J. China Pharm. 25 (2), 72-74 (2016). Baibai Keli granule is a herbal TCM for treatment of menorrhagia caused by Qi deficiency and blood-heat. For quality control, TLC of its extracts on silica gel (1) for Codonopsis pilosula (Franch.) Nannf., with n-butanol – formic acid 1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visualized in daylight; (2) for Poria Cocos (Schw.) Wolf dry sclerotium, with petroleum ether (30-60 °C) – acetone 4:1, detection under UV 366 nm; (3) for Atractylodes macrocephala, with petroleum ether (60-90 ºC) – ethyl acetate 20:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 4:1 and heating at 105 °C until the zones are visualized in daylight; (4) for Astragalus membranaceus (Fisch.) Bunge., with the lower phase of chloroform – ethyl acetate – methanol – water 3:3:2:1 under 10 ºC overnight, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones are visualized, evaluation (A) in daylight and (B) at UV 366 nm. Quantification of glycyrrhizic acid by HPLC.

J. Planar Chromatogr. 30, 495-501 (2017). HPTLC of stigmasterol in Lasiurus scindicus on silica gel with toluene ‒ methanol ‒ formic acid 90:10:3. Detection by spraying with anisaldehyde ‒ sulfuric acid reagent, followed by heating at 150 °C for 5 min. Quantitative determination by absorbance measurement at 530 nm. The hRF value for stigmasterol was 27. Linearity was between 2 and 12 ng/zone. LOD and LOQ were 0.07 and 0.2 ng/zone. Average recovery was 99.3 %.

Planta Med. 83, 1097-1102 (2017). The fractionations on a silica gel column (with a gradient of ethyl acetate, methanol and petroleum ether/dichloromethane) of the dichloromethane and methanol extracts of Antidesma ghaesembilla bark were monitored by TLC on silica gel with chloroform – methanol – water – formic acid 290:20:1:1 or 290:50:1:1, respectively. From fractions of the dichloromethane extract, chavibetol, sitostenone, asperphenamate, daucosterol, 5,7-dimethoxy-aristolochic acid II and 10-amino-5,7-dimethoxy-aristolic acid II were later isolated, the last one was detected by Dragendorff reagent. From fractions of the methanol extract, glucosides of aristolic acid and of vanillic acid were isolated and separated by preparative TLC with chloroform – methanol – acetone – formic acid 450:75:25:2. This preparative TLC yielded protocatechuic acid (hRF 51) and a mixture (hRF 14–19), from which a methyl-phloroglucinol glucoside was isolated by gel exclusion chromatography.

J. Sep. Sci. 41, 518-524 (2018). HPTLC of withanolide S in the leaves of Withania somnifera on silica gel with dichloromethane – toluene – methanol – acetone 15:2:1:1. Detection by spraying with sulfuric acid reagent (180 mL methanol with 20 mL sulfuric acid) followed by heating at 120 °C for 10 min. Quantitative determination by fluorescence measurement at 365/>400 nm. The hRF value for withanolide S was 7. Linearity was between 15 and 500 μg/mL. LOD and LOQ were 30 and 92 μg/mL. The intermediate/interday/intra-day precisions were below 2 % (n=3). Recovery was in the range of 94.6 and 99.1 %.

Planta Medica 83(14/15), 1149-1158 (2017). The cyclodextrane fractionation of the methanolic percolate of Salix reticulata aerial parts was analyzed on silica gel with ethyl acetate – methanol – water 100:13:10. Detection by derivatization with vanillin-sulfuric acid reagent. Luteolin and apigenin glycosides, catechin, procyanidins and phenolic glucosides (picein, triandrin, and salicortin) were isolated from these fractions through preparative HPLC._x000D_

J. Ethnopharmacol. 233, 141-147 (2019). HPTLC of gentiopicrin in the dried roots and rhizomes of Gentiana scabra on silica gel with ethyl acetate – ethanol – water 8:2:1. Detection by spraying with chromogenic reagent (acetic acid – sulfuric acid – p-anisaldehyde 100:2:1). Qualitative evaluation at UV 254 nm. The hRF value for gentiopicrin was 58.

Phytochemistry 23, 2907-2910 (1984). Separation of very polar saponins from zexmenia buphthalmiflora by TLC on silica with chloroform - methanol - acetic acid - water 30:8:1.5:1. Detection with anisaldehyde/sulfuric acid. Solvent system also suitable for other saponins containing uronic acids. Also TLC of methylated saponins on silica with toluene-ethanol 20:7.

Phytochemistry 26, 229-235 (1987). TLC of complex triterpenoid saponin mixtures on silica with chloroform - methanol - formic acid - water 15:9:1:2 and ethyl acetate - methanol -water 8:1:0.1, on RP-8 with 60 % methanol and on Avicel with the upper layer of butanol -pyridine -. water 6:2:3. Detection with 5 % sulfuric acid - methanol. Also PLC and column chromatography. Solvent systems suitable for very polar saponins.