J. Planar Chromatogr. 24, 507-512 (2011). HPTLC of flavonoids (quercetin and kaempferol) and biflavonoids (sciadopitysin, ginkgetin, and bilobetin) in aqueous methanolic extracts of Ginkgo biloba on RP-18 by double development with 1) acetonitrile - water - methanol - formic acid 20:20:1:0.005 and 2) acetonitrile - water - methanol - formic acid 20:17:1:0.005. Quantitative determination by densitometry in absorption mode at 254 nm. LOD and LOQ were in the range of 0.12-0.37 and 0.60-1.85 µg/zone, respectively. Linearity was found over the concentration range of 0.5-4.0 µg/zone for quercetin, kaempferol, sciadopitysin, ginkgetin, and bilobetin with correlation coefficients r = 0.9990, 0.9922, 0.9939, 0.9974, and 0.9706, respectively. Average recoveries were in the range of 97.7-100.4 %.

J. Liq. Chromatogr. Relat. Technol. 34, 1925-1937 (2011). HPTLC of p-hydroxy benzoic acid (1), chrysoplenol-D (2), p-methoxy benzoic acid (3) and casticin (4) in the aerial parts of Vitex trifolia on silica gel with chloroform - methanol 24:1 + 1 drop formic acid. Quantitative determination by absorbance measurement at 254 nm. The hRf values of compounds (1) - (4) were 25, 31, 63 and 87, respectively. LOD and LOQ were found to be 18-58 ng/zone for (1), 39-132 ng/zone for (2),15-52 ng/zone for (3) and 33-111 ng/zone for (4). Repeatability and reproducibility (%RSD) for (1)-(4) were found in the range of 0.8-1.2 % and 1.2-1.3 %, respectively. Recoveries were obtained in the range of 94.0-101.1 %, 97.8-102.0 %, 95.1-100.1 %, and 97.6-100.3 % for compounds (1) - (4), respectively.

J. Planar Chromatogr. 25, 320-325 (2012). HPTLC of ursolic acid in the leaves of Diospyros melanoxylon on silica gel with chloroform - methanol 19:1. Detection by dipping in 5 % methanolic sulphuric acid reagent and heating at 105 °C for 3 min. Quantitative determination by absorbance measurement at 540 nm. Linearity was in the range of 50-450 ng/zone for ursolic acid. Limits of detection and quantification were found to be 20 and 40 ng/zone. Recovery (by standard addition) was 97.5 %.

J. Planar Chromatogr. 25, 426-432 (2012). HPTLC of shanzhiside methyl ester (1) and barlerin (2) on silica gel with chloroform - methanol 4:1. Quantitative determination by absorbance measurement at 240 nm. The hRf of compounds (1) and (2) were 30 and 48, respectively. Linearity was in the range of 200-1000 ng/zone. Limits of detection and quantification were found to be 13 and 22 ng/zone for (1) and 18 and 31 ng/zone for (2), respectively. Recovery was found to be 99.2-99.5 % for (1) and 98.9-99.2 % for (2), respectively. The method showed comparable results to a validated HPLC method.

J. of Chinese Med. 27 (166), 343-344 (2012). Baizhuhugan compound oral liquid is effective for enriching the blood, tonifying spleen, soothing the liver, and is clinically prescribed to cure chronic hepatitis and cirrhosis. For quality control, identification of the three contained herbal drugs by TLC of the extracts of the preparation 1) for Chinese Angelica, on silica gel with n-hexane – ethyl acetate 9:1, detection at UV 366 nm; 2) for Bighead Atractylodes rhizome, on silica gel with cyclohexane – ethyl acetate 7:3, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 3) for Chinese herbaceous Peony, on silica gel with chloroform - ethyl acetate – methanol – ammonia 8:1:4:1, detection by spraying with 5 % vanillin in ethanol – sulfuric acid 4:1 and heating mildly, viewing in daylight.

J. Planar Chromatogr. 25, 30-35 (2012). HPTLC of rutin (1), narcissin (2), nicotiflorin (3) and isoquercitrin (4) on silica gel with ethyl acetate - 1,2-dichloroethane - acetic acid - 85 % formic acid - water 100:25:10:10:8. Quantitative determination by absorbance measurement at 360 and 387 nm. Intraday and interday precisions were in the range of 1.5-1.9 % and 1.6-1.9 %, respectively. Recovery was found to be 98.2-101.4 % for (1), 98.0-101.1 % for (2), 98.2-100.5 % for (3) and 98.1-101.9 % for (4).

J. of China Pharm. 21 (10), 37-39 (2012). Changyanning oral liquid is a herbal TCM preparation with Euphorbia humifusa Willd as the key component drug. For quality control identification of Euphorbia humifusa Willd by TLC and quantification of its active component gallic acid by HPLC. TLC on silica gel with toluene – ethyl acetate – formic acid 5:2:1, detection by spraying with 1 % aluminium chloride in ethanol, evaluation under UV 366 nm.

J. of China Pharm. 21 (10), 26-27 (2012). Yifeitongluo granules are a herbal TCM preparation for the combined treatment of deficiency of both qi and yin. TLC of the extracts of the preparations on silica gel 1) for Rhizoma Polygonati, with chloroform – methanol – acetic acid 5:4:1, detection by spraying with 2.5 % phosphomolybdic acid in ethanol and heating at 105 °C until the zones are visible; 2) for Viola yedonensis Makino, with toluene – ethyl acetate – formic acid 5:3:1, detection under UV 366 nm; 3) for Stemona sessilifolia (Miq.) Miq, with toluene – propanone – methanol 16:6:1 after preconditioning with ammonia vapors, detection by spraying with 5 % potassium iodobismuthate and viewing in daylight.