Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
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      128 048
      Cholestasis impairs hepatic lipid storage via AMPK and CREB signaling in hepatitis B virus surface protein transgenic mice
      K. IRUNGBAM, M. RODERFELD, H. GLIMM, F. HEMPEL, F. SCHNEIDER, L. HEHR, D. GLEBE, Y. CHURIN, G. MORLOCK, I. YÜCE, Elke ROEB* (*Department of Gastroenterology, Justus Liebig University Giessen, Giessen, Germany; elke.roeb@innere.med.uni-giessen.de)

      Nature - Lab. Invest. 100, 1411–1424 (2020). Samples were chloroform – methanol 1:1 solutions of lipid standards and of liver tissue extracts from wild-type mice (1), from transgenic murine models of hepatic steatosis (2) (mice expressing HBs, hepatitis B virus surface protein), or of cholestasis (3) (mice totally knock-out for the gene of phospholipid translocator ABCB4, ATP-binding cassette subfamily B member 4), or of both (4) (hybrids of mice (2) and (3)). HPTLC on silica gel (preheated at 110°C for 15 min) with n-hexane – diethyl ether – acetic acid 20:5:1. (A) For qualitative analysis, visualization under white light after immersion into anisaldehyde 0.5 % (in sulfuric acid – acetic acid – methanol, 1:2:17), followed by heating at 110°C for 9 min. (B) Identification of lipids was confirmed by elution of the zones of interest with methanol from the HPTLC layer through a TLC-MS interface and a filter frit directly to a quadrupole-orbitrap MS (atmospheric pressure chemical ionization, full HR-MS scan in m/z range 100–1000). (C) For quantitative analysis, visualization at UV 366 nm after derivatization by immersion into primuline reagent (primuline 0.5 g/L in acetone – water 4:1); fluorescence was measured at UV 366 nm (mercury lamp, optical filter for wavelengths above 400 nm, scanning slit 6.0 mm × 0.2 mm, speed 20 mm/s). (A) and (B) allowed the separation and detection of cholesterol, cholesteryl oleate, methyl oleate, free fatty acids (FFA, expressed as oleic acid equivalents) and triacylglycerols (TAG, as triolein equivalents) in liver extracts. (C) showed that TAG was decreased and FFA increased in (3) and (4), compared to (1) and (2). Cholesterol and cholesteryl oleate had no significant changes between groups.

      Classification: 4e, 11a, 11c, 13c
      127 044
      Organ‑based chemo‑profiling of Echinops echinatus Roxb. using high‑performance thin‑layer chromatography (HPTLC) technique
      N. BHATT*, R. GUPTA, Y. BANSAL (*Department of Botany, Punjabi University, Patiala, Punjab, India, bhattnusrat91@gmail.com)

      J. Planar Chromatogr. 34, 173-181 (2021). HPTLC of caffeic acid (1), chlorogenic acid (2), quercetin (3), oleanolic acid (4), lupeol (5), betulinic acid (6), β-Sitosterol (7), campesterol (8) and ergosterol (9) in samples of Echinops echinatus on silica gel with toluene - ethyl acetate - formic acid - methanol 30:30:8:3 for (1) and (2), toluene - ethyl acetate - formic acid 5:4:1 for (3), toluene - methanol 9:1 for (4), petroleum ether - ethyl acetate - toluene 7:2:1 for (5) and (6), toluene - ethyl acetate 9:1 for (7) and toluene - methanol - formic acid for (8) and (9). Quantitative determination by absorbance measurement at 254 nm for (1) to (3), 540 nm for (4) to (6) and 530 nm for (7) to (9). The hRF values for (1) to (9) were 69, 80, 60, 30, 68, 55, 26, 67 and 75, respectively. Linearity was between 2 and 12 µg/mL for (1) to (9). Intermediate precision was below 2 % (n=6). The LOD and LOQ were 38 and 119 ng/zone for (1), 18 and 57 ng/zone for (2), 364 and 1100 ng/zone for (3), 11 and 32 ng/zone for (4), 40 and 123 ng/zone for (5), 15 and 46 ng/zone for (6), 8 and 23 ng/zone for (7), 52 and 159 ng/zone for (8) and 527 and 1598 ng/zone for (9), respectively. Recovery was between 95.7 and 99.6 % for (1) to (9). 

      Classification: 8a, 13c, 14
      127 003
      Lanostane triterpenes from Gloeophyllum odoratum and their anti-influenza effects
      Ulrike GRIENKE*, J. ZWIRCHMAYR, U. PEINTNER, E. URBAN, M. ZEHL, M. SCHMIDTKE, J. M. ROLLINGER (*Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna, Vienna, Austria; ulrike.grienke@univie.ac.at)

      Planta Med. 85(3), 195-202 (2019). The dichloromethane fraction of an ethanolic extract from Gloeophyllum odoratum sporocarp (Gloeophyllaceae, Basidiomycetes) was submitted to a multistep purification process (conventional, flash and supercritical fluid column chromatography). At each step, fractions were monitored on TLC silica gel with dichloromethane – methanol – water 40:4:1. Detection under white and UV light after derivatization with vanillin sulfuric acid 5 % in methanol and heating. Eight triterpenes were isolated for further identification: eburicodiol, gloeophyllins B and K, hydroxylanosterol, trametenolic acid B (all five from the lanostane type), gloeophyllins A and L (C‑nor-D-homoergosteroid type), and ergosterol peroxide (ergostane type).

