J. Chromatogr. Sci. 56, 518-523 (2018). Presentation of a validated method for the quantitation of dihydrokaempferol-4′-O-glucopyranoside. This flavanonol glucoside is a marker compound in the flower of Alcea rosea L. with significant antioxidant and anticancer activity against the HepG-2 cell line. HPTLC on silica gel with ethyl acetate – methanol – water – acetic acid 600:100:80:3 over 70 mm with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 295 nm. The amount of dihydrokaempferol-4′-O-glucopyranoside in the flowers of A. rosea was 0.733 g/100 g and 0.928 g/100 g after maceration and sonication for 15 min, respectively. Linearity was in the concentration range of 0.9-3.6 µg/zone. The %RSD of the intra-day and inter-day precision was 0.2–1.5 % and 0.5–1.7 %, respectively. The LOD and LOQ were 312 ng/zone and 947 ng/zone, respectively.

Pharmacogn. Mag. 14, 45-51 (2018). HPTLC of stigmasterol in Monochoria vaginalis and Monochoria hastata on silica gel with chloroform – methanol 4:1. Detection by spraying with anisaldehyde-sulfuric acid reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 540 nm. The hRF value for stigmasterol was 30. Linearity was between 1000 and 5000 ng/zone. LOD and LOQ were 80 and 200 ng/zone. The intermediate precision was <3 % (n=3). Average recovery was 99.8 %.

J. Chromatogr. 312, 357-385 (1984). Two-dimensional TLC of dansylated basic nitrogen fractions (BNF) on silica with cyclohexane - ethyl acetate 2:3 and with benzene - triethylamine 5:1. Detection by UV 366 nm. Dansylation of the plant amines was carried out prior to TLC with DNS chloride in acetone (5 mg/ml) in the presence of excess solid NaHCO3. Standards for comparison were separated with chloroform - butylacetate 8:3 and with cyclohexane - benzene - methanol 2:7:1. Rf values of more than 80 plant amines are reported.

Phytochemistry 25, 2910-2912 (1986). Separation of indole alkaloids and heteroyohimbine alkaloids on silica with chloroform - methanol 9:1, ethyl acetate - isopropanol - NH3 16:3:1 or chloroform - acetone 5:4. Detection with Dragendorff's reagent for alkaloids, 0.2 M FeCl3 in 35 % perchloric acid for heteroyohimbine alkaloids, 0.5 % anisaldehyde in formic acid - sulfuric acid - methanol 2:1:17 for terpenes and procyanidins and with 1 % vanillin in 50 % aq. phosphoric acid for alkaloids and terpenes. Preparative Separation by centrifugal TLC on silica with chloroform - methanol 9:1 and 4:1.

Planta Med. 57, 560-565 (1991). TLC of fourteen bufadienolides from a bulb extract of Urginea maritima on silica with chloroform - methanol - water 140:44:7 and ethyl acetate - methanol - water 81:11:8. Also HPTLC on RP-2 with methanol - water 1:1. Detection by spraying with anisaldehyde/sulfuric acid. Excellent separation of cardiotonic glycosides on RP-2 HPTLC plates. Also CC and HPLC.

CBS 90, 6-7 (2003). HPTLC phospholipids and glycolipids from rape seed on lichrospher silica gel with chloroform - methanol - acetone - water 18:15:2:1 for phospholipid separation and with acetone - chloroform - water 30:15:2 for glycolipid separation, developing distance 70 mm each. Detection by dipping in molybdatophosphoric acid reagent (5 % in ethanol) followed by heating at 120 °C for 15 min. Quantitative determination by absorbance measurement at 720 nm.

68, 790-793 (2002). Preparative and analytical TLC of melisemine, confusadine, melicarpinone, edulinine, (S)-(-)-7,8-dimethoxyplatydesmine, isoplatydesmine, skimmianine, confusaneline, kokusaginine, and haplopine on silica gel with chloroform - acetone 5:1 and 10:1, chloroform - methanol 25 :1 and 3:1, benzene - ethyl acetate 1:1, benzene - methanol 10:1, and ethyl acetate - methanol 10:1.

Planta med. 70, 152-159 (2004). Analytical HPTLC on silica gel RP-18 and preparative TLC on silica gel of piperine, piperamine, piperlonguminine, and methyl piperate with n-hexane - ethyl acetate 1:1. Detection by spraying with 1 % cerium sulfate - 10 % aqueous sulfuric acid followed by heating on a plate heater.