Chinese J. of Guide for Trad. Chinese Med. & Pharm. 18 (7), 78-79 (2012). Zhilou fumigating lotion is a herbal TCM preparation for treatment of external hemorrhoids and hemorrhoid postoperation hemorrhage. TLC of the extracts of the preparation 1) for Rheum officinale, on silica gel with the upper phase of petroleum ether (30-60 ºC) – ethyl formate – formic acid 15:5:1, detection at UV 366 nm; 2) for Rhizoma Polygoni Cuspidati, on silica gel with chloroform – acetone – formic acid 16:3:1, detection at UV 366 nm; 3) for Chinese gall, on silica gel with chloroform – formyl acetate – formic acid 5:5:1, detection at UV 254 nm; 4) for Herba Houttuyniae, on silica gel with toluene – ethyl acetate – formic acid – water 20:10:1:1, detection at UV 366 nm; 5) for Salvia plebeia R.Br., on polyamide phase with methanol – acetic acid – water 13:3:4, detection at UV 366 nm.

J. of Xinjiang Trad. Chinese Med. 30 (3), 70-72 (2012). Compound Xuelian tablet is a herbal TCM preparation prescribed clinically to cure rheumatism. TLC of the extracts of the medicine on silica gel 1) for Saussurea involucrata, with the upper phase of ethyl acetate – formic acid – water 10:1:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight; 2) for Rhizoma Corydalis, with cyclohexane – chloroform – methanol 10:6:1, detection by exposure to iodine vapors until the zones are visible, evaluation in daylight and under UV 366 nm; 3) for Radix Angelicae Pubescentis, with petroleum ether (30-60 ºC) – formyl acetate – formic acid 15:5:1, detection under UV 366 nm.

Chinese J. Mod. Drug Appl. 6 (12), 131-132 (2012). Shisiwei Dangui Huoxue mixture is a herbal TCM for promoting blood circulation and relieving pain, and is prescribed clinically to treat blood stasis, local swelling caused by lesion of main and collateral channels of the injured limb after catagma. For quality control, TLC on silica gel 1) for Angelica sinensis, with n-hexane – ethyl acetate 9:1, detection at UV 366 nm; 2) for Radix Paeoniae Alba, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 followed by mild heating and evaluation in daylight.

Chinese J. of Lishizhen Trad. Med. & Pharm. 22 (4), 860-862 (2011). Fallopia multiflora (Thunb.) Harald, a medicinal herbal drug widely applied in traditional Chinese medicines, is available from different provinces of China. Presentation of fingerprint methods of Fallopia multiflora from different regions by micellar thin-layer chromatography (MTLC) and IR techniques. MTLC of methanol extracts of Fallopia multiflora and emodin standard solution on polyamide layer with 0.4 % cetylpyridinium chloride in acetone – methanol – 1.0 mol/L NaOH 12:4:5 after chamber saturation for 15 min. Detection of the purple zones in daylight. The ratio of the hRf values of the component zones and those of emodin was calculated. In addition, FTIR of the methanolic extracts and emodin standard solution mixed in KBr tablets. The results of the real life samples available from five different regions are given and proved that the method is suitable for the quality control of the drugs.

by thin-layer chromatography) (Chinese). J. of Trad. Chinese Med. & Pharm. Consult. 4 (5), 481-482 (2012). As a widely used TCM herbal drug, Polygonum orientale Linn. is prescribed for treatment of rheumatic arthritis, coronary heart disease, stomach pain, etc. Description of a method for identifying the counterfeit drug and for routine quality control of the crude drug by identification of the flavonoids quercetin and kaempferol in the drug samples obtained by extraction of the dry stem, leaves and fruits of the herb after acid hydrolysis. TLC on silica gel with toluene – ethyl acetate – formic acid 20:14:1, detection by spraying with 1 % aluminium chloride in ethanol and heating mildly, viewing under UV 366 nm.

Jiangxi J. of Trad. Chinese Med. 43 (354), 62-64 (2012). Jiegu Xujin capsules are a herbal TCM for the treatment of fractures, bone dislocation and joint pain. For quality control, TLC on silica gel 1) for Rhizoma Drynariae and the standard naringin, with cyclohexane – ethyl acetate – formic acid – water 2:24:5:6, detection under UV 366 nm; 2) for Radix Angelicae Sinensis and Rhizoma Ligustici Chuanxiong, with n-hexane – ethyl acetate 9:1, detection under UV 366 nm; 3) for Eupolyphaga seu steleophaga, with toluene – dichloromethane – acetone 10:10:1, detection by spraying with 3 % vanillin in sulfuric acid – ethanol 1:200 and heat at 105 °C, viewing in daylight; 4) for Radix Notoginseng and the standards ginsenoside Rb1, Re, Rg1, R1, with the lower phase of chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing under UV 366 nm.

Chinese J. of Jilin Med. Col l. 33 (1), 8-10 (2012). Citrus limon is a class of traditional Chinese medicinal plants used in various TCM preparations. To guarantee the safety and effectiveness it is significant to identify the authenticity of the crude drugs. TLC of 11 varieties of standard crude drug 1) for alkaloids with the standard synephrine, on 0.5 % NaOH containing silica gel with chloroform – ethanol – ammonia 5:5:1, detection by spraying with 1 % ninhydrin in ethanol and heating at 105 °C, viewing under UV 366 nm; 2) for flavonoids with the standard hesperidin and naringin, on silica gel with ethyl acetate – methanol – water 100:17:13, detection by spraying with 1 % aluminiumchloride in ethanol and viewing under UV 366 nm. The method demonstrated the merits of TLC, such as high throughput, fast analysis, no interferences, specificity, intuitive use and can be a base of establishing the operational standard method for the quality control of the crude drugs.

J. of Qilu Med. & Pharm. 31 (4), 574-576 (2012). Fufang Jugeng Mahuangjian Tangjiang syrup ll is a herbal TCM preparation for treating cough caused by asthmatic bronchitis. For quality control and to guarantee the safty and effectiveness of the medicine, TLC on silica gel 1) for ephedrine hydrochloride, with n-butanol – formic acid – water 4:1:5, detection by spraying with 0.2 % ninhydrin in acetone and heating at 105 °C, viewing in daylight; 2) for Platycodon grandiflorus, with chloroform – diethyl ether 1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight.