Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
  • Keyword register: select an initial character and browse associated keywords
  • Search by CBS edition: Select a CBS edition and find all related publications

Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. The saved items can be printed to PDF using the print function of your web browser.

      Quantification of two biomarker compounds by a validated High-Performance Thin-Layer Chromatographic method from different extracts of Pluchea dioscoridis growing in Saudi Arabia
      H. AL-YOUSEF*, Y. ALHOWIRINY, N. SIDDIQUI, P. ALAM, W. HASSAN, M. AMINA (*Pharmacognosy Department, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia, halyousef@ksu.edu.sa)

      J. Planar Chromatogr. 32, 243-249 (2019). HPTLC of stigmasterol (1) and cinnamic acid (2) in Pluchea dioscoridis on silica gel with chloroform - methanol - acetic acid 93:5:2. Quantitative determination of (1) by absorbance measurement at 254 nm. Compound (2) was detected by spraying with p-anisaldehyde and quantified under UV light at 513 nm. The hRF values for (1) and (2) were 57 and 19, respectivley. Linearity was between 200 and 1400 ng/zone for (1) and (2). The intermediate precision was below 2 % (n=6). The LOD and LOQ were 39 and 117 ng for (1) and 43 and 129 ng for (2), respectively. Recovery rate was between 98.8 and 99.4 % for (1) and 98.4 and 99.0 % for (2).

      Classification: 13c, 11a
      100 034
      A new method for simultaneous estimation of unsaponifiable constituents of rice bran oil using HPTLC
      L. AFINISHA, D. SOBAN, A. SUNDARESAN, C. ARUMUGHAN* (*National Institute for Interdisciplinary Science and Technology, Kerala, India, carumughan@yahoo.com)

      J. Sep. Sci. 30, 2786-2793 (2007). HPTLC of unsaponifiable constituents of rice bran oil on silica gel in two stage separation First separation with benzene – chloroform 121 for sterols, oryzanols, and tocols. Quantitative determination by absorbance measurement at 206 nm for sterols (1), 325 nm for oryzanols (2), and 297 nm for tocols (3). Second separation with petroleum ether – diethyl ether 501 for steryl esters (4), wax (5), and squalene (6). Detection by dipping in 5 % methanolic sulphuric acid followed by heating at 110 °C for 1 hour. Quantitative determination by absorbance measurement at 439 nm. The hRf values were 12 for (1), 21 for (2), 39 for (3), 36 for (4), 46 for (5), and 74 for (6). Linearity was between 150 and 1200 ng/zone for the first separation and between 400 and 1200 ng/zone the second separation. The limits of detection and quantification were 6 and 20 ng/zone for (1), 1 and 4 ng/zone for (2), 11 and 38 ng/zone for (3), 22 and 73 ng/zone for (4), 19 and 65 ng/zone for (5), and 3 and 10 ng/zone for (6), respectively. Intra-assay precision was between 0.52 and 1.94 % and inter-assay precision was between 0.87 and 2.27 %. Recoveries ranged from 93.5 to 101.9 %.

      Classification: 13c
      119 054
      Concurrent analysis of the biologically active markers
      ?-amyrin and ?-sitosterol by applying a validated high-performance thin-layer chromatography method in the aerial parts of Tinospora cordifolia and Calotropis gigantia
      M. ALAJMI, A. HUSSAIN, P. ALAM* (*Department of Pharmacognosy, College
      of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia, alamperwez007@gmail.com)

      J. Planar Chromatogr. 30, 175-180 (2017). HPTLC of ?-amyrin (1) and ?-sitosterol (2) in the aerial parts of Tinospora cordifolia and Calotropis gigantia on silica gel with n-hexane – ethyl acetate 31. Detection by spraying with p-anisaldehyde reagent. Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) and (2) were 39 and 26, respectively. Linearity was between 100 and 1400 ng/zone for (1) and (2). LOD and LOQ were 18 and 55 ng/zone for (1) and 30 and 91 ng/zone for (2). The intermediate precision was <2 % (n=6). Recovery rate ranged from 98.4 to 99.3 % for (1) and 98.3 to 99.9 % for (2).

