60th Indian Pharmaceutical Congress PA-221 (2008). HPTLC of terpenes in stem of Ocimum basilicum on silica gel with toluene - ethyl acetate 19:1. Evaluation of 5 spots under UV 254 nm and under visible light after treatment with anisaldehyde reagent followed by heating at 100 °C for 5 min: borneol (yellow, hRf 24), menthol (red, hRf 27), eugenol (blue, hRf 50), thymol (violet, hRf 57), safrole (blue, hRf 93).

in vitro culture. Pharmazie 64, 694-696 (2009). Preparative TLC and HPTLC of protocatechuic acid, vanillic acid, syringic acid, and p-coumaric acid and methanolic extracts on silica gel. Detection under UV light at 254 nm.

J. Planar Chromatogr. 22, 385-387 (2009). Ultrathin-layer chromatography of quercetin, rutin, and quercetin-3-O-glucoside on monolithic silica gel (size 30 mm x 18 mm) with binary and tertiary mobile phases in a cylindrical glass chamber previously saturated for 1 min. The migration distance was 20 mm and development time was 2 min. The investigated mobile phases were ethyl acetate - n-hexane 1:4 and 3:7, tetrahydrofurane - hexane 2:3 and 3:2, tetrahydrofurane - methanol - hexane 3:3:4, and hexane - acetone - methyl ethyl ketone 3:3:4. The best separation was achieved with acetone - methyl ethyl ketone - hexane 3:4:3. Densitometric evaluation at 366 nm.

J. Planar Chromatogr. 22, 359-362 (2009). HPTLC of aloenin in aloe juice, tablets, and liquid extracts on silica gel with ethyl acetate - 95 % ethanol - water 20:3:1 at room temperature in a saturated chamber. Detection by immersion for 1 s in freshly prepared 5 % sodium hydroxide solution in 95 % ethanol, followed by heating at 100 °C for 5 min. Quantitative determination by absorbance measurement at 365 nm.

J. Planar Chromatogr. 22, 305-307 (2009). TLC of diosgenin in methanolic extracts of fresh leaves and bulbs on silica gel with n-hexane - acetone 4:1. Detection by spraying with 25 % sulfuric acid in methanol and heating at 100 °C for 2 min. Quantitative determination by absorbance measurement at 540 nm.

J. Planar Chromatogr. 23, 46-49 (2010). HPTLC of polysaccharides (with galactose, glucose, mannose, arabinose, ribose, xylose, rhamnose, galacturonic acid, and glucuronic acid as standards) before and after hydrolysis on silica gel with chloroform - n-butanol - methanol - water - acetic acid 9:25:10:3:3 with chamber saturation for 30 min at room temperature. Detection by spraying with aniline - diphenylamine - phosphoric acid reagent and heating at 130 °C for 10 min. Quantitative determination by absorbance measurement at 380 nm. Ninhydrin reagent was used for detection of other compounds.

J. Planar Chromatogr. 23, 233-236 (2010). HPTLC of trans-citral and cis-citral in lemongrass oil on silica gel with toluene - ethyl acetate 17:3 in a twin-trough chamber saturated for 15 min (at 25 °C and 55 % RH). Detection by spraying with vanillin-sulfuric acid reagent. Quantitative determination by absorbance measurement at 595 nm. Intra-day and inter-day precision were evaluated by replicate (n = 6) analysis of samples (trans-citral at 450, 900, and 1800 ng/band, and cis-citral at 470, 940, and 1880 ng/band). The linear range was 225-3600 ng/band for trans-citral, and 470-3760 ng/band for cis-citral. The correlation coefficient r was 0.9933 for trans-citral and 0.9937 for cis-citral. Intra-day precision (n = 6) was < 3.56 and 5.66 % for trans- and cis-citral, respectively. Inter-day precision was assessed to be < 3.47 and 5.52 % for trans- and cis-citral by repeating the intra-day assay on three different days. Repeatability of sample application and peak-area measurement was 0.98 %, determined by performing six replicate analyses of the same band (1800 ng/band trans-citral and 1880 ng/band cis-citral). The RSD of recovery of trans and cis-citral was in the ranges 1.36-3.25 and 1.64-3.47, respectively.

Abstract No. C-37, 61st IPC (2009). HPTLC of methanolic leaf extracts of Aloe vera (after purification with petroleum ether (60-80 °C)) on silica gel with toluene - ethyl acetate - glacial acetic acid - methanol 4:18:1:4. Under UV 254 nm six bands with hRf values of 12, 26, 34, 44, 62, and 84 were observed. These bands correspond to well known constituents of Aloe vera: aloeresin hRf 25, barbaloin hRf 33, aloe emodin hRf 43, emodin hRf 63. The bands with hRf values of 12 and 84 could not be identified. The reported finger print profiling can serve as potential technique for authentification and batch to batch consistency of herbal drugs.