Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
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Journal of Chromatography B, 1184, 122956 (2021). Test for acetyl- and butyrylcholinesterase (AChE and BChE) inhibition without development of piperin (standard inhibitor of AChE and BChE) and ethanol – water (3:2) extracts of Iranian plants, on HPTLC silica gel prewashed twice with methanol – water 3:2 and dried 60 min at 120°C. After sample application the plate was immersed (speed 3.5 cm/s, time 2 s) into enzyme solution (6.6 units/mL AChE or 3.3 units/mL BChE in TRIS buffer 0.05 M, with bovine serum albumin 0.1 %, pH 7.8), incubation 25 min at 37°C and immersion (speed 3.5 cm/s, time 1 s) into chromogenic substrate solution (α-naphthyl acetate 0.1 % and Fast Blue salt B 0.2 % in ethanol – water, 1:2). Seven mobile phases were tested for the active samples. Best separation was obtained with toluene – ethyl acetate – formic acid – water 4:16:3:2 and with toluene – ethyl acetate – methanol 6:3:1. Before enzymatic assay, plates developed with acidic mobile phases were neutralized by spraying 3 mL citrate phosphate buffer (Na2HPO4 8 %, citric acid q.s. ad pH 7.5) followed by 10 min of automatic drying. Enzymatic assay was performed using a piezoelectric spraying device: a) pre-wetting by spraying 1 mL TRIS buffer (0.05 M, pH 7.8); b) spraying 3 mL of the enzyme solution; c) incubation 25 min in a humid box at 37°C; d) spraying 0.5 mL substrate solution; e) 5 min drying at room temperature, and then 10 min of automatic drying. By spraying, zone shift and zone diffusion, which occurred with plate immersion, were avoided. For development control, derivatization was done by piezoelectrically spraying 4 mL of sulfuric anisaldehyde reagent (anisaldehyde – sulfuric acid – acetic acid – methanol, 1:10:20:170), followed by heating 3 min at 110°C. For identification of zones of interest, direct elution with methanol from underivatized HPTLC plates through a TLC-MS interface directly to a MS. Identified zones were 3-O-acetyl-β-boswellic acid (triterpenoid) from Boswellia carteri gum-resin (Burseraceae), pimpinellin and psoralen (furocoumarins) from Heracleum persicum flowers (Apiaceae), oleuropein (seco-iridoid) from Olea europaea leaves (Oleaceae), harmine, harmaline, vasicine, deoxyvasine (alkaloids) from Peganum harmala seeds (Zygophyllaceae), costic acid (sesquiterpene) from Nardostachys jatamansi hypocotyl (Valerianaceae), elaidic, linoleic, palmitic, palmitoleic acids (fatty acids) from Pistacia atlantica fruits (Anacardiaceae).
J. Planar Chromatogr. 34, 353-359 (2021). HPTLC of alkaloids in the leaves of Solanum retroflexum on silica gel with chloroform - ethyl acetate - methanol 9:8:3. Detection under UV light at 365 nm. Further analysis of extracted zones by ultra-high performance liquid chromatography-quadrupole time-of-flight hyphenated to mass spectrometry (UHPLC‒QTOF‒MS).
J. Planar Chromatogr. 34, 329-336 (2021). HPTLC of piperine in the leaves of Piper trichostachyon on silica gel with n-hexane - ethyl acetate 1:3. Quantitative determination by absorbance measurement at 342 nm. The hRF value for piperine was 50. Linearity was between 100 and 700 μg/μL. LOD was 100 μg/μL. Intermediate precisions were below 3 %. Average recovery was 105.8 %.
J. Planar Chromatogr. 34, 157-163 (2021). HPTLC of berberine in the stem of Tinospora cordifolia on silica gel with n-hexane - ethyl acetate - methanol - formic acid 8:8:4:1. Quantitative determination by absorbance measurement at 360 nm. The hRF value for berberine was 48. Linearity was between 30 and 100 ng/zone. Intermediate precision was below 2 % (n=6). The LOD and LOQ were 10 and 30 ng/zone. Recovery was between 100.7 and 104.0 %.