      Keywords:
      Classification: 13c
      55 038
      The metabolism of lithocholic acid and lithocholic acid-3 alpha sulfate by human fecal bacteria
      S. BORRIELLO, R. OWEN

      Lipids 17, 477-482 (1982). Preparative TLC of microbially produced metabolites of lithocholic acid and lithocholic acid-3 alpha-sulfate on silica with dichloromethane-methanol 955. Detection by spraying with anisaldehyde, followed by heating. The following metabolites were isolated isolithocholic acid, 5 beta-cholanic acid, 3-keto-lithocholic acid, 5 alpha -cholestone and delta 3-cholenic acid.

      Keywords:
      Classification: 13c, 13d, 11c, 32b, 32d
      61 094
      Determination of cholesterol in egg yolk by high performance TLC with densitometry
      K. MORRIS, J. SHERMA, B. FRIED, (Dept. Chem., Lafayette College, Easton, Pennsylvania 18042, USA)

      J. Liquid Chrom. 10, 1277-1290 (1987). TLC on silica, Ag+ impregnated silica with e.g. isopropyl ether - acetic acid 964, then with petrol ether - acetic acid 90101. Also chromatography on RP-18 silica. Derivatization with phosphomolybdic acid reagent, for quantification with copper acetate - H3PO4 reagent. Quantification by densitometry.

      Keywords:
      Classification: 13c
      67 071
      Separation of ceramides from cultured human skin by means of two dimensional HPTLC
      J. BROD, P. JUSTINE, F. BERNAUD, E. BAVELIER, (Lab. de. Recherche Fondamentale de L’Oreal, 93600 Aulnay-sous-Bois, France)

      Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 49-55 (1991). Separation of ceramides and cholesterol on silica, using a four-step 2-D developing technique first direction with chloroform - acetone - methanol 9055 over 1 cm, second direction with the same solvent over 8.5 cm, then in the first direction again with chloroform - acetone - methanol - NH3 603082, and finally with chloroform - acetone - methanol - acetic acid - water 7816421 in the second direction. Derivatization by dipping in phosphoric acid - acetic acid - sulfuric acid - water 10101180 followed by heating at 200 °C for 10 min. Method for routine analysis of ceramides.

      Classification: 11c, 13c
      73 050
      (Analysis of the fat-soluble constituents in Chinese drugs shashen by thin-layer chromatography
      P. TU (Tu Pengfei), G. XU (Xu Guojun), L. XU (Xu Luoshan), R. JIN (Jin Rongluan), (China Pharm. Univ., Nanjing 210009, P.R. China)

      ) (Chinese). Chinese J. Herb Med. (Zhongcaoyao) 24, 128-130 (1993). TLC of palmityl-ß-sitosterol, lupenone, 24-methylene-cycloartenol, and ß-sitosterol on silica with chloroform - benzene 91. Detection by spraying with 5% phosphomolybdic acid. Quantification by densitometry (absorbance) at 625 nm.

      Classification: 13c
      87 041
      Analysis of oxygenated cholesterol derivatives by planar chromatography
      B. JANOZKA*, T. WILKOSZYNSKI, K. TYRPIEN, C. DOBOSZ, P. BODZEK. (*Silesian Med. Acad., Fac. of Med., Dept. of Chem., H. Jordana 19, 41-808 Zabrze, Poland)

      J. Planar Chromatogr. 13, 437-442 (2000). TLC of cholesterol and oxysterols (7-ketosterol, 25-hydroxycholesterol, 7b-hydroxycholesterol, cholesterol-5a,6a-epoxide, cholestane-3b,5a,6b-triol) on silica gel with chloroform-acetone 91. After spraying with Liebermann-Burchard reagent the spots were quantified by densitometry in reflectance mode at 360 nm. Also TLC of silylated oxysterols on silanized silica gel with n-hexane - ether - methanol 3821. Simple, sensitive, and reproducible method.

      Keywords:
      Classification: 13c