Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      131 002
      Bioassay-guided fractionation leads to the detection of cholic acid generated by the rare Thalassomonas sp.
      F. PHEIFFER, Y. K.-H. SCHNEIDER, E. H. HANSEN, J. HAMMER ANDERSEN, J. ISAKSSON, T. BUSCHE, C. RÜCKERT, J. KALINOWSKI, L. van ZYL, Marla TRINDADE* (*Institute for Microbial Biotechnology and Metagenomics, Department of Biotechnology, University of the Western Cape, Bellville, Cape Town, South Africa;

      Marine Drugs 21(1), 2 (2023). Samples were methanol extracts of cultivated marine bacteria Thalassomonas actiniarum, T. viridans and T. haliotis (Colwelliaceae),  as well as cholesterol, cholic acid, and deoxycholic acid as standards. TLC on silica gel with n-hexane – ethyl acetate – methanol – acetic acid 20:20:5:2. After drying at room temperature, visualization by spraying with phosphomolybdic acid (10 % in ethanol) and heating with a heat-gun. For isolation of cholic acid (hRF 80), present in all samples, preparative TLC on silica gel with the same mobile phase, the corresponding band was scraped off with a surgical blade and extracted with methanol overnight. The isolated cholic acid was identified by LC-MS.

      Classification: 13c, 13d
      130 143
      Estimation of withaferin-A by HPLC and standardization of the Ashwagandhadi lehyam formulation
      A. K. MEENA*, P. REKHA, A. PERUMAL, M. GOKUL, K.N. SWATHI, R. ILAVARASAN (*Captain Srinivasa Murthy Regional Ayurveda Drug Development Institute, Central Council for Research in Ayurvedic Sciences, Arumbakkam, Chennai, India;

      Heliyon 7(2), e06116 (2021). Samples were a methanolic extract of a semi-solid ayurvedic conserve (ashwagandhadi lehyam) prepared with Withania somnifera roots (Solanaceae) and five other plants, as well as standards: withaferin A and withanolide A (= withaniol), two ergostane triterpene steroids with lactone cycle and epoxide. HPTLC on silica gel with toluene – ethyl acetate – formic acid 6:4:1. Visualization and densitometric scanning at UV 254 nm and 366 nm (deuterium lamps). Derivatization by immersion into vanillin – sulfuric acid reagent, followed by oven heating at 105 °C until optimal coloration. Documentation under white light and densitometry scanning at 540 nm (tungsten lamp). Both analytes (hRF 35 and 45 respectively) were shown at 254 nm and 540 nm (but not at 366 nm), in the standards and in the extract.

      Classification: 8b, 9, 13c, 15a, 32e
      130 146
      Development of a thin-layer chromatography bioautographic assay for neuraminidase inhibitors hyphenated with electrostatic field induced spray ionisation-mass spectrometry for identification of active Isatis indigotica root compounds
      Y. ZANG (Zang Yichao), Y. MIAO (Miao Yu), T. WU (Wu Tao)*, Z. CHENG (Cheng Zhihong)** (*Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China,; **Department of Natural Medicine, School of Pharmacy, Fudan University, Shanghai, China,

      J Chromatogr A 1638, 461597 (2021). Samples were Isatis tinctoria (= I. indigotica) root extracts (Brassicaceae) and their fractions. Standards were oseltamivir acid (OA), a neuraminidase (NA) inhibitor; pinoresinol (PR, a lignan), β-sitosterol (SS, a sterol), and dihydro-neoascorbigen (DHNA, an alkaloid). HPTLC / TLC on silica gel with (1) petroleum ether – ethyl acetate – acetic acid 48:8:1 for petroleum ether extracts and SS, or 30:40:1 for ethyl acetate extracts, or 10:30:1 for PR; (2) with toluene – ethyl acetate – methanol – formic acid 16:3:1:2 or 10:4:1:2 also for ethyl acetate extracts and DHNA; (3) with n-butanol – acetic acid – water 25:4:3 for butanol extracts. OA was applied but not developed. RP-18, polyamide, cellulose, alumina layers were tested, but the resolution was lower. Derivatization by spraying with sulfuric acid (10 % in ethanol). Enzymatic assay by immersion of the plates into neuraminidase solution (6 U/mL), followed by 1 h incubation at 37 °C and by immersion into chromogenic substrate solution (1.75 mM 5-bromo-4-chloro-3-indolyl-α-D-N-acetylneuraminic acid). After 5 min, NA inhibitors were seen as white zones on blue background. The experiment was previously improved for the following parameters: incubation times, substrate and enzyme concentrations, followed by statistical evaluation and calculations using Box-Behnken design. Quantification by absorbance measurement (detection wavelength 605 nm, reference wavelength 420 nm). In optimal conditions, OA had LOD 300 ng/zone. Zones of interest on underivatized plates were directly submitted to MS, using EFISI (electrostatic-field-induced spray ionisation), as follows. Chromatograms were immersed 1–3 s into dimethicone – n-hexane 1:1 to form a hydrophobic film, and dried 30 min at room temperature; on the analyte spot, a hydrophilic droplet was formed with 5 µL methanol – water 1:1, extracting the analyte from the layer; the analyte was further attracted through a capillary tube (3–4 cm long, made of non-deactivated fused silica) under a strong electrostatic field, into the in-let orifice of the triple-quadrupole ­– linear ion-trap MS (induction voltage 4 kV; capillary voltage 40 V; tube lens voltage 100 V; capillary temperature 200 °C). Full-scan spectra were recorded in m/z range 50 – 1000, helium was used for collision-induced dissociation. 11 active compounds were identified in the extract: SS, 6 alkaloids (including cycloanthranilylproline, DHNA, hydroxy-indirubin, isatindigodiphindoside, isatindinoline A and), 3 lignans (including PR and isolariciresinol), 1 fatty acid (trihydroxy-octadecenoic acid).

      Classification: 4e, 8a, 8b, 11a, 13c, 22
      130 046
      Development of synergy-based combination for learning and memory using in vitro, in vivo and TLC-MS-bioautographic studies
      M. KHAN, V. SRIVASTAVA, M. KABIR, M. SAMAL, A. INSAF, M. IBRAHIM, S. ZAHIRUDDIN, S. AHMAD* (*Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India,

      Front. Pharmacol. 12, 678611 (2021). HPTLC of withanolide S in Withania somnifera and myristicin in Myristica fragrans on silica gel with toluene - ethyl acetate - acetic acid 5:4:1. Detection of myristicin under UV 254 nm. Detection of withanolide S by spraying with 5 % anisaldehyde sulfuric acid, followed by visualization under UV 540 nm. Screening of anticholinesterase active metabolites by spraying with DTNB/ATCI reagent (1 mmol/L 5,5-dithiobis-(2- nitrobenzoic acid) and 1 mmol/L acetyl thiocholine iodide). 

      Classification: 13c
      130 058
      Standardization and validation of phytometabolites by UHPLC and high-performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul
      A. BALKRISHNA, M. TOMER, M. JOSHI, S. GUJRAL, R. MISHRA, J. SRIVASTAVA, A. VARSHNEY* (*Anurag Varshney, Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar 249 405, Uttarakhand, India,

      J. Sep. Sci. 45, 1616-1635 (2022). HPTLC of gallic acid (1), protocatechuic acid (2), vanillic acid (3), cinnamic acid (4), piperine (5), guggulsterone-E (6), and guggulsterone-Z (7) in Mahayograj Guggul on silica gel with toluene - ethyl acetate - formic acid 10:9:2 for (1) to (3) and toluene - acetone 9:1 for (4) to (7). Quantitative determination by absorbance measurement at 250 nm for (6) and (7), 280 nm for (1), (2), (3) and (4) and 343 nm for (5). The hRF values for (1) to (7) were 30, 41, 47, 15, 33, 41 and 45, respectively. Linearity was between 100 and 1000 µg/mL for (1), 5 and 60 µg/mL for (2), 10 and 80 µg/mL for (3), (4) and (7), 20 and 100 µg/mL for (5) and 40 and 120 µg/mL for (6). Inter-day and intra-day precisions were below 4 % (n=18). The LOD and LOQ were 4 and 14 µg/g for (1), 7 and 21 µg/g for (2), 24 and 72 µg/g for (3), 0.8 and 2.4 µg/g for (4), 12 and 35 µg/g for (5), 2 and 6 µg/g for (6) and 4 and 14 µg/g for (7), respectively. Recovery was between 86.6 and 102.0 % for (1) to (7).

