Planta Med. 71, 673-679 (2005). Analytical and preparative TLC of 18-(beta-D-glucopyranosyloxy)-28-oxoolean-12-en-3beta-yl 3-O-(beta-D-glucopyranosyl)-beta-D-glucopyranosiduronic acid methyl ester, achyranthoside C dimethyl ester, achyranthoside C butyl dimethyl ester, achyranthoside E dimethyl ester, achyranthoside E butyl methyl ester (and 10 known compounds) on silica gel and RP18 with chloroform - methanol - water 8:5:2 or methanol - water 1:1, respectively. Detection under UV light at 254 nm or by spraying with cerium sulfate - 10 % sulfuric acid 1:99.

Planta Med. 71, 680-682 (2005). Analytical and preparative TLC of 5-hydroxy-2-methoxyxanthone, 2-hydroxy-3-methoxyxanthone, trans-kielcarin, 4-hydroxy-3-methoxyphenyl ferulate, and 3beta-O-caffeoylbetulinic acid on silica gel with chloroform - petroleum ether - formic acid 950:50:1, chloroform - acetone - formic acid 950:50:1, 900:100:1, and 850:150:1. Detection under UV light at 254 nm.

using high-performance thin-layer chromatography. J. AOAC Int. 89, 1467-1474 (2006). HPTLC of eugenol, luteolin, ursolic acid, and oleanolic acid on silica gel in a twin trough chamber with toluene - ethyl acetate - formic acid 35:15:1 at 25 °C and 40 % relative humidity. Quantitation in absorbance mode at 280 nm for eugenol, at 350 nm for luteolin, and at 530 nm for ursolic acid and oleanolic acid after derivatization with anisaldehyde-sulfuric acid reagent and heating at 105°C. The methods were validated for precision, repeatability, and accuracy.

Innovative Food Science and Emerging Technologies 1, 239-243 (2001). HPTLC of commercial herbal spirits (alcoholic or hydroalcoholic solutions of volatile substances with flavoring or medicinal properties) and one red wine on silica gel with toluene – ethyl formate – formic acid 79:20:1. Antioxidative components were detected by dipping for 30 s in a soybean oil solution (3 % in n-hexane, previously treated with active carbon). Quantitative determination in UV light at 254 nm after different times of UV-exposure (30 min – 20 h). The antioxidant activity could be evaluated from the fluorescence-persisting time of the respective spots and was correlated with linoleic acid oxidation and DPPH-titration methods. Although the nature of the active herbal antioxidants remains to be established, phenolic compounds seem to be key candidates.

cineraria (DC.) from middle Europe. Pharmazie 61, 559-561 (2006). Preparative TLC of seven pyrrolizidine alkaloids (senecionine, seneciphylline, integerrimine, jacobine, jacoline, jaconine, jacobine-acetate) on silica gel with dichloromethane - methanol - 25 % ammonia 75:24:1. Detection under UV light.

Pharmazie 60, 956-957 (2005). TLC of caffeic acid, patuletin, and patulitrin on silica gel by two fold development with benzene - ethanol - acetone 7:2:1; and ethyl acetate - iso-propanol - n-butanol - acetic acid - water 50:30:17.5:17.5:15 for sugars. Detection under UV 254 and 366 nm and by spraying with natural products reagent (for flavonoids) and p-anisidine followed by heating for 5 min (for sugars).

J. Planar Chromatogr. 20, 443-446 (2007). TLC of caffeoylmalic acid, forsythoside, and verbascoside on silica gel in an unsaturated chamber with formic acid - acetic acid - water - ethyl acetate 15:15:36:134. Detection by dipping into a 1 % methanolic solution of natural products reagent and heating for 10 min at 40 °C. The dried plates were subsequently dipped into a 5 % methanolic solution of polyethyleneglycol 400 and then heated as before. Quantitation by densitometry at 395 nm ??? in fluorescence mode.

59th Indian Pharmaceutical congress F-220, 442, (2007). HPTLC of 6-gingerol in herbal extracts on silica gel with n-hexane - ethyl acetate - ammonia 14:5:1 in a chamber saturated for 45 min. Densitometric evaluation at 254nm. The hRf value of 6-gingerol was 52. The method was linear in the range of 100 - 1200 ng/zone.