Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
- Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.
J. Planar Chromatogr. 32, 243-249 (2019). HPTLC of stigmasterol (1) and cinnamic acid (2) in Pluchea dioscoridis on silica gel with chloroform - methanol - acetic acid 93:5:2. Quantitative determination of (1) by absorbance measurement at 254 nm. Compound (2) was detected by spraying with p-anisaldehyde and quantified under UV light at 513 nm. The hRF values for (1) and (2) were 57 and 19, respectivley. Linearity was between 200 and 1400 ng/zone for (1) and (2). The intermediate precision was below 2 % (n=6). The LOD and LOQ were 39 and 117 ng for (1) and 43 and 129 ng for (2), respectively. Recovery rate was between 98.8 and 99.4 % for (1) and 98.4 and 99.0 % for (2).
J. Sep. Sci. 30, 2786-2793 (2007). HPTLC of unsaponifiable constituents of rice bran oil on silica gel in two stage separation: First separation with benzene – chloroform 12:1 for sterols, oryzanols, and tocols. Quantitative determination by absorbance measurement at 206 nm for sterols (1), 325 nm for oryzanols (2), and 297 nm for tocols (3). Second separation with petroleum ether – diethyl ether 50:1 for steryl esters (4), wax (5), and squalene (6). Detection by dipping in 5 % methanolic sulphuric acid followed by heating at 110 °C for 1 hour. Quantitative determination by absorbance measurement at 439 nm. The hRf values were 12 for (1), 21 for (2), 39 for (3), 36 for (4), 46 for (5), and 74 for (6). Linearity was between 150 and 1200 ng/zone for the first separation and between 400 and 1200 ng/zone the second separation. The limits of detection and quantification were 6 and 20 ng/zone for (1), 1 and 4 ng/zone for (2), 11 and 38 ng/zone for (3), 22 and 73 ng/zone for (4), 19 and 65 ng/zone for (5), and 3 and 10 ng/zone for (6), respectively. Intra-assay precision was between 0.52 and 1.94 % and inter-assay precision was between 0.87 and 2.27 %. Recoveries ranged from 93.5 to 101.9 %.
?-amyrin and ?-sitosterol by applying a validated high-performance thin-layer chromatography method in the aerial parts of Tinospora cordifolia and Calotropis gigantia
J. Planar Chromatogr. 30, 175-180 (2017). HPTLC of β-amyrin (1) and β-sitosterol (2) in the aerial parts of Tinospora cordifolia and Calotropis gigantia on silica gel with n-hexane – ethyl acetate 3:1. Detection by spraying with p-anisaldehyde reagent. Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) and (2) were 39 and 26, respectively. Linearity was between 100 and 1400 ng/zone for (1) and (2). LOD and LOQ were 18 and 55 ng/zone for (1) and 30 and 91 ng/zone for (2). The intermediate precision was <2 % (n=6). Recovery rate ranged from 98.4 to 99.3 % for (1) and 98.3 to 99.9 % for (2).
Lipids 17, 477-482 (1982). Preparative TLC of microbially produced metabolites of lithocholic acid and lithocholic acid-3 alpha-sulfate on silica with dichloromethane-methanol 95:5. Detection by spraying with anisaldehyde, followed by heating. The following metabolites were isolated: isolithocholic acid, 5 beta-cholanic acid, 3-keto-lithocholic acid, 5 alpha -cholestone and delta 3-cholenic acid.
J. Liquid Chrom. 10, 1277-1290 (1987). TLC on silica, Ag+ impregnated silica with e.g. isopropyl ether - acetic acid 96:4, then with petrol ether - acetic acid 90:10:1. Also chromatography on RP-18 silica. Derivatization with phosphomolybdic acid reagent, for quantification with copper acetate - H3PO4 reagent. Quantification by densitometry.
Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 49-55 (1991). Separation of ceramides and cholesterol on silica, using a four-step 2-D developing technique: first direction with chloroform - acetone - methanol 90:5:5 over 1 cm, second direction with the same solvent over 8.5 cm, then in the first direction again with chloroform - acetone - methanol - NH3 60:30:8:2, and finally with chloroform - acetone - methanol - acetic acid - water 78:16:4:2:1 in the second direction. Derivatization by dipping in phosphoric acid - acetic acid - sulfuric acid - water 10:10:1:180 followed by heating at 200 °C for 10 min. Method for routine analysis of ceramides.
Chinese J. Herb Med. (Zhongcaoyao) 24, 128-130 (1993). TLC of palmityl-ß-sitosterol, lupenone, 24-methylene-cycloartenol, and ß-sitosterol on silica with chloroform - benzene 9:1. Detection by spraying with 5% phosphomolybdic acid. Quantification by densitometry (absorbance) at 625 nm.
J. Planar Chromatogr. 13, 437-442 (2000). TLC of cholesterol and oxysterols (7-ketosterol, 25-hydroxycholesterol, 7b-hydroxycholesterol, cholesterol-5a,6a-epoxide, cholestane-3b,5a,6b-triol) on silica gel with chloroform-acetone 9:1. After spraying with Liebermann-Burchard reagent the spots were quantified by densitometry in reflectance mode at 360 nm. Also TLC of silylated oxysterols on silanized silica gel with n-hexane - ether - methanol 38:2:1. Simple, sensitive, and reproducible method.