J. Planar Chromatogr. 24, 172-175 (2011). HPTLC of arjunolic acid on silica gel, prewashed with methanol, with chloroform - toluene - ethanol 4:4:1 in a twin-trough chamber saturated with mobile phase for 15 min. Detection by spraying with anisaldehyde reagent followed by heating at 110 °C for 5-7 min. Quantitative determination by densitometry at 600 nm. Linearity was between 50 and 500 ng/band. The hRf value was 28. The inter-day, intra-day, and inter-analyst precision was (%RSD, n = 6) 0.2-0.5, 0.3-0.4, 0.2-0.9 %, respectively. The recovery was in the range of 98.1-101.8 %. The robustness (%RSD, n = 3) was 0.2-2.1 %. LOD and LOQ were 18 and 50 ng/band, respectively.

J. of Fujian Univ. of TCM 21(4), 38-40 (2011). TLC of Radix Serratulae Chinensis extracts on silica gel with chloroform - methanol 4:1. Detection under UV 254 nm. Identification of ecdysterone by comparison with the standard (hRf 42). The method was suitable for simple and reproducible quality control of Radix Serratulae Chinensis.

PHCOGJ 2(8), 211-215 (2011). TLC finger-print profiling of 4 cinnamomum species, C. malabatrum, C. sulphuratum, C. tamala and C. zeylanicum has been reported. The hexane extracts of the plants were separated on silica gel with toluene - ethyl acetate 8:1. Densitometric evaluation at 254 nm. Detection by dipping in vanillin-sulfuric acid reagent followed by heating at 105 °C and evaluation at 620 nm. The hRf value of eugenol was 69. The content of eugenol were higher in C. zeylanicum than in the other species. The fingerprint profile showed some other bands.

Phytochem. Anal. 24, 47-52 (2013) TLC of 22 acylated phenolic compounds on silica gel with acetonitrile - water - chloroform - formic acid 12:3:2:1, followed by dipping into 0.2 % methanolic 2,2-diphenyl-1-picrylhdrazyl solution (DPPH radical reagent) for 5 s and kept in the dark for 30 min. Free radical scavenging activity of the acylated phenolic compounds was assessed by coupling with the image processing software ImageJ .

J. of China Pharm. 21 (10), 31-32 (2012). Chuanyushaoshang cream is a herbal TCM preparation for external application for protecting wounds, promoting wound healing, antibiosis, invigorating the circulation of blood, and relieving pain. For quality control, TLC of emodin, one of the key active constituents in the extracts of the preparations, on silica gel with petroleum ether (60-90 ºC) – ethyl acetate 7:3, detection at UV 366 nm.

J. Planar Chromatogr. 25, 415-419 (2012). HPTLC of apigenin (1), quercetin (2), hyperoside (3), vitexin (4) and vitexin-2”-O-rhamnoside (5) on silica gel with acetonitrile - methanol - water 1:1:2 + 1 drop formic acid. Quantitative determination by absorbance measurement at 254 nm. The hRf of (1) to (5) were 12, 20, 48, 53 and 59, respectively. Linearity was in the range of 400-1250 ng/zone for (1) and (2) and 800-2500 ng/zone for (3) to (5). Limits of detection and quantification were 100 and 310 ng/zone for (1), 200 and 630 ng/zone for (2) and (3) and 300 and 960 ng/zone for (4) and (5), respectively, The intermediate/inter-day/intra-day precision was below 2.2 % (n=3). Recovery for all (1) to (5) was between 97.1 and 100.2 %.

J. Planar Chromatogr. 25, 439-444 (2012). Bivariant multiple development (BMD)-TLC of chelidonine (1), chelerithrine (2) and berberine (3) in the herb and roots of Chelidonium majus on silica gel with toluene - ethyl acetate - methanol 1:1:1 for step I, ethyl acetate - formic acid - water 7:1:1 for step II and n-propanol - formic acid - water 21:2:2 for step III. Quantitative determination by absorbance measurement at 290 nm. Linearity was in the range of 200-1500 ng/zone for (1), 200-1000 ng/zone for (2) and 250-1500 ng/zone for (3). Limits of detection and quantification were 100 and 200 ng/zone for (1), 50 and 200 ng/zone for (2) and 50 and 250 ng/zone for (3), respectively. The intermediate/inter-day/intra-day precision was below 1.6 % (n=7). Recovery for (1) to (3) was in the range of 96-105 %.

J. Liq. Chromatogr. Relat. Technol. 35, 1388-1403 (2012). HPTLC fingerprint of phenolic acids and flavonoids in 23 different sage species on silica gel with different mobile phases. When compared with spectrophotometric and HPLC/DAD methods, the HPTLC approach was a sufficient alternative by quickly providing a valuable complementary fingerprint material. HPTLC of 1) free phenolic acids and phenolic acids fractions derived from acid and basic hydrolysis, with benzene – ethyl acetate – formic acid 6:3:1; 2) Flavonoid aglycons with toluene – ethyl acetate –- formic acid 12:6:1; 3) basic flavonoid glycosides and acidic flavonoid glycosides fractions with ethyl acetate – water – formic acid – acetic acid 100:26:11:11. Detection under UV light at 366 nm.