J. Planar Chromatogr. 34, 147-155 (2021). HPTLC of berberine (1), berbamine (2), palmatine (3), magnoflorine (4) and jatrorrhizine (5) on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) to (5) were 47, 12, 38, 14 and 40. Linearity was between 1000 and 6000 ng/zone for (1) and (2), 200 and 700 ng/zone for (3), 500 and 2000 ng/zone for (4) and 300 and 1800 ng/zone for (5). Intermediate precision was below 2 % (n=3). The LOD and LOQ were 323 and 979 ng for (1), 227 and 688 ng for (2), 56 and 169 ng for (3), 89 and 289 ng for (4) and 87 and 289 ng for (5). Average recovery was 99.4 % for (1), 100.0 % for (2), 98.6 % for (3), 99.4 % for (4) and 98.9 % for (5).
J. Planar Chromatogr. 34, 31-38 (2021). HPTLC of reserpine (1), scopoletine (2), piperine (3), bacoside A (4) and lupeol (5) in a polyherbal formulation (containing Rauwolfia serpentina, Nardostachys jatamansi, Convolvulus pluricaulis, Bacopa monnieri, Piper longum) on silica gel with benzene - ethyl acetate - glacial acetic acid - n-butanol 57:30:10:3 for (1), (2) and (3), n-butanol - glacial acetic acid - water 18:3:4 for (4) and benzene - ethyl acetate 9:1 for (5). Detection by spraying with 5 % methanolic sulfuric acid solution, followed by heating at 80 °C for 20 min. Quantitative determination by absorbance measurement at 254 nm for (1) to (3), 598 nm for (4) and 580 nm for (5). The hRF values for (1) to (5) were 17, 53, 73, 34 and 48, respectively. Linearity was between 1 and 2 µg/zone for (1), 2.5 and 4.5 µg/zone for (2), 10 and 50 µg/zone for (3), 12 and 24 µg/zone for (4) and 1 and 5 µg/zone for (5). Intermediate precision was below 2 % (n=6). The LOD and LOQ were 6 and 20 ng/zone for (1), 13 and 38 ng/zone for (2), 11 and 33 ng/zone for (3), 377 and 1143 ng/zone for (4) and 324 and 982 ng/zone for (5), respectively. Average recovery was 98.1 %for (1), 99.9 % for (2), 99.9 for (3), 99.7 % for (4) and 99.5 % for (5).
Sci. World J. 2020, 7367836 (2020). TLC of ethyl acetate fractions of hydro ethanolic extracts obtained from Cassia siamea leaves (Caesalpiniaceae) on silica gel with chloroform – ethanol 17:3, as well as cassiarin A (an isoquinoline alkaloid, hRF 34) as standard. Visualization under UV light. Densitometry scanning at 368 nm for cassiarin A. Linearity was in the range of 400–1400 ng; intra-day precision was below 4 %; LOD and LOQ values were 3 and 8 ng, respectively; recovery rates were 102.8–120.1 %. The cassiarin A concentrations in the samples (given as w/v, the volumes being those of the first undried hydro-ethanolic extracts) varied depending on the origin (ground and climate) of the leaves.
3 Biotech 10(8), 344 (2020). Rauvolfia serpentina (Apocynaceae) was cultivated in vitro as leaf-derived callus and as plantlet cultures obtained from tissues of nodal explants, without or with cadmium chloride as elicitor of alkaloid production. TLC of methanol – ammonia 10:1 extracts of callus and plantlet organs (leaves, stems and roots) on silica gel, along with indole alkaloids reserpine and ajmalicine in different concentrations. Development with chloroform – toluene – ethyl acetate – diethyl amine 7:7:4:1. Detection under UV light and by densitometry scanning in absorbance mode at 240 nm and 280 nm. The highest alkaloid yields were obtained for reserpine (hRF 15) in roots (191µg/g) and for ajmalicine (hRF 45) in callus (131µg/ml) when culture had been done with elicitor 0.15 mM for 6 days and 4 days, respectively (at 0.20 mM, an inhibiting effect was observed).