Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      105 095
      Estimation of ellagic acid in Eugenia jambolana Lam seed alcoholic extract by HPTLC method
      N. PATEL*, D. MODI, B. SHAH, P. RACHH (*Vidyabharati Trust College of Pharmacy, Surat, Gujarat, India)

      Abstract No. C-497, 61st IPC (2009). HPTLC of ellagic acid in seeds of Eugenia jambolana Lam on silica gel with ethyl acetate - glacial acetic acid - formic acid - water 100:11:11:27. Densitometric evaluation at 254 nm. The method was linear in the range of 200-1200 ng/band. The alcoholic seed extract contained 11.03 % of ellagic acid and 21 % of total tannin (measured by chemical method).

      Classification: 32e
      106 024
      Quality control standards for the roots of three plumbago species
      S. ARIYANATHAN*, A. SARASWATHY, G. RAJAMANICKAM (*Centre for Advanced Research in Indian system of Medicine (CARISM) SASTRA University, Thanjavur 613402, India, saraswathy20042000@yahoo.co.in)

      Ind. J. Pharma. Sci. 72(1), 86-91 (2010). Three species of Plumbago (Plumbaginaceae), i.e. P. zeylanica, P. carpensis, and P. rosea were studied for different physico-chemical parameters in addition to the estimation of microbial contamination, aflatoxins and pesticide residues and heavy metal content. All three species are used as herbs. The fingerprint profile of each species was compared using plumbagin as marker. Chloroform extracts of each plant were subjected to chromatography on silica gel with toluene - ethyl acetate 4:1 in a saturated twin trough chamber. Detection under UV 254 nm and 366 nm. The hRf value of plumbagin was 70. The identity of plumbagin in the samples was shown by overlay of the UV spectra. Linearity was between 200 and 1000 ng/zone. The amount of plumbagin in the three species was between 0.01 and 0.17 %.

      Classification: 6
      106 078
      Quantification of beta-sitosterol from three Jatropha species by high-performance thin-layer chromatography
      Rani BHAGAT, D. KULKARNI* (*Botany Group, Agharkar Research Institute, Pune 411004, India, dilipkkulkarni@gmail.com)

      Asian Journal of Chemistry 22(10), 8117-8120 (2010). HPTLC of beta-sitosterol in leaves, roots and seed oil of 3 species of Jatropha. Chromatographic separation on silica gel with toluene - methanol 9:1. The hRf value of beta-sitosterol was 54. Derivatization with anisaldehyde-sulfuric acid reagent, followed by heating at 120 °C. After derivatzation densitometric evaluation at 525 nm. The method was linear in the range of 100-500 ng/band. The proposed method was used for the estimation of beta-sitosterol in extracts of different species of plants. The presence of beta-sitosterol in samples was confirmed by multi-wave length scanning.

      Classification: 15a
      106 099
      Quantification of beta-carotene from Diplocyclos palmatus jeff
      A. PATIL*, Darshana PATIL, A. SHARMA, N. CHANDRA (*Dept. of Botany-Herbal Sciences, Birla College, Kalyan 421304,India, dravinashpatil@rediffmail.com)

      fruits rind by using high-performance thin-layer chromatography. Asian Journal of Chemistry 23(2), 788-790 (2011). A simple HPTLC method has been developed for estimation of beta-carotene in fruit rind of Diplocyclos palmatus (Cucurbitaceae). The rind of fruits was extract with acetone. HPTLC on silica gel with petroleum ether as mobile phase. The hRf value of beta-carotene was 30. Densitometric evaluation at 450 nm. The method was linear in the range of 6-60 ng/band. The recovery was 99.4 % for beta-carotene.

