 7.5) followed by 10 min of automatic drying. Enzymatic assay was performed using a piezoelectric spraying device: a) pre-wetting by spraying 1 mL TRIS buffer (0.05 M, pH 7.8); b) spraying 3 mL of the enzyme solution; c) incubation 25 min in a humid box at 37°C; d) spraying 0.5 mL substrate solution; e) 5 min drying at room temperature, and then 10 min of automatic drying. By spraying, zone shift and zone diffusion, which occurred with plate immersion, were avoided. For development control, derivatization was done by piezoelectrically spraying 4 mL of sulfuric anisaldehyde reagent (anisaldehyde – sulfuric acid – acetic acid – methanol, 1:10:20:170), followed by heating 3 min at 110°C. For identification of zones of interest, direct elution with methanol from underivatized HPTLC plates through a TLC-MS interface directly to a MS. Identified zones were 3-O-acetyl-β-boswellic acid (triterpenoid) from Boswellia carteri gum-resin (Burseraceae), pimpinellin and psoralen (furocoumarins) from Heracleum persicum flowers (Apiaceae), oleuropein (seco-iridoid) from Olea europaea leaves (Oleaceae), harmine, harmaline, vasicine, deoxyvasine (alkaloids) from Peganum harmala seeds (Zygophyllaceae), costic acid (sesquiterpene) from Nardostachys jatamansi hypocotyl (Valerianaceae), elaidic, linoleic, palmitic, palmitoleic acids (fatty acids) from Pistacia atlantica fruits (Anacardiaceae).

      Classification: 4e, 8b, 11a, 15a, 22, 32e
      128 089
      Effect-directed profiling of 32 vanilla products, characterization of multi-potent compounds and quantification of vanillin and ethylvanillin
      Gertrud E. MORLOCK*, M. BUSSO, S. TOMEBA, A. SIGHICELLI (*Institute of Nutritional Science, and TransMIT Center for Effect-Directed Analysis, Justus Liebig University Giessen, Giessen, Germany; gertrud.morlock@uni-giessen.de)

      J Chromatogr. A, 1652, 462377 (2021). Samples were vanilla tinctures, water − ethanol − ethyl acetate 1:1:1 extracts of vanilla-flavored food products and of natural Vanilla sp. (Orchidaceae) pods, oleoresin, paste and powders, as well as calibration standards of vanillin (1) and ethylvanillin (2). HPTLC on silica gel with n-hexane – ethyl acetate 1:1 for profiling, 3:2 for quantification. Other mobile phases were also tested and given in the supplement. Compounds (1) and (2) (hRF 68 and 82, respectively) were quantified by absorbance densitometry (at maximal wavelength 310 nm, deuterium lamp, scanning speed 10mm/s). Contents were found to be between 1 μg/g and 36 mg/g for (1) and null for (2) except in one tincture (62 µg/mL). Derivatizations performed for five assays: A) to detect radical scavengers, immersion (speed 3 cm/s, time 5 s) into DPPH• (0.5 mM in methanol), followed by drying for 90 s at room temperature and 30 s at 60 °C; B) to detect activity against Gram-negative bacteria, immersion (speed 2 cm/s, time 3 s) into Aliivibrio fischeri suspension, followed by recording the bioluminescence; C) to detect activity against Gram-positive bacteria, immersion (speed 3.5 cm/s, time 6 s) into Bacillus subtilis, followed by incubation 2 h at 37 °C, immersion in MTT solution, incubation for 30 min at 37 °C and heating for 5 min at 50 °C; D) to detect acetylcholinesterase (AChE) inhibitors, immersion (speed 2.5 cm/s, time 2 s) into AChE solution (666 units in TRIS buffer 0.05M, with bovine serum albumin 0.1 %, pH 7.8), incubation for 25 min at 37 °C and immersion into substrate solution (α-naphthyl acetate 0.1 % and Fast Blue salt B 0.18 % in ethanol – water, 1:2; E) to detect tyrosinase inhibitors, spraying with enzyme solution (400 unit/mL, in phosphate buffer 0.02 M, pH 6.8), followed by 2 min drying, immersion into substrate levodopa (18 mM in phosphate buffer, pH 6.8), 10 min incubation at room temperature and drying. For identification, zones of interest were transferred with methanol from underivatized HPTLC layer through a TLC-MS interface and a filter frit directly to a Quadrupole-Orbitrap MS (heated electrospray ionization, probe heater at 270°C, spray voltage 3.5kV, lock masses acetic acid for negative, dibutyl phthalate for positive ionization, mode full HR-MS scan in m/z range 50–750). Afterwards, the following substances assigned by MS were confirmed by using HPTLC comparison with standards: (1) and (2), vanillyl alcohol, vanillic acid, ethyl vanillyl ether, coumarin, 4-hydroxybenzoic acid, 4-methoxybenzoic acid, 4-hydroxybenzaldehyde, 4-allyl benzoic acid, oleamide, triacetin.

