J. Planar Chromatogr. 33, 587-597 (2020). HPTLC of plumbagin in fresh roots of Plumbago zeylanica on silica gel with toluene - acetic acid 18:1. Quantitative determination by absorbance measurement at 272 nm. The hRF value for plumbagin was 44. Linearity was between 500 and 4000 ng/zone. Intermediate precision was below 2 % (n=3). The LOD and LOQ were 1031 and 3439 ng/zone, respectively. Average recovery was 99.4 %.

J. Planar Chromatogr. 33, 567-577 (2020). HPTLC of atropine (1), caffeic acid (2) and chlorogenic acid (3) in the root, stem and leaf samples of different populations in two cytotypes of Physochlaina praealta on silica gel with methanol - acetone - diethylamine 25:24:1 for (1) and toluene - ethyl acetate - methanol - formic acid 75:25:10:6 for (2) and (3). Detection by spraying with Dragendorff’s reagent for (1) and anisaldehyde for (2) and (3). Quantitative determination by absorbance measurement at 254 and 500 nm. The hRF values for (1) to (3) were 18, 72 and 77. Linearity was between 2 and 12 µg/zone for (1) to (3). Intermediate precision was below 2 % (n=3). The LOD and LOQ were 490 and 1490 ng/zone for (1), 170 and 530 ng/zone for (2) and 220 and 650 ng/zone for (3), respectively.

Planta Med. 84(18), 1348-1354 (2018). A subfraction (obtained through liquid-liquid partition and column chromatography) of the ethanolic extract of whole Vernonia cinerea plants (Asteraceae, subf. Cichorioideae) was further fractioned by reverse-phase SPE (solid-phase extraction) followed by preparative TLC on silica gel layer (eluent not given). For verification, zones were detected by spraying with anisaldehyde solution with 10 % sulfuric acid, followed by heating at 100 °C. Further purification by reverse-phase HPLC allowed the isolation of 6 hirsutinolide-type sesquiterpenoids (all with a oxacyclonane forming an ether bridge), including vernolides A and B.

Planta Med. 84(18), 1340-1347 (2018). Preparative TLC on silica gel for the final purification of 15 phenylethyl-chromenone derivatives (including 3 with epoxide functions), 4 sesquiterpenoids (neopetasane, dehydrokaranone, dioxoselinene, ligudicin C) and a steroid (ergostatetraenone), all isolated through multi-step column chromatography from the ethyl-acetate fraction of a methanolic extract of Aquilaria sinensis resinous wood (Thymelaeaceae). For each compound, mobile phase, RF value and yield are given.

Quim. Nova. 43, 878-883 (2020). HPTLC of caryophyllene (1) and the diterpenic acids copalic (2), hydroxy-copalic (3), acetoxycopalic (4), agathic (5), and hardwickiic (6) in oilresin of Copaifera on silica gel with hexane - ethyl acetate 7:3. Detection by spraying with ethanol in 10 % sulfuric acid, followed by heating for 5 min. Qualitative analysis at 366 nm. The hRF values for (1) to (6) were 80, 50, 8, 28, 16 and 40, respectively.

Phytochem. Anal. 31, 711-721 (2020). HPTLC of 13 aporphine and four benzylisoquinoline-type alkaloids in the twigs of Ocotea spixiana on silica gel with propanol - water - ammonium hydroxide 18:2:1. Detection by desorption electrospray ionisation mass spectrometry (DESI-MS).

Phytochem. Anal. 31, 801-808 (2020). HPTLC of agarwood on silica gel with chloroform - methanol - distilled water 10:7:5. Detection by absorbance measurement at 254 nm. Agarotetrol was used as reference substance. Qualitative results were in agreement with the liquid extraction surface analysis mass spectrometry method.

J. Chinese Trad. Patent Med. 41 (3), 516-521 (2019). Yiqi Tongmai Capsule is a herbal TCM for activating blood circulation and regulating veins, as to relieve dizziness, chest tightness, chest pain caused by blood stasis. HPTLC for quality control on silica gel (1) for Ligusticum chuanxiong hort, with n-hexane - ethyl acetate 3:1 after presaturation of plates for 20 min, detection under UV 366 nm; (2) for Radix paeoniae rubra, with chloroform - ethyl acetate - methanol - formic acid 80:10:40:1 after presaturation of plates for 20 min, detection by spraying with 5 % vanilline in sulfuric acid - ethanol 1:4 and heating at 105 ˚C until the zones are detected in daylight, comparison with the standard  paeoniflorin; (3) for Panacis Quinquefolii Radix and Panaxnotoginseng (Burk.) F.H.Chen with trichloroform - anhydrous ethanol - water 10:10:1 after presaturation of plates for 15 min, detection by spraying with 10 % solution of sulfuric acid - ethanol 1:4 and heating at 105 ˚C until the zones are detected in daylight and under UV 366 nm, identification by comparison with the standards notoginsenoside R1, ginsenoside Rg1, Re, Rb1 and pseudoginsenoside F11.