      Classification: 17a
      106 157
      Identification and separation of Cannabis sativa, Embleia ribes, Myristica fragrans and Piper longum from organic extract on silica gel surface with anionic micellar solvent system
      A. MOHAMMAD*, S. SHARMA, S. BHAWANI, R. SINGH (*Analytical Research Lab., Dept. of Applied Chem., Faculty of Eng. & Tech., Aligarh Muslim University Aligarh 202002, India)

      The Open Nutraceuticals Journal 2, 2-6 (2009) A TLC method using a micellar solution of sodium dodecyl sulfate (SDS) as mobile phase has been developed for identification of four herbals present in Jatiphaladya, a powdered herbal formulation containing Cannabis sativa, Myristica fragrans, Piper longum, and Embleia ribes. The formulation was extracted with 80 % ethanol. TLC on laboratory made plates coated with silica gel and activated at 100 °C for 60 min, with a 5 % solution of SDS as mobile phase. The resolved spots were identified by spraying with a 2 % solution of vanillin in 5 % methanolic sulfuric acid. Spots corresponding to different herbals were well resolved. Different detection reagents were evaluated, i.e. iodine, vanillin sulfuric acid, and anisaldehyde-sulfuric acid. Vanillin sulfuric acid reagent was found to be the most sensitive. Of the different surfactants used, anionic, cationic and nonionic, SDS was found to be most suitable. The most suitable pH of the mobile phase was pH 4.2-5.7, it provided optimum resolution of zones.

      Classification: 32e
      107 044
      Quantification of p-(para)methoxy cinnamic acid ethyl ester (PMCAEE) from Hedychium spicatum by HPTLC
      R. ARORA*, S. JAIN (*Noida Institute of Engineering & Technology Dept. of Pharmaceutical Sciences 19, Knowledge park, Phase 2 Greater Noida, Uttar Pradesh, India, ritu.wadhwa84@gmail.com)

      International Journal of Pharma and Bio Sciences 1(3), 1-4 (2010). The presence of p-methoxy cinnamic acid ethyl ester (PMCAEE) in Hedychium spicatum (Zingiberaceae), a spicy annual herb, was confirmed by TLC and other qualitative tests. HPTLC of PMCAEE on silica gel with n-hexane – acetone 4:1. The hRf value of PMCAEE was 43. Quantitative determination by absorbance measurement at 310 nm. The method was linear in the range of 1-5 µg/band. The alcoholic extract of the plant was found to contain 0.81 % of PMCAEE.

      Classification: 11a
      107 124
      HPTLC method for estimation of isolated derivative in fractions of seeds of Ensete superbum
      M. KACHROO*, S. AGRAWAL (*Dept. of Pharmaceutical Chemistry, Al-Ameen College of Pharmacy, Hosur Rd., Bangalore 560027, India)

      J. Chem. Pharm. Res. 2(1), 155-161 (2010). A chroman derivative (C16O4H22) was isolated from the ethanolic extract of dried seeds of Ensete superbum. HPTLC on silica gel with toluene – ethyl acetate – formic acid 5:4:1. Quantitative determination by absorbance measurement at 254 nm. The linear range was 300-900 ng/band. The amount of the chroman in different fractions of the extract was 1.83 % (ethanol fraction), 1.74 % (ethyl acetate fraction) and 0.74 % (methanol fraction).

      Classification: 32e
      108 012
      The start-to-end chemometric image processing of 2D thin-layer videoscans
      L. KOMSTA*, L. CIESLA, Anna BOGUCKA-KOCKA, Aleksandra JÓZEFCZYK, J. KRYSZEN, Monika WAKSMUNDZKA-HAJNOS (*Dep. of Med. Chem., Med. Univ. of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland)

      J. of Chromatogr. A 1218 (19), 2820-2825 (2011). A unified procedure for image preprocessing of 2D TLC videoscans saved as JPG files is proposed for further supervised or unsupervised chemometric analysis. The procedure was based on open source software and included denoising using a median filter, baseline removal with the rollerball algorithm and nonlinear warping using spline functions. The application of the proposed procedure enabled filtration of random differences between images, such as changes in the intensity of the background as well as differences in the location of the zones. After the preprocessing only the zone intensity had an influence on the statistical analysis by principal component analysis (PCA) or other techniques. The proposed technique was successfully applied for the determination of the differences between three Carex species based on the 2D videoscans of the extracts.

      Classification: 3f
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