J. Planar Chromatogr. 22, 445-448 (2009). HPTLC of myristicin, safrole and extract of seeds on silica gel, prewashed with methanol, with toluene in an automatic developing chamber saturated for 20 min. Quantitative determination by absorbance measurement at 210 nm for myristicin and at 290 nm for safrole.

J. Chinese Trad. & Herb. Drugs 40 (8), 245-247 (2009). TLC of the TCM drug extracts on silica gel with 1) petroleum ether (90-120 °C) – toluene – formic acid 20:40:1; 2) chloroform – ethyl acetate – methanol – water – formic acid 3:10:2:2:2; 3) ethyl acetate – formic acid – water 12:2:3. Detection 1) by spraying with 10 % sulfuric acid in ethanol followed by heating at 105 °C; 2) by exposure to iodine vapor; 3) under UV 254 nm.

60th Indian Pharmaceutical Congress PG-258 (2008). HPTLC of isovitexin in Passiflora incarnata raw material and extract on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. The plant was found to contain 0.087 % w/w of isovitexin.

J. Planar Chromatogr. 22, 381-384 (2009). TLC of aloin in commercial Ayurvedic preparations on silica gel (prewashed with methanol) with ethyl acetate - methanol - water 50:7:2 in a twin trough chamber with chamber saturation for 5-7 min at 30 +/- 4 °C and a relative humidity of 57 +/- 3 %. Quantitative determination by absorbance measurement at 360 nm. The limit of detection and quantification was 10 and 20 ng/band, respectively.

Chinese J. Hospit. Pharm. 29 (4), 336-338 (2009). TLC of astragaloside in Tianjian capsules on silica gel with chloroform - methanol - water 13:6:2. Detection by spraying with 5 % sulfuric acid in ethanol followed by heating at 105 ºC until coloration. Quantification by densitometry at 515 nm. The linearity was between 0.98 and 4.90 µg/spot (r2 = 0.998), the %RSD was 2.4 % (n = 6) within plate and 2.3 % (n = 6) inter-plate, standard addition recovery was 99.2 % with RSD = 1.7 % (n = 6).

International Seminar on Herbal Drug Research, PN-009 (2009). Bioassay guided fractionation of Lagenaria siceraria was carried out on a silica gel column with solvents in ascending order of polarity. Each fraction obtained was subjected to preparative TLC on silica gel with n-butanol - methanol - water 3:1:1. Four bands with different Rf values were collected and active compounds were extracted and screened for antihyperlipidemic activity.

J. Planar Chromatogr. 23, 225-226 (2010). OPLC of oregano oil components (carvacrol, thymol, and linalool) on silica gel with dichloromethane. Detection under UV 254 nm, by spraying with vanillin-sulfuric acid reagent (0.1 g vanillin, 100 mL ethanol, and 2.2 mL 95-98 % sulfuric acid) and heating at 110 °C for 3 min, and in the BioArena system (the dried developed plates were dipped for 10 s into an aqueous cell suspension of the soil bacteria Bacillus subtilis and incubated for 2 h at 100 % rel. humidity and 30 °C). Visualizion of antimicrobial compounds was performed by immersing the plates for 5 s in an aqueous solution of MTT reagent (80 mg MTT and 100 mg Triton X-100 in 100 mL water). The layers were further incubated and and documented.

International Seminar on Herbal Drug Research, PN-051 (2009). HPTLC of four different extracts of rhizomes of Cyperus rotundus on silica gel with chloroform - benzene 1:1. Evaluation under 254 nm. Detection by treatment with ethanolic potassium hydroxide and evaluation of the fingerprint under daylight.