International Journal of PharmTech Research 2(2), 1427-1430 (2010). Ashokarista formulations contain ashoka (Saraca indica) as the main ingredient. Its markers are catechin, (+)catechole, and (-)epicatechin. TLC of extracts and (+)catechin on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:4:0.1. Quantitative determination by densitometry in absorbance mode at 278 nm. For identification of the stem-bark of Saraca indica the fingerprint is evaluated after detection with anisaldehyde-sulphuric acid. The hRf value of (+)catechin was 54.

J. Liq. Chromatogr. Relat. Technol. 34, 579-590 (2011). HPTLC fingerprint of andrographolide (1), kutkoside (2), picroside-I (3), phyllanthin (4), and hypophyllanthin (5) in pharmaceutical formulations containing Andrographis paniculata, Phyllanthus amarus and Picrorhiza kurroa extracts, on silica gel with toluene - ethyl acetate - methanol - formic acid 8:7:2:1. Quantitative determination by absorbance measurement at 254 nm. The hRf values of (1) - (5) were 58, 13, 21, 73 and 82, respectively. The repeatability (%RSD) of sample application and measurement of peak areas was 0.1 and 0.3 % for (1), 0.04 and 0.14 % for (2), 0.13 and 0.11 % for (3), 0.08 and 0.07 % for (4), and 0.16 and 0.12 % for (5), respectively. Linearity was between 1000-3000 ng/zone for (1), 350-1050 ng/zone for (2) and (3), 175-525 ng/zone for (4), and 50-150 ng/zone for (5). The intermediate/inter-day/intra-day precisions (n = 7) were 0.2 % for (1-3), 0.1 % for (4), and 0.3 % for (5) .

Acta Chromatographica 22 (4), 549-567 (2010). HPTLC of risperidone on silica gel with methanol - ethyl acetate 4:1. The hRf value of risperidone was 34. Quantitative evaluation by absorbance measurement at 285 nm. The linearity was in the range of 100-600 ng/band (r=0.9996), the LOD was 22 ng/band and the LOQ was 68 ng/band. The method was suitable for selective analysis of risperidone and was successfully used for estimation of the equilibrium solubility of risperidone, and for quantification of risperidone as the bulk drug in a commercially available preparation, in in-house developed mucoadhesive microemulsion formulations, and in solution.

J. of Chromatogr. A 1245, 183-189 (2012). Anthocyanins are major flower pigments that can be affected by copigments, colorless compounds that can modify anthocyanin coloration. Application of TLC to separate and analyze anthocyanins and copigments. By slantingly cross loading samples compounds are symmetrically developed in various angles from the upper origin to individual Rf values and cross each other. Detection of copigments as color change on the developed line of anthocyanin. Demonstration by using pink sweet pea (Lathyrus odoratus L.) petals showing a more intense zone and a paler zone on the anthocyanin line. The zones were identified as kaempferol 3-rhamnoside and 2-cyanoethyl-isoxazolin-5-one. Kaempferol 3-rhamnoside is a flavonoid with general copigment effect of more intense and bluer coloration change, whereas the structure 2-cyanoethyl-isoxazolin-5-one is not a conventional copigment, it had a novel effect to change anthocyanin coloration paler while maintaining color tone.

Chinese J. of Subtropical Plant Sci. 41 (2), 13-18 (2012). Compound Prescription Cortex Phellodendri Chinensis fluid is a herbal TCM preparation, having remarkable curative effect to osteomyelitis, swelling and ulcer on the body surface and traumatic infection. For quality control, TLC on silica gel 1) for Cortex Phellodendri Chinensis with n-butanol – glacial acetic acid – water 9:2:1, detection by viewing at UV 366 nm, identification by fingerprint comparison in parallel with the standard drug and berberine chloride standard submitted to the same procedure; 2) for Flos Lonicerae and Fructus Forsythiae with the upper phase of ethyl acetate – formic acid – water 14:5:5, detection by viewing at UV 366 nm, identification by fingerprint comparison in parallel with the standard drug and chlorogenic acid standard submitted to the same procedure.

J. of Qilu Med. & Pharm. 31 (2), 75-78 (2012). Pudilan Xiaojan oral liquid is a herbal TCM preparation effective in clearing heat and removing toxicity, because of its antiinflammatory activity it is used to cure swelling caused by furuncles, pharyngitis, lymphadenitis and tonsillitis. Dandelion is the key component drug of the preparation. Analysis of the components of dandelion in the preparation by TLC on silica gel with chloroform – methanol – formic acid 18:2:1, detection under UV 366 nm.

J. of China Pharm. 19 (22), 41-43 (2010). Yanreqing capsules are a herbal TCM preparation specially effective for heat clearing and detoxifying, and prescribed clinically to cure bronchitis, pneumonia, acute tonsillitis, urinary system infections and biliary tract infections. TLC of the extracts of the medicine on silica gel 1) for Baical Skullcap, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 2 % ferric chloride in ethanol and viewing in daylight; 2) for Cape jasmine, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 110 °C until the zones are visible.

Brazilian Journal of Pharmacognosy 22, 1355-1361 (2012). HPTLC of piperine (1) and piperlongumine (2) on silica gel with toluene - ethyl acetate 3:2 as mobile phase. Quantitative determination by absorbance measurement at 342 and 325 nm for (1) and (2), respectively. The hRf of compounds (1) and (2) were 51 and 74, respectively. Linearity was in the range of 20-100 ng/zone for (1) and 30-150 ng/zone for (2). Limits of detection and quantification were 7 and 20 ng/zone for (1) and 10 and 30 ng/zone for (2), respectively. Intermediate/intra-day/inter-day precision was below 1.1 % (n=6). Recovery for both (1) and (2) was between 93.7 and 96.7 %.