      Classification: 4e, 7, 8b, 32e
      128 034
      Quantification of gymnemagenin and β-sitosterol in marketed herbal formulation by validated normal phase HPTLC method
      S.E. POTAWALE, S.Y. GABHE*, K.R. MAHADIK (*Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Pune, India; satish3619@rediffmail.com)

      Chromatography Research International 2014, 626801 (2014). HPTLC of extracts of Gymnema sylvestre (Apocynaceae) in tablets, as well as standards for calibration, on silica gel (prewashed with methanol and activated at 120°C for 15 min) with toluene – ethyl acetate – methanol 65:25:14. Derivatization by immersing into sulfuric acid (5 % in methanol) and heating at 110°C for 4 min. Densitometric evaluation within 25 min in absorbance mode at 423 nm, which was the optimal wavelength for quantifying simultaneously the triterpenoid gymnemagenin (hRF 27, linearity range 100–1200 ng/band, LOD 32 ng/band, LOQ 53 ng/band) and β-sitosterol (hRF 78, linearity range 200–1200 ng/band, LOD 97 ng/band, LOQ 159 ng/band). Interday and intra-day precisions as well as recovery rates provided relative deviation values below 1 %. This method was used to determine the analyte contents in the tablets (0.041 % gymnemagenin and 0.138 % β-sitosterol), as well as to confirm the stability of the analytes in solution at room temperature after 48h.   

      Classification: 15, 32e
      128 035
      The bacterial microbiome of the long-term aquarium cultured high microbial abundance sponge Haliclona cnidata – sustained bioactivity despite community shifts under detrimental conditions
      J. SCHELLENBERG, J. REICHERT, M. HARDT, I. KLINGELHÖFER, G. MORLOCK, P. SCHUBERT, M. BIŽIĆ, H.-P. GROSSART, P. KÄMPFER, T. WILKE, Stefanie P. GLAESER* (*Research Centre for BioSystems, Land Use and Nutrition, Institute of Applied Microbiology, Justus Liebig University Giessen, Giessen, Germany; stefanie.glaeser@umwelt.uni-giessen.de)

      Frontiers in Marine Science 7, 266 (2020). Methanol extracts from marine sponge Haliclona cnidata (Chalinidae) submitted to different stresses (antibiotics and/or darkness) were separated on HPTLC silica gel with an automated 15-step gradient based on methanol, dichloromethane and n-hexane. Bioluminescence was recorded after immersing the HPTLC plates into Aliivibrio fischeri suspension. Antibacterial activity and quorum sensing enhancement were analysed on software, and Pearson’s similarity coefficient was applied to generate similarity matrices for cluster analysis (UPGMA, Unweighted Pair Group Method with Arithmetic Mean). Only slight differences were observed, especially in QS enhanced zones in stressed vs. control cultures.

       

      Classification: 32e
      128 059
      Bioautography and liquid chromatography‒mass spectrometry studies of Meyna spinosa Roxb. ex Link leaf methanolic extracts
      S. KADIRVELU, S. DAMLE, A. PILLAI (*National Facility for Biopharmaceuticals, G.N. Khalsa College, Matunga, Mumbai 400019, India, sagarika.damle@kccollege.edu.in)

      J. Planar Chromatogr. 34, 403-410 (2021). HPTLC of Meyna spinosa on silica gel with ethyl acetate - water 10:1. Detection by spraying with anisaldehyde‒sulfuric acid reagent and visualized under UV light at 366 nm. Further analysis by high-performance liquid chromatography coupled with electrospray ionization‒quadrupole-time of flight‒mass spectrometry (LC‒ESI‒Q-TOF‒MS).

      Classification: 32e
      128 060
      A validated high‑performance thin‑layer chromatography method for the determination of two bioactive lignans, phyllanthin and hypophyllanthin, in the seasonal variation study of Phyllanthus amarus
      S. KHATOON*, S. IRSHAD (*Pharmacognosy Division, CSIR-National Botanical Research Institute, Post Box No. 436, Rana Pratap Marg, Lucknow 226001, India, sayyadak@gmail.com)

      J. Planar Chromatogr. 34, 427-435 (2021). HPTLC of phyllanthin (1) and hypophyllanthin (2) in Phyllanthus amarus on silica gel with toluene - ethyl acetate 17:3. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 21 and 35, respectively. Linearity was between 2 ans 7 μg/zone for (1) and (2). LOD and LOQ were 1 and 3 μg/zone for (1) and (2), respectively. Intermediate precisions were below 2 % (n=3).

      Classification: 7
      128 061
      Comparative qualitative analysis of different classes of compounds in selected Australian and Indian Eucalyptus and Corymbia species: a convenient de‑replication method for the eucalypts
      I. SARAF, K. MARSH, V. KUMAR, W. FOLEY, I. SINGH* (*Department of Natural Products, National Institute of Pharmaceutical Education and Research (NIPER), Sector‑67, Sahibzada Ajit Singh Nagar, Punjab 160062, India, ipsingh67@yahoo.com)

      J. Planar Chromatogr. 34, 377-401 (2021). HPTLC of flavonoid glycosides, phloroglucinol glycosides, monoterpene glycosides and monoterpene sugar esters, triterpenoids, phloroglucinols, monomeric phloroglucinols, dimeric phloroglucinols and phloroglucinol-terpene adducts in 15 eucalypts (13 Eucalyptus and 2 Corymbia) on silica gel with multiple mobile phases. Detection by spraying with anisaldehyde-sulfuric acid reagent. Zones were scanned from 200 to 700 nm.

      Classification: 8a, 15a
      128 062
      Quantitative phytochemical and chromatographic analysis of phenolic compounds in methanolic leaf extract of Costus pictus D. Don
      G. DEVI (Department of Botany, Bangalore University, Bangalore, Karnataka, India, radha.kle@gmail.com)

      J. Planar Chromatogr. 34, 437-446 (2021). HPTLC of phenolic compounds in the leaf extract of Costus pictus on silica gel with chloroform - glacial acetic acid - methanol - water 16:8:3:2. Compounds with the same hRF values were analyzed by high-performance liquid chromatography, diode array detector and tandem mass spectrometry.

      Classification: 7
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