Planta Medica 82, 09/10, 903-909 (2016). To monitor the sorbent phase purification of a chloroform subfraction of the sponge Myxilla incrustans, TLC of fractions on silica gel with dichloromethane – methanol – formic acid 900:100:1, detection with Dragendorff’s reagent. The four positive fractions were purified by preparative HPLC, yielding three new N-acyl dopamine/dehydrotyrosine glycosides: myxillins A, B and C._x000D_

Chinese J. Hosp. Pharm. 35 (11), 992-996 (2015). Sisheng Keli granule is a herbal TCM preparation for treating hematemesis, hematuria, allergic purpura and thrombocytopenic purpura, etc. For quality control, TLC on silica gel (1) for Artemisia argyi Levl. et Vant., with petroleum ether (60-90 °C) – toluene – acetone 20:16:1, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C until the zones are visible; (2) for Platycladus orientalis (L.) Franco, with toluene – ethyl acetate – formic acid 5:2:1, detection by spraying with 1 % aluminium trichloride in ethanol and detection under UV 366 nm; (3) for Lotus leaf, with chloroform – acetone – 10 % ammonia in ethanol 60:20:1, detection by spraying with 5 % potassium bismuthate solution and heating at 105 °C until the zones are visible. Quantification of nuciferin and verbascoside by HPLC.

J. Planar Chromatogr. 29, 336-340 (2016). HPTLC of isoalantolactone (1), germacranolide (2), β-sitosterol (3), and lupeol (4) in the roots of Inula cappa on silica gel with toluene ‒ methanol 47:3. Detection by dipping into anisaldehyde reagent followed by heating at 105 ºC until colored bands appeared. Quantitative determination by absorbance measurement at 525 nm. Linearity ranged from 10-70 μg/mL for (1), 10-60 μg/mL for (2), 8-48 μg/mL for (3) and 15-90 μg/mL for (4). The intermediate precisions were below 0.9 % (n=7). The LODs and LOQs were 5 and 10 μg/mL for (1), (2) and (4) and 2 and 6 μg/mL for (3). Average recoveries were between 98.3 and 99.0 % for (1) to (4).

J. Ethnopharmacol. 195, 10-19 (2017). HPTLC of mesembrine alkaloids (mesembrine, mesembrenone, mesembrinol, mesembrenol, epimesembranol, epimesembrenol) in the South African medicinal plant Sceletium tortuosum on silica gel with dichloromethane – methanol – 10 % ammonia 900:100:1. Quantitative determination by absorbance measurement at 280 nm. The LOD and LOQ were in the range of 18-31 ng/zone and 44-95 ng/zone, respectively.

Part II: Remijia ferruginea. Rev. Bras. Farmacogn. 27, 153-157 (2017). HPTLC fingerprint of Remijia ferruginea on silica gel with ethyl acetate – toluene – formic acid – water 12:4:4:3. Detection by spraying with natural product reagent, followed by PEG reagent. The hRF values for rutin, chlorogenic acid and cinchonine were 50, 60 and 65, respectively.

Planta Med. 83(03/04), 334-340 (2017). Preparative layer chromatography on silica gel was used to isolate, from flash column subfractions of ethyl acetate extracts of Lepisanthes senegalensis stem or roots: a) with chloroform – ethyl acetate 3:37 an acetyl-caffeoylbutelin from stem; b) with hexane – ethyl acetate 4:1 two coumaroyl-22-hydroxy-hopanes from root; c) with dichloromethane – ethyl acetate 19:1 a caffeoyl-lupeol from root.

J. Chromatogr. A 1526, 137-150 (2017). HPTLC of physalins from crude extracts of Chinese lantern (Physalis alkekengi L.) on silica gel with ethyl acetate – toluene – formic acid 35:15:1. Densitometric screening of physalins in absorption and in fluorescence mode after post-chromatographic derivatization with sulfuric acid reagent. Identification of the physalin L standard and its impurity, 2,3,25,27-tetrahydrophysalin A. Applying two successive plate pre-developments with methanol – formic acid 9:1 and methanol to avoid strong ion suppression caused by the developing solvent additive (formic acid), and to improve the sensitivity of HPTLC-MS/MS method combined with a slightly modified developing solvent ethyl acetate – toluene – formic acid 30:20:1. Non-targeted characterization of physalins from the same chromatographic zone and determination of physalin types by simultaneous hyphenation of HPTLC with a triple quadrupole and an ion trap mass analyzer. Demonstration of the performance of the HPTLC-densitometric and HPTLC–MS/MS methods for the analysis of physalins from aqueous reflux extracts. Observation of variations in physalin profiles and abundances in different parts of P. alkekengi harvested at different stages of maturity, showed that the husks are the most suitable plant part for P. alkekengi quality control.

J. Planar Chromatogr. 30, 429-439 (2017). HPTLC of physalin L in orange husks of Physalis alkekengi L. var. franchetii on silica gel with ethyl acetate – n-hexane 3:2. Detection by dipping into 2.5 % (v/v) sulfuric acid in ethanol. Qualitative determination under UV 366 nm. The hRF values for physalin L and its impurity were 61 and 51, respectively, as determined by HPTLC-MS.