Chinese J. Mod. Drug Appl. 7 (3), 132-133 (2013). Qinggan Lidan Jiaonang capsules are a TCM preparation for the treatment of cholecystitis, acute and chronic hepatitis, fatty liver, cirrhosis, etc. For quality control, TLC on silica gel (1) for Panax pseudoginseng Wall. var. notoginseng (Burkill) Hoo et Tseng and notoginsenoside R1 with chloroform – methanol – water 14:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible; (2) for Reynoutria japonica Houtt. and emodin with the upper phase of petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection (A) at UV 366 nm or (B) after exposure to iodine vapor, evaluation under white light; (3) for Natural Indigo, indigo and indirubin with benzene – chloroform – acetone 5:4:1, detection under white light.

Chinese J. of Pharm. Research 33 (6), 328-331 (2014). Yinyanghuo Tongluo Wan pill is a TCM preparation for special treatment of diabetic peripheral neuropathy. For quality control, TLC on silica gel (1) for processed Epimedium and the standard icariin with ethyl acetate – butanone – formic acid 10:1:1:1, detection by spraying with 3 % aluminum trichloride in ethanol and heating mildly until the zones are visible at UV 366 nm; (2) for Panax ginseng C. A. Mey., ginsenoside Rb1, ginsenoside Re, and ginsenoside Rg1 with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible and at UV 366 nm; (3) for Ligusticum chuanxiong Hort. with cyclohexane - ethyl acetate 9:1, detection at UV 366 nm; (4) for Paeonia lactiflora Pall. and paeoniflorin with chloroform – methanol – ethyl acetate – concentrated ammonia 20:8:4:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4, followed by heating until the zones are visible; (5) for Atractylodes macrocephala Koidz. with petroleum ether (60-90 °C), detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4, followed by heating until the zones are visible; (6) for Cassia twig with petroleum ether (60-90 °C) – ethyl acetate 17:3, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4, followed by heating at 105 °C until the zones are visible, and at UV 366 nm.

J. Sep. Sci. 38, 592-598 (2015). HPTLC of erythrocentaurin on silica gel with toluene - ethyl acetate - formic acid 40:9:1. Quantitative determination by absorbance measurement at 230 nm. The hRF value of erythrocentaurin was 54. Linearity was in the range of 200-1500 ng/zone. LOD and LOQ were 60 and 180 ng/zone, respectively. Intra-day and inter-day precision was below 1 % (n=3). Recoveries were between 97 and 99 %.

J. Planar Chromatogr. 28, 466-471 (2015). TLC of L-glutamic acid in a solution of Astragalus complanatus coarse powder on silica gel with n-butyl alcohol - glacial acetic acid - water 4:1:1. Detection by spraying with 0.2 % ninhydrin - acetone solution 10:1, followed by heating at 105 °C. Quantitative determination by measuring pixels after photographing. Linearity was in the range of 10-60 μg/zone. Average recovery was 103 %. Different measures to reduce the error were proposed.

J. Liq. Chromatogr. Relat. Technol. 38, 1794-1801 (2015). 2D-TLC of eleven essential oils from nine Mentha species on silica gel with ethyl acetate - toluene 5:19 in the first direction and ethyl acetate - n-heptane 3:17 in the second direction. Detection by dipping into p-anisaldehyde reagent (0.5 mL p-anisaldehyde solution in 50 mL glacial acetic acid and 1 mL concentrated sulfuric acid) followed by heating at 105 °C for 10 min. Evaluation under 254 nm. The developed plates were immersed in a 0.2 % methanolic solution of 2,2-diphenyl-1-picrylhydrazyl (DPPH radical reagent) and scanned after 30 min.

Planta Medica 82, 11/12, 1051-1057 (2016). The crude ethanol extract of the aerial parts of the liverwort Chiloscyphus polyanthos, its fractions and seven pungent sesquiterpene lactones isolated therefrom were analyzed against Candida albicans strains (hypersensitive mutant DSY2621, wild-type parent CAF2-1). 20 mL of C. albicans suspension were spread and solidified on the plate charged with samples (20 µg/spot), blank (methanol) or reference (miconazole); without any development, the plate was incubated overnight at 37 °C, sprayed with methyl thiazolyl tetrazolium chloride (MTT 0.25 %) and incubated for 6 h at 37 °C to obtain a purple background. Diplophyllolide A was active on both strains, four other lactones only on DSY2621 (l-diplophyllin, dihydrodiplophyllin, ent-3-oxodiplophyllin, 3β-hydroxyeudesma-4,11-dien-12,8α-olide)._x000D_

CBS 115, 5-7 (2015). HPTLC of xanthones in mangosteen fruit (Garcinia mangostana) on RP-18 with acetonitrile – water – formic acid 80:17:3 to a migration distance of 65 mm. Detection by spraying with NEU's reagent and PEG, evaluation under UV 366 nm. The hRf value of β-mangostin was 11, of 9-hydroxycalabaxanthone 14, of α-mangostin 29, and of γ-mangostin 43. Quantitative determination of the four xanthones as α-mangostin equivalents by absorbance measurement at 280 and 320 nm. Comparison with an HPLC-UV method on RP-18 showed only a small difference (1.5 %) of the quantified total content. HPTLC was found to be a good alternative to HPLC.

J. of China Pharm. 23 (2), 48-51 (2014). Qingbu Tongluo Wan pill is a herbal TCM preparation for the treatment of acute and chronic brucellosis. For quality control, TLC on silica gel (1) for Fructus aurantii, with chloroform – formic acid – water 13:6:2, detection by spraying with 3 % aluminium chloride in ethanol and heating at 105 ºC, detection under UV 366 nm; (2) for Salvia miltiorrhiza Bge., with petroleum ether (60-90 ºC) – ethyl acetate 4:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC, evaluation in daylight; (3) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu, with toluene – acetone 9:2, detection by exposing to iodine vapors for 3 min, evaluation under UV 365 nm; (4) for Radix paeoniae rubra, with chloroform – ethyl acetate – methanol – formic acid 200:25:50:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating mildly until the zonesare visible in daylight. Quantification of salvianolic acid B by HPLC.