      Classification: 8a, 13c
      130 080
      HPTLC determination of ergosterol in wheat and structure elucidation by NMR: Toward confirming method selectivity
      A. IBRAHIM, M. ORABY, A. KHORSHED* (*Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag 82524, Egypt,

      J. Food Compos. Anal. 114, 104763 (2022). HPTLC of ergosterol in wheat, on silica gel with ethyl acetate - petroleum ether 3:2. Quantitative determination by absorbance measurement at 282 nm. The hRF value for ergosterol was 45. Linearity was between 40 and 600 ng/zone. Inter-day and intra-day precisions were below 4 % (n=6). The LOD and LOQ were 11 and 36 ng/zone. Recovery was between 103.7 and 107.7 %. 

      Classification: 13c
      130 032
      Inhibition of intestinal lipid absorption by cyanobacterial strains in zebrafish larvae
      M. BELLVER, S. LEMOS da COSTA, B. ASTRAIN SANCHEZ, V. VASCONCELOS, R. URBATZKA* (*Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Matosinhos, Portugal;

      Marine Drugs 19(3), 161 (2021). Samples were a standard mix (tripalmitin, palmitic acid, cholesterol, phosphatidylcholine) and lipid-enriched extracts of zebrafish larvae (Danio rerio, Cyprinidae), that were anesthetized with tricaine after having being treated with 11 extracts of cyanobacteria strains and/or with a green fluorescent lipid analogue of fatty acids (BODIPY-C16, bore-dipyrromethene derivative). HPTLC on nano silica gel in 3 steps: 1) and 2) with chloroform – methanol – water 12:6:1 (twice up to 4 cm); 3) hexane – diethyl ether – acetic acid 160:40:3 (once up to 9 cm). Derivatization of lipids by spraying primuline solution (0.01 % in acetone – water, 3:2). Quantification based on fluorescence peak area intensity, was performed using image software on pictures taken through a green fluorescence imager. Triglycerides were decreased in the case of larvae treated with 2 extracts of Synechocystis strains (Merismopediaceae), but the levels of other lipid classes were not affected. No treatment significantly affected the incorporation of BODIPY-C16 into any of the lipid classes of the larvae.

      Classification: 11a, 11c, 13c, 32d
      130 116
      Agar‑free high‑performance thin‑layer chromatography–bioautography method for the qualitative estimation of α‑amylase inhibitor in Syzygium cumini seed extract and formulation
      S. BHUJBAL*, B. CHAWALE, M. KALE, R. BHUJBAL (*Department of Pharmacognosy, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Sant. Tukaram Nagar Pimpri, Pune, Maharashtra 411018, India,

      J. Planar Chromatogr. 35, 501-507 (2022). HPTLC of β-sitosterol the seeds of Syzygium cumini on silica gel with toluene - ethyl acetate - methanol - glacial acetic acid 6:3:2:1. Detection by dipping into anisaldehyde sulfuric acid solution for 3 s, followed by heating at 80-100 °C for 2-3 min. The assessment of α-amylase inhibitors was performed by dipping into enzyme solution (10 mg α-amylase enzyme in 20 mL of sodium acetate buffer and stored at 2–8 °C) for 2–3 seconds, followed by 90 min humidification in a desiccator and the dipping into a 1 % starch solution as a substrate and put in a humid environment for additional 20–30 min to allow the enzyme–substrate interaction to occur. After a 2–5-min time, the plate was washed or dipped in Gram’s iodine blue, which revealed anti-diabetic activity as blue stains on a white background. The hRF value for β-sitosterol was 87. Further analysis by high‑resolution mass spectrometry.

      Classification: 13c