      Classification: 13c, 15a, 32e
      123 027
      Quantification of two biomarker compounds by a validated High-Performance Thin-Layer Chromatographic method from different extracts of Pluchea dioscoridis growing in Saudi Arabia
      H. AL-YOUSEF*, Y. ALHOWIRINY, N. SIDDIQUI, P. ALAM, W. HASSAN, M. AMINA (*Pharmacognosy Department, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia, halyousef@ksu.edu.sa)

      J. Planar Chromatogr. 32, 243-249 (2019). HPTLC of stigmasterol (1) and cinnamic acid (2) in Pluchea dioscoridis on silica gel with chloroform - methanol - acetic acid 93:5:2. Quantitative determination of (1) by absorbance measurement at 254 nm. Compound (2) was detected by spraying with p-anisaldehyde and quantified under UV light at 513 nm. The hRF values for (1) and (2) were 57 and 19, respectivley. Linearity was between 200 and 1400 ng/zone for (1) and (2). The intermediate precision was below 2 % (n=6). The LOD and LOQ were 39 and 117 ng for (1) and 43 and 129 ng for (2), respectively. Recovery rate was between 98.8 and 99.4 % for (1) and 98.4 and 99.0 % for (2).

      Classification: 11a, 13c
      100 034
      A new method for simultaneous estimation of unsaponifiable constituents of rice bran oil using HPTLC
      L. AFINISHA, D. SOBAN, A. SUNDARESAN, C. ARUMUGHAN* (*National Institute for Interdisciplinary Science and Technology, Kerala, India, carumughan@yahoo.com)

      J. Sep. Sci. 30, 2786-2793 (2007). HPTLC of unsaponifiable constituents of rice bran oil on silica gel in two stage separation: First separation with benzene – chloroform 12:1 for sterols, oryzanols, and tocols. Quantitative determination by absorbance measurement at 206 nm for sterols (1), 325 nm for oryzanols (2), and 297 nm for tocols (3). Second separation with petroleum ether – diethyl ether 50:1 for steryl esters (4), wax (5), and squalene (6). Detection by dipping in 5 % methanolic sulphuric acid followed by heating at 110 °C for 1 hour. Quantitative determination by absorbance measurement at 439 nm. The hRf values were 12 for (1), 21 for (2), 39 for (3), 36 for (4), 46 for (5), and 74 for (6). Linearity was between 150 and 1200 ng/zone for the first separation and between 400 and 1200 ng/zone the second separation. The limits of detection and quantification were 6 and 20 ng/zone for (1), 1 and 4 ng/zone for (2), 11 and 38 ng/zone for (3), 22 and 73 ng/zone for (4), 19 and 65 ng/zone for (5), and 3 and 10 ng/zone for (6), respectively. Intra-assay precision was between 0.52 and 1.94 % and inter-assay precision was between 0.87 and 2.27 %. Recoveries ranged from 93.5 to 101.9 %.

      Classification: 13c
      119 054
      Concurrent analysis of the biologically active markers
      ?-amyrin and ?-sitosterol by applying a validated high-performance thin-layer chromatography method in the aerial parts of Tinospora cordifolia and Calotropis gigantia
      M. ALAJMI, A. HUSSAIN, P. ALAM* (*Department of Pharmacognosy, College
      of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia, alamperwez007@gmail.com)

      J. Planar Chromatogr. 30, 175-180 (2017). HPTLC of β-amyrin (1) and β-sitosterol (2) in the aerial parts of Tinospora cordifolia and Calotropis gigantia on silica gel with n-hexane – ethyl acetate 3:1. Detection by spraying with p-anisaldehyde reagent. Quantitative determination by absorbance measurement at 530 nm. The hRF values for (1) and (2) were 39 and 26, respectively. Linearity was between 100 and 1400 ng/zone for (1) and (2). LOD and LOQ were 18 and 55 ng/zone for (1) and 30 and 91 ng/zone for (2). The intermediate precision was <2 % (n=6). Recovery rate ranged from 98.4 to 99.3 % for (1) and 98.3 to 99.9 % for (2).

      Classification: 13c
      55 038
      The metabolism of lithocholic acid and lithocholic acid-3 alpha sulfate by human fecal bacteria
      S. BORRIELLO, R. OWEN

      Lipids 17, 477-482 (1982). Preparative TLC of microbially produced metabolites of lithocholic acid and lithocholic acid-3 alpha-sulfate on silica with dichloromethane-methanol 95:5. Detection by spraying with anisaldehyde, followed by heating. The following metabolites were isolated: isolithocholic acid, 5 beta-cholanic acid, 3-keto-lithocholic acid, 5 alpha -cholestone and delta 3-cholenic acid.

      Classification: 11c, 13c, 13d, 32b, 32d
      61 094
      Determination of cholesterol in egg yolk by high performance TLC with densitometry
      K. MORRIS, J. SHERMA, B. FRIED, (Dept. Chem., Lafayette College, Easton, Pennsylvania 18042, USA)

      J. Liquid Chrom. 10, 1277-1290 (1987). TLC on silica, Ag+ impregnated silica with e.g. isopropyl ether - acetic acid 96:4, then with petrol ether - acetic acid 90:10:1. Also chromatography on RP-18 silica. Derivatization with phosphomolybdic acid reagent, for quantification with copper acetate - H3PO4 reagent. Quantification by densitometry.

      Keywords:
      Classification: 13c