Acta Chromatographica 22 (4), 651-663 (2010). HPTLC of wedelolactone (WED) and asiaticoside (ASI) in Eclipta alba and Centella asiatica Linn., respectively, on silica gel with toluene - acetone - methanol - formic acid 60:40:40:1. The hRf value was 26 and 75 for ASI and WED, respectively. Detection by spraying with 10 % methanolic sulphuric acid. Quantitative evaluation by absorbance measurement at 317 nm for WED and at 530 nm after derivatization for ASI. The linearity was in the range of 50-250 ng/band for WED and 150-550 ng/band for ASI with r = 0.999 for WED and 0.9989 for ASI. The recovery of WED was 99.3 % and of ASI 99.5 %.

Brazilian Journal of Pharmacognosy 21, 818-823 (2011). This review describes recent advances in HPTLC automatization as a useful tool for the analysis of complex mixtures of natural products. The author also compares HPTLC with TLC and HPLC. The review provides a general perspective for HPTLC fingerprint approach for the analytical determination of botanicals.

26th IPGA Annual conference, 12 (2010). TLC of berberine in plant extracts and methanolic solutions of a commercially available herbal formulation on silica gel with n-butanol - acetic acid - water 14:3:4. Densitometric evaluation at 435 nm. The method was found to be linear in the range of 8.1-26.9 ng/band. The proposed method was applied to the analysis of different formulations using berberine as chemical marker.

J. Strait Pharm. 22 (12), 59-60 (2010). TLC of the extracts of the title traditional Chinese medicine 1) for red Ginseng, on silica gel with benzene - ethyl acetate - formic acid 20:16:3, detection by spraying with 3 % FeCl3 in ethanol and viewing under daylight; 2) for Chinese Taxillus twig, on silica gel with water-saturated toluene - ethyl acetate - formic acid 5:4:1, detection by spraying with 5 % AlCl3 in ethanol; 3) for Hawthorn, on silica gel with toluene - ethyl acetate - glacial acetic acid 24:8:1, detection by spraying with 3-10 % sulfuric acid in ethanol and heating at 105 °C until the zones were detected; 4) for Alisma orientale, on silica gel with toluene - ethyl acetate - formic acid 14:7:2, detection by spraying with acetic acid - concentrated sulfuric acid - ethanol 1:1:1, heating at 105 °C until the zones were detected and viewing under daylight. Identification by comparison with the standards of the individual drug.

J. Liq. Chromatogr. Relat. Technol. 32, 2437-2450 (2009). HPTLC calliterpenone (1) and calliterpenone monoacetate (2) in the leaves of Callicarpa macrophylla on silica gel with methanol - water 9:11. Quantitative determination by absorbance measurement at 210 nm. The hRf values of (1) and (2) were 43 and 73, respectively. Linearity was between 1-5 µg/zone for (1) and (2). LOD and LOQ were 230 and 780 ng/zone for (1) and 220 and 730 ng/zone for (2). Intra-day and inter-day precisions ranged between 1.3-1.7 % for (1) and 1.1-1.7 % for (2). Recoveries (by standard addition) were between 97.5-100.8 % for both.

J. of Chromatogr. A 1241, 103-111 (2012). Development of a simple, fast, and efficient HPTLC method for the simultaneous quantitative determination of alcohols and acetates in Haitian vetiver essential oils (Chryzopogon zizanioides) and its acetylated form. TLC on silica gel with n-hexane – chloroform – ethyl acetate 16:12:1 at 47 % relative humidity. Detection with vanillin – sulfuric acid reagent. Quantification of the analytes by densitometry in absorbance mode at 530 nm. Preparation of the reference mixtures of alcohols and acetates by fractionation of Haitian vetiver oil or vetiver acetates, followed by purification of the fractions of interest by means of OPLC. Determination of the chemical composition of each reference fraction by using GC-MS and GC×GC-MS, and of their overall purity by HPTLC with good linearity (r2 = 0.9995 for alcohols, r2 = 0.9996 for acetates) in a concentration range of 40–200 ng/zone.

J. Planar Chromatogr. 25, 251-258 (2012). TLC of trans-resveratrol (1), pterostilbene (2), and p-coumaric acid (3) in samples of recombinant bacterial cultures, food supplements, and wine on silica gel with n-hexane - ethyl acetate - formic acid 20:19:1. Quantitative determination by absorbance measurement at 286 nm (3) and 303 nm for (1) and (2).

J. of China Pharm. 26 (5), 503-504 (2012). E Ling capsule is a herbal TCM for activating blood circulation to dissipate blood stasis, relieving pain, eliminating stagnation, and is prescribed clinically to treat pelvic endometriosis. For quality control, TLC of the extracts of the medicine on silica gel 1) for the root of red-rooted salvia, with toluene – ethyl acetate 19:1, detection by viewing in daylight; 2) for Fructus Aurantii, first over 4 cm with ethyl acetate – methanol – water 100:17:10, then over 8 cm with toluene – ethyl acetate – formic acid – water 20:10:1:1, detection at UV 366 nm after spraying with 1 % aluminium chloride in ethanol; 3) for Rhizoma Corydalis, with n-hexane – chloroform – methanol 15:8:2, detection at UV 